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Abstract Antibodies were raised in rabbits against purified endonuclease V, the product of the bacteriophage T4 denV gene, which incises DNA at the site of UV-induced pyrimidine dimers. These antibodies cross-reacted with the purified UV-DNA endonuclease from M. luteus in both enzyme-linked immunosorbent assay and Western assays. However, no sequence similarity was detected between the denV gene and M. luteus DNA by hybridization. The two endonucleaes are remarkably similar in enzymatic activity, and their antigenic similarities have been preserved despite differences in their DNA sequences. 相似文献
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JOSEPH THOMAS G. P. PHONDKE V. G. TATAKE A. R. GOPAL-AYENGAR 《Photochemistry and photobiology》1970,11(2):85-92
Abstract— The pigments in vivo of single cells of Chlorella pyrenoidosa were studied by the microspectrophotometric technique. An accessory recording the first derivative of absorption was used to obtain fine resolution and enhanced accuracy. The results suggest that there are several long-wavelength components of Chl a in vivo. In addition, there seem to be four short-wave forms of Chl a. It is also likely that Chl b exists in vivo in two different forms. The existence of all these forms was demonstrated at room temperature. 相似文献
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HONG LUO DMITRI SHAROV JOSEPH D. BAUM RAINALD LÖHNER 《International Journal of Computational Fluid Dynamics》2013,27(4):253-270
An accurate, fast, matrix-free implicit method has been developed to solve compressible turbulent How problems using the Spalart and Allmaras one equation turbulence model on unstructured meshes. The mean-flow and turbulence-model equations are decoupled in the time integration in order to facilitate the incorporation of different turbulence models and reduce memory requirements. Both mean flow and turbulent equations are integrated in time using a linearized implicit scheme. A recently developed, fast, matrix-free implicit method, GMRES+LU-SGS, is then applied to solve the resultant system of linear equations. The spatial discretization is carried out using a hybrid finite volume and finite element method, where the finite volume approximation based on a containment dual control volume rather than the more popular median-dual control volume is used to discretize the inviscid fluxes, and the finite element approximation is used to evaluate the viscous flux terms. The developed method is used to compute a variety of turbulent flow problems in both 2D and 3D. The results obtained are in good agreement with theoretical and experimental data and indicate that the present method provides an accurate, fast, and robust algorithm for computing compressible turbulent flows on unstructured meshes. 相似文献
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