A Screening Protocol for Exploring Loop Length Requirements for the Formation of a Three Cytosine-Cytosine+ Base-Paired i-Motif

DNA sequences containing at least four runs of repetitive cytosines can fold into tetra-helical structures called i-Motifs (iMs). The interest in these DNA secondary structures is increasing due to their therapeutical and technological applications. Still, limited knowledge of their folding requirements is currently available. We developed a novel step-by-step pipeline for the systematic screening of putative iM-forming model sequences. Focusing on structures comprising only three cytosine-cytosine⁺ base pairs, we investigated what the minimal lengths of the loops required for formation of an intra-molecular iM are. Our data indicate that two and three nucleotides are required to connect the strands through the minor and majorgrooves of the iM, respectively. Additionally, they highlight an asymmetric behavior according to the distribution of the cytosines. Specifically, no sequence containing a single cytosine in the first and third run was able to fold into intra-molecular iMs with the same stability of those formed when the first and the third run comprise two cytosines. This knowledge represents a step forward toward the development of prediction tools for the proper identification of biologically functional iMs, as well as for the rational design of these secondary structures as technological devices.     

On the 2-rainbow independent domination numbers of some graphs

By suitably adjusting the tropical algebra technique we compute the rainbow independent domination numbers of several infinite families of graphs including Cartesian products \(C_n \Box P_m\) and \(C_n \Box C_m\) for all n and \(m\le 5\), and generalized Petersen graphs P(n, 2) for \(n \ge 3\).

     

60 years of OR in Slovenia: development from a first conference to a vibrant community

The paper provides a short history of the operations research (OR) in Slovenia. Some major events and achievements are mentioned and briefly discussed. The period starts in the year 1964, with the first symposium on OR in Slovenia. In the following decades, there were several important milestones: (1) the start of master’s and Ph.D. studies in OR in 1974, (2) the establishment of SSI-SSOR in 1992 (SSI-SSOR stands for the Slovenian Section for Operational Research within the Slovenian Society of Informatics), and (3) the start of a series of symposia in operations research in 1993. All these activities resulted in an extensive list of publications, projects, and monographs and international connections, proving that OR is still a vibrant field, which facilitates knowledge transfer from pure research to business applications.

     

Electricity consumption prediction using artificial intelligence

Central European Journal of Operations Research - The measurement of electricity consumption at 15-minute granularity, including for households, is increasingly mandated in the EU and this also...