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121.
Abdullah J Ahmad M Heng LY Karuppiah N Sidek H 《Analytical and bioanalytical chemistry》2006,386(5):1285-1292
The stacked-film immobilization of 3-methyl-2-benzothiazolinone hydrazone (MBTH) in hybrid nafion/sol-gel silicate film and
horseradish peroxidase (HRP) in chitosan, performed in order to allow the determination of phenolic compounds, was investigated
via an optical method. The stacked films were deposited onto a microscope glass slide by a spin-coating technique. The quinone
or free radical product formed by the enzymatic reactions of phenolic compounds interacts with MBTH to form azo-dye products,
which can be measured spectrophotometrically at a wavelength of 500 nm. The color intensity of the product was found to increase
in proportion to the phenolic concentration after 5 min of exposure. The response of the biosensor was linear over concentration
ranges of 0.025–0.500, 0.010–0.070 and 0.050–0.300 mM for guaiacol, resorcinol and o-cresol, respectively, and gave detection limits of 0.010, 0.005 and 0.012 mM. The sensor exhibited good sensitivity and stability
for at least two months. 相似文献
122.
A biomineralization process based on the promotion of precipitating agent by the urea-urease enzymatic system is developed to prepare ZnAl layered double hydroxide materials and the effects of the enzymatic reaction parameters on the structural and textural properties of the materials are investigated on the basis of XRD and EM analysis. 相似文献