Delay‐dependent robust reliable control for uncertain switched neutral systems

This article investigates the robust reliable control problem for a class of uncertain switched neutral systems with mixed interval time‐varying delays. The system under study involves state time‐delay, parameter uncertainties and possible actuator failures. In particular, the parameter uncertainties is assumed to satisfy linear fractional transformation formulation and the involved state delay are assumed to be randomly time varying which is modeled by introducing Bernoulli distributed sequences. The main objective of this article is to obtain robust reliable feedback controller design to achieve the exponential stability of the closed‐loop system in the presence of for all admissible parameter uncertainties. The proposed results not only applicable for the normal operating case of the system, but also in the presence of certain actuator failures. By constructing an appropriate Lyapunov–Krasovskii functional, a new set of criteria is derived for ensuring the robust exponential stability of the closed‐loop switched neutral system. More precisely, zero inequality approach, Wirtinger's based inequality, convex combination technique and average dwell time approach are used to simplify the derivation in the main results. Finally, numerical examples with simulation result are given to illustrate the effectiveness and applicability of the proposed design approach. © 2015 Wiley Periodicals, Inc. Complexity 21: 224–237, 2016     

Tri- and Pentamethine Cyanine Dyes for Fluorescent Detection of α-Synuclein Oligomeric Aggregates

The pathogenesis of Parkinson’s disease that is the second most common neurodegenerative disease is associated with formation of different aggregates of α-synuclein (ASN), namely oligomers and amyloid fibrils. Current research is aimed on the design of fluorescent dyes for the detection of oligomeric aggregates, which are considered to be toxic and morbific spices. Fluorescent properties of series of benzothiazole trimethine and pentamethine cyanines were characterized in free state and in presence of monomeric, oligomeric and fibrilar ASN. The dyes with wide aromatic systems and bulky phenyl and alkyl substituents that are potentially able to interact with hydrophobic regions of oligomeric aggregates were selected for the studies. For majority of studied dyes noticeable changes in fluorescence characteristics were shown in the presence of fibrillar or oligomeric ASN, while the dyes slightly responded on the presence of monomeric protein. For pentamethine cyanine SL-631 and trimethine cyanine SH-299 certain specificity to oligomeric aggregates over fibrils was observed. Using these dyes at 10^?6 M concentration permits the detection of oligomeric ASN in the concentrations range of at least 0.2–2 microM. Pentamethine cyanine SL-631 is proposed as dye for fluorescent detection of oligomeric aggregates of ASN, while trimethine cyanine SH-299 is shown to be a sensitive probe both on oligomeric and fibrillar ASN. It is proposed that wide aromatic system of SL-631 pentamethine dye molecule could better fix on the less dense and structured oligomeric formation, while less bulky and more “crescent-shape” molecule of trimethine dye SH-299 could easier enter into the groove of beta-pleated structure.     

Dependence of silicon position-detector bandwidth on wavelength, power, and bias

We have developed a two-LED wobbler system to generate the spatial displacement of total light intensity on a detector surface, facilitating the acquisition of frequency responses up to 600 kHz with high accuracy. We have used this setup to characterize the low-pass filtering behavior of silicon-based position detectors for wavelengths above 850 nm by acquiring the frequency responses of several quadrant detectors and position-sensitive detectors as functions of wavelength, applied bias voltage, and total light power. We observed an increase in bandwidth for an increase in applied bias voltage and incident-light intensity. The combined effect of these parameters is strongly dependent on the detector used and has significant implications for the use of these detectors in scanning probe and optical tweezers applications.     

Nanomechanical properties of single amyloid fibrils

Amyloid fibrils are traditionally associated with neurodegenerative diseases like Alzheimer's disease, Parkinson's disease or Creutzfeldt-Jakob disease. However, the ability to form amyloid fibrils appears to be a more generic property of proteins. While disease-related, or pathological, amyloid fibrils are relevant for understanding the pathology and course of the disease, functional amyloids are involved, for example, in the exceptionally strong adhesive properties of natural adhesives. Amyloid fibrils are thus becoming increasingly interesting as versatile nanobiomaterials for applications in biotechnology. In the last decade a number of studies have reported on the intriguing mechanical characteristics of amyloid fibrils. In most of these studies atomic force microscopy (AFM) and atomic force spectroscopy play a central role. AFM techniques make it possible to probe, at nanometer length scales, and with exquisite control over the applied forces, biological samples in different environmental conditions. In this review we describe the different AFM techniques used for probing mechanical properties of single amyloid fibrils on the nanoscale. An overview is given of the existing mechanical studies on amyloid. We discuss the difficulties encountered with respect to the small fibril sizes and polymorphic behavior of amyloid fibrils. In particular, the different conformational packing of monomers within the fibrils leads to a heterogeneity in mechanical properties. We conclude with a brief outlook on how our knowledge of these mechanical properties of the amyloid fibrils can be exploited in the construction of nanomaterials from amyloid fibrils.     

100 W Kinetically enhanced copper vapor laser at 10 kHz repetition-rate, high (∼1.5%) efficiency with low (∼1.6 kW/l) specific input power and performance of new resonator configurations

It is established here that kinetically enhanced copper vapor lasers (KE-CVLs) based on large-bore discharge tubes can provide high (>1.4%) efficiency at ∼9-10 kHz rep-rate with very low (<2 kW/l) specific input power. Comparative performance of various large-bore kinetically enhanced copper vapor lasers in the range 45-70 mm is presented for most suitable discharge tube. Maximum output power of ∼100 W was achieved with efficiency of about 1.55% at 10 kHz rep-rate from the 60 mm bore KE-CVL. The pulse to pulse efficiency of the KE-CVL was ∼2%, tube efficiency ∼2.1%, and laser pulse energy was about ∼10 mJ. These results show significant advancement in the laser system as an elemental high temperature CVL due to volumetric scaling and KE-effects combined with very low specific input power of ∼1.65 kW/l as compared to 8-9 kW/l normally required in other kinetically enhanced copper vapor lasers to generate ∼100 W output power. Performance of the KE-CVL with 3 new cavity configurations namely, (1) CAT-EYE resonator (2) hybrid resonator, and (3) modified diffraction coupled resonator with dot mirrors are presented for the first here. CAT-EYE resonator was demonstrated to achieve high misalignment tolerance without significant loss of power. A typical drift in power of ∼5% was observed with misalignment responsible for 40% decline in power in case of standard plane-plane cavity. Effect of resonator misalignment on amplifier output drift was also investigated using CAT-EYE resonator in oscillator-amplifier configuration. In case of using stable-unstable hybrid resonator, high magnification of M ∼ 1500 was realized resulting in extremely low divergence (∼0.08 mrad) beam with modest (∼20%) loss in average power. In case of modified DCR cavity, record power of about 48 W was achieved with beam divergence of about 0.1 mrad on using intra-cavity 2 × 2 array of 4 dot mirrors.     

Growth of gold nanoparticles in human cells

Gold nanoparticles of 20-100 nm diameter were synthesized within HEK-293 (human embryonic kidney), HeLa (human cervical cancer), SiHa (human cervical cancer), and SKNSH (human neuroblastoma) cells. Incubation of 1 mM tetrachloroaurate solution, prepared in phosphate buffered saline (PBS), pH 7.4, with human cells grown to approximately 80% confluency yielded systematic growth of nanoparticles over a period of 96 h. The cells, stained due to nanoparticle growth, were adherent to the bottom of the wells of the tissue culture plates, with their morphology preserved, indicating that the cell membrane was intact. Transmission electron microscopy of ultrathin sections showed the presence of nanoparticles within the cytoplasm and in the nucleus, the latter being much smaller in dimension. Scanning near field microscopic images confirmed the growth of large particles within the cytoplasm. Normal cells gave UV-visible signatures of higher intensity than the cancer cells. Differences in the cellular metabolism of cancer and noncancer cells were manifested, presumably in their ability to carry out the reduction process.     

The use of squaric acid as a scaffold for cofacial phenyl rings

[structure: see text] To examine the possibility of using squaric acid as a scaffold for organizing phenyl rings in a cofacial orientation, we undertook an investigation of the conformational preferences of secondary and tertiary N-phenylsquaramides. In secondary squaramides, the extended ZZ conformation is preferred, while in the N-methyl derivative, the folded EE conformation with cofacial phenyl rings is preferred. This conformational switch is likely driven by a combination of steric and electronic factors.     

Antiparallel arrangement of the helices of vesicle-bound alpha-synuclein

alpha-Synuclein (alphaS) is the main component of Lewy bodies from Parkinson's disease. That alphaS binds to membranes is known, but the conformation it adopts is still unclear. Pulsed EPR on doubly spin-labeled variants of alphaS sheds light on the most likely structure. For alphaS bound to vesicles large enough to accommodate also the extended conformation, an antiparallel helix conformation is found. This suggests that the bent structure shown is the preferred conformation of alphaS on membranes.     

Time, space, and spectrally resolved studies on J-aggregate interactions in zeolite L nanochannels

Temporally and spectrally resolved confocal microscopy has been used to explore the behavior of pyronine intercalated zeolite L crystals at different loadings. The low pyronine loading of 0.6% exhibits photophysical behavior similar to that of the free molecule in solution, indicating molecules are isolated from each other in the crystal channels. The higher loading of 20% results in a dye gradient along the channel axis, and the presence of an additional red-shifted spectroscopic transition, with shorter lifetimes. The new band is assigned to an inline arrangement of the molecules undergoing a J-aggregate-type coupling, a process so far not observed in subnanometer channels.     

Enantioselective synthesis of BMS-911278: a triple reuptake inhibitor

BMS-911278 was identified as a potent triple reuptake inhibitor potentially useful for the treatment of depression. The original racemic synthesis suffered from tedious and low recovery resolution and HPLC separation, as well as low-yielding hazardous N-demethylation at the API step. To support further preclinical studies, a scalable enantioselective synthesis was developed. Herein, we report an efficient asymmetric synthesis of BMS-911278 featuring two key steps: an enantioselective Miyaura reaction and an intramolecular regioselective cyclization.