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41.
Abstract Antibodies were raised in rabbits against purified endonuclease V, the product of the bacteriophage T4 denV gene, which incises DNA at the site of UV-induced pyrimidine dimers. These antibodies cross-reacted with the purified UV-DNA endonuclease from M. luteus in both enzyme-linked immunosorbent assay and Western assays. However, no sequence similarity was detected between the denV gene and M. luteus DNA by hybridization. The two endonucleaes are remarkably similar in enzymatic activity, and their antigenic similarities have been preserved despite differences in their DNA sequences.  相似文献   
42.
Abstract— The pigments in vivo of single cells of Chlorella pyrenoidosa were studied by the microspectrophotometric technique. An accessory recording the first derivative of absorption was used to obtain fine resolution and enhanced accuracy. The results suggest that there are several long-wavelength components of Chl a in vivo. In addition, there seem to be four short-wave forms of Chl a. It is also likely that Chl b exists in vivo in two different forms. The existence of all these forms was demonstrated at room temperature.  相似文献   
43.
带有阈值转换和启动时间的优先权排队   总被引:1,自引:0,他引:1  
在诸如ISDN的通信网络中,多种信息共用一条线路,为了满足不同类型信息的服务质量要求,带有阈值转换的优先权排队系统应是一种合适的模型。本文研究单服务员、两类顾客的带有阈值转换和启动时间的优先权排队系统,首先,分别就抢占和非抢占情形讨论了具有泊松到达、服务时间和启动时间均有指数贩系统,然后就非抢占情况进上步考虑了服务时间和启动时间有一般分布的系统,求出了系统中两类顾客队长的稳态联合概率母函数,藉助这  相似文献   
44.
An accurate, fast, matrix-free implicit method has been developed to solve compressible turbulent How problems using the Spalart and Allmaras one equation turbulence model on unstructured meshes. The mean-flow and turbulence-model equations are decoupled in the time integration in order to facilitate the incorporation of different turbulence models and reduce memory requirements. Both mean flow and turbulent equations are integrated in time using a linearized implicit scheme. A recently developed, fast, matrix-free implicit method, GMRES+LU-SGS, is then applied to solve the resultant system of linear equations. The spatial discretization is carried out using a hybrid finite volume and finite element method, where the finite volume approximation based on a containment dual control volume rather than the more popular median-dual control volume is used to discretize the inviscid fluxes, and the finite element approximation is used to evaluate the viscous flux terms. The developed method is used to compute a variety of turbulent flow problems in both 2D and 3D. The results obtained are in good agreement with theoretical and experimental data and indicate that the present method provides an accurate, fast, and robust algorithm for computing compressible turbulent flows on unstructured meshes.  相似文献   
45.
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