A spectrophotometric method for quantitative determination of lactulose in pharmaceutical preparations

A simple spectrophotometric assay for the quantification of lactulose in pharmaceutical preparations was developed. The method is based on hydrolysis of lactulose under acidic conditions. The hydrolyzed product reacts with resorcinol, giving absorption peaks at 398 and 480 nm. Both absorption wavelengths can be used for the determination of lactulose. The limit of detection of lactulose at 398 nm and 480 nm was 0.075 μg mL⁻¹ and 0.65 μg mL⁻¹, respectively. The calibration was linear in the range of 5–25 μg mL⁻¹. Analytical conditions were optimized, and the method was validated for analysis of pharmaceutical preparations. The determined amount of lactulose was found to be in good agreement with labeled claims in commercial products. The proposed method is economical, convenient, and suitable for the quantification of lactulose in pharmaceutical preparations. The text was submitted by the authors in English.     

Flow Injection Spectrophotometric Determination of 2,4‐D Herbicide

2,4‐Dichlorophenoxy acetic acid herbicide is spectrophotometrically determined by diazotization method in a flow injection assembly. The method is based on base hydrolysis of herbicides. The hydrolyzed product 2,4‐dichlorophenol is reacted with diazotized sulfanilic acid. The absorbance of the resulting coloured product was measured at 480 nm. The calibration graph is linear over the range of 0.2–20 μgmL^?1, with a relative standard deviation of (RSD) of 7.2% and sample throughput of 90 samples h^?1. The % recovery for determination of 2,4‐dichlorophenoxy acetic acid was found to be 92.0–95.3%. The method is easy, simple and faster than the established chromatographic method. The method was applied for determination of 2,4‐dichlorophenoxy acetic acid herbicide in commercial formulations and for residue determination in fruits and food samples.     

Flow injection spectrophotometric determination of bromoxynil herbicide by diazotization method.

A flow injection spectrophotometric method is proposed for the determination of bromoxynil herbicide. Bromoxynil was hydrolyzed with HCl and the resulting product, 3,5-dibromo-4-hydroxyaniline, was diazotized with nitrite and coupled with aniline. The absorbance of the azo dye was measured at 500 nm. The conditions were optimized for diazotization using FIA. The range of linearity was found to be 0.01 to 5 ppm with a molar absorptivity of 1.27 x 10(5) L mol(-1) cm(-1). The % recovery for the determination of bromoxynil was found to be 91%. The sampling frequency was 80 samples per hour for FIA. The method is simple, fast, and has been successfully applied to the determination of bromoxynil in commercial formulations and food samples.     

The synthesis and spontaneous resolution of 2-(2-thioxothiazolidin-3-yl)thiazole

A single crystalline enantiomer of 2-(2-thioxothiazolidin-3-yl)thiazole 5 has been isolated as the major product of the pyrolysis of 3-(2-thiazolin-2-yl)thiazolidine-2-thione 3 (n = 1); the latter isolated as the sole product of the reaction between thiazolidine-2-thione 2 (n = 1) and diethyl azodicarboxylate (DEAD) in benzene.     

A simple and flexible atom-surface potential with easy-to-handle vibrational spectrum

A highly flexible empirical potential V_p(z) suitable to describe the main features of the laterally averaged atom-surface interaction is presented and an accurate expression for the vibrational spectrum of the atom in V_p(z) is given. This establishes a simple method of inversion of the atom-surface vibrational spectra particularly suitable for levels located in the deepest 9/10 of the potential well. The present method is complementary to the one introduced by Le Roy [5].     

Temperature driven reversible breakdown of pseudomorphism in ultrathin Fe/Cu3Au films

We observe that ultrathin Fe/Cu(3)Au(001) films in the 6-13 A thickness range, beyond the thickness of pseudomorphism breakdown at room temperature, exhibit a temperature dependent structural phase transition in the range T(c) approximately 345-380 K. In the high temperature state the Fe film becomes pseudomorphic, while breakdown of pseudomorphism reversibly occurs as the system is cooled below the transition temperature. The difference between substrate and overlayer thermal expansion coefficient is highlighted as the driving force for the observed transition.     

Viscometric, Acoustical and Spectroscopic Investigation of β-Pinene with Benzene, Toluene, m-Xylene and Mesitylene at 303.15, 308.15 and 313.15 K and Atmospheric Pressure

The densities (ρ), viscosities (η), ultrasonic speeds (u) and spectroscopic data of binary mixtures of benzene, toluene, m-xylene and mesitylene with β-pinene as a common component, over the whole composition range of mole fraction of β-pinene including those of pure components, have been measured at 303.15, 308.15 and 313.15 K, except for the spectroscopic study where the temperature was maintained at 298.15 K. The experimental results deviation in viscosity, deviation in ultrasonic velocity, isentropic compressibility and deviation in isentropic compressibility are discussed in terms of molecular interactions between unlike molecules. The variation of these excess parameters indicates the presence of weak interactions between β-pinene and benzene, toluene, m-xylene and mesitylene molecules. Moreover, the viscosity data are discussed in terms of interaction parameters. The theoretical ultrasonic speed was computed using the Nomoto model, ideal mixing relation, Jacobson’s free length theory and compared with the experimentally measured values. The experimental values are also discussed in terms of FTIR spectroscopy.     

Inelastic effect on the resonance lineshapes in he-graphite scattering

A multiple Debye—Waller effect for bound state resonances in atom scattering was recently proposed to explain the observed width and strength of measured resonances. The model was applied with success to measurement of the specular intensity for He-graphite at low incident energies, and to measurement of the diffracted intensities for D-LiF(001) at grazing incidence. We present new calculations for He-graphite at intermediate incident energy, by which the multiple Debye-Waller attenuation appears completely inadequate to describe experimental the inelastic effect on the resonance lineshapes.     

Metal-mediated self-assembly of protein superstructures: influence of secondary interactions on protein oligomerization and aggregation

We have previously demonstrated that non-self-associating protein building blocks can oligomerize to form discrete supramolecular assemblies under the control of metal coordination. We show here that secondary interactions (salt bridges and hydrogen bonds) can be critical in guiding the metal-induced self-assembly of proteins. Crystallographic and hydrodynamic measurements on appropriately engineered cytochrome cb562 variants pinpoint the importance of a single salt-bridging arginine side chain in determining whether the protein monomers form a discrete Zn-induced tetrameric complex or heterogeneous aggregates. The combined ability to direct PPIs through metal coordination and secondary interactions should provide the specificity required for the construction of complex protein superstructures and the selective control of cellular processes that involve protein-protein association reactions.