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Thomas Georgelin Vincent Maurice Bernard Malezieux Jean-Michel Siaugue Valerie Cabuil 《Journal of nanoparticle research》2010,12(2):675-680
This article deals with the first covalent grafting of an enzyme on twice functionalized γ-Fe2O3@SiO2 core–shell magnetic nanoparticles. First, amino-PEG functionalized nanoparticles were synthesized in order to comply with
non-toxic platforms that would be stable in high concentration and would exhibit chemical groups to allow further coupling
with biomolecules. This approach produces a colloidal suspension of covalently grafted enzymes that remains stable for months
and mimics the enzyme–substrate interactions in solution. Secondly, nanoparticles synthesis and enzyme coupling process were
reported and the catalytic properties of bound enzymes were measured and compared with that of the free one. These new materials
appear to be useful tools for enzymatic catalysis research and may be extended to other biomolecules. Furthermore, magnetic
properties of these materials open the way to separation, purification, and transport under magnetic field. 相似文献
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Sébastien Abramson Liliane Srithammavanh Jean-Michel Siaugue Olivier Horner Xiangzhen Xu Valérie Cabuil 《Journal of nanoparticle research》2009,11(2):459-465
The preparation of core-shell-shell γ-Fe2O3/SiO2/TiO2 nanoparticles of few tens nanometers is performed by successively coating onto magnetic nanoparticles a SiO2 layer and a TiO2 layer, using sol–gel methods. The thickness of the two layers and the aggregation state of the particles can be controlled
by the experimental conditions used for the two coatings. These composite nanoparticles may find application as magnetic photocatalysts,
since they are characterized by their small diameters which allow a good accessibility to the TiO2 shell.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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Aitala EM Amato S Anjos JC Appel JA Ashery D Banerjee S Bediaga I Blaylock G Bracker SB Burchat PR Burnstein RA Carter T Carvalho HS Copty NK Costa I Cremaldi LM Darling C Denisenko K Fernandez A Gagnon P Gerzon S Gobel C Gounder K Halling AM Herrera G Hurvits G James C Kasper PA Kwan S Langs DC Leslie J Lundberg B MayTal-Beck S Meadows B de Mello Neto JR Milburn RH de Miranda JM Napier A Nguyen A d'Oliveira AB O'Shaughnessy K Peng KC Perera LP Purohit MV Quinn B Radeztsky S Rafatian A 《Physical review letters》1996,77(12):2384-2387
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