5-芳胺-4,7-吲唑二酮的合成

以1,4-对醌、芳肼为原料,通过Aza-Nenitzescu反应、氧化银氧化得到吲唑醌,亲核试剂(各种芳香胺)在Ce3 催化下与吲唑醌发生迈克尔加成反应,得到一系列5-芳氨基基取代的吲唑醌.化合物结构经元素分析、IR及1H NMR证实.初步的生理活性研究表明,这些化合物具有良好的抗哮喘活性.     

IMMOBILIZATION OF POTENTIALLY BIOACTIVE MOIETIES ONTO POLYETHER WITH POLY(ETHYLENE GLYCOL)-SULFONATE SPACER

A new reactive graft copolymer, poly(tetramthylene glycol) - graft-w-propyl sodiumsulfonate-poly(ethylene glycol) (PTMG-g-PEG-CH_2CH_2CH_2SO_3~-Na~+), was synthesizedby the cationic polymerization of a-w-bifunctional PEG macromonomer (O?CH_2—PEG——CH_2 CH_2 CH_2 SO_3 Na) and THF. The obtained copolymer exhibits the expected structureas indicated by the result of characterization. Two amino acids (L-arginine, L-tyrosine)were covalently attached to the copolymer after converting the sulfonate group to sulfonylchloride. So the new reactive graft copolymer (PTMG-g-PEG-CH_2CH_2CH_2SO_3~-Na~+) isexpected to be very useful in attachment of potentially bioactive moieties to polymer viaa hydrophilic PEG spacer.     

反应性梳状聚乙二醇改性聚酯薄膜表面及其内皮细胞相容性的研究

以高密度梳状PEG(CPEG)作为表面改性材料, 将PEG末端羟基转化为醛基, 将梳状PEG和线形PEG固定在氨基化的PET膜表面, 并利用表面的反应性醛基进一步固定了氨基酸和整合素配体多肽片段RGD多肽. 红外光谱、 接触角和X射线光电子能谱(XPS)测定结果表明, 该法可有效地固定氨基酸和多肽, 获得模拟细胞膜中多糖-蛋白质复合物结构的特异性功能表面. 对两种不同结构的PEG细胞培养实验结果表明, CPEG比线形PEG(LPEG)具有更好的抗非特异粘附性. 此外, CPEG比LPEG具有更多的活性反应基团, 用PEG末端活性的醛基固定整合素配体多肽片段RGD, 可有效地诱导材料表面的内皮细胞化, 改善材料的细胞相容性.     

A fully automated nanoelectrospray tandem mass spectrometric method for analysis of Caco-2 samples

Caco-2 cells offer a means to rapidly screen permeability of drug candidates, allowing pharmaceutical companies to eliminate candidates unable to cross the intestinal barrier early in the discovery process. This screening process is typically performed by conventional liquid chromatography/tandem mass spectrometry (LC/MS/MS), which can require time-consuming method development. An alternative to LC/MS/MS, automated nanoelectrospray tandem mass spectrometry (nanoESI-MS/MS), is introduced. This novel approach requires an off-line ZipTip desalting step followed by automated nanoESI-MS/MS, using the NanoMate 100 and ESI Chip. In addition to reduced method development time, automated nanoESI-MS/MS also offers no carry-over between samples, low sample consumption, and ease-of-use as compared with conventional pulled-capillary nanoelectrospray. Furthermore, the infusion system described has the potential to be high-throughput. A comparison of Caco-2 samples analyzed both by LC/MS/MS and by automated nanoESI-MS/MS is presented. The permeability and recovery data of the two compounds analyzed in this study obtained from conventional LC/MS/MS and by automated nanoESI-MS/MS were in excellent agreement.     

配体的空间构型及疏水性对钌（Ⅱ）多吡啶配合物与DNA作用的影响

合成了4－氰基苯基咪唑并[5,6-f]邻菲咯啉（CYIP）和2－羧基苯基咪唑并[5, 6-f]邻菲咯啉（COIP）两种新配体及它们的钌混配配合物[Ru(bpy)2CYIP](ClO4)2 ·H2O（Rul）（bpy=2,2′-联吡啶），[Ru(phen)2CYIP](ClO4)2·H2O(Ru2) (phen=1,10-邻菲咯啉），[Ru(bpy)2COIP](ClO4)2·3H2O(Ru3)和[Ru(phen)2COIP] (ClO4)2·H2O(Ru4)，并用红外光谱、紫外光谱、核磁和质谱对它们进行了表征。 通过循环伏安法研究了这些配合物的电化学性质。采用电子吸收光谱、稳态荧光、 圆二色谱和粘度测定研究了配合物与小牛胸腺DNA的相互作用。结果表明配合物 Rul和Ru2通过CYIP配体以插入的方式与DNA结合，而配合物Ru3和Ru4则通过COIP配 体以部分插入的方式与DNA结合。     

Calix [ 4 ] arene Polyaza Derivatives： Novel Effective Neutral Receptors for Cations and α-Amino Acids

IntroductionCalixarenes are one of the most important supra-molecular building blocks, which can be modified byintroducing different functional and/or structural groupsto create a specific interaction between the host and thetarget molecules, such as meta…     

人体血清中痕量铜的PAN分光光度法的测定研究

研究了用Cu2 -1-(2-吡啶偶氮)-2-萘酚(PAN)分光光度法测定人体血清中痕量铜的最佳条件。在HCl-KCl溶液介质中,十二烷基苯磺酸钠存在下,其表观摩尔吸光系数为1.42×104 L.mol-1.cm-1,Cu2 含量在0~2μg/mL范围服从比尔定律,检测限为0.5μg/dm3。操作简便、快速,该法可用于人体血清中痕量铜的测定。     

羧甲基壳聚糖/聚乙烯醇/甘油环氧树脂蛇笼型复合螯合膜的制备及对金属离子的吸附性能

以交联甘油环氧树脂交联的聚乙烯醇(PVA)为笼树脂，羧甲基壳聚糖(CCTS)为蛇树脂制备了具有蛇笼结构的复合螯合膜，研究了其对Cu^2 、Ni^2 、Pb^2 、Fe^3 、Zn^2 ，Hg”^2 、Cd^2 等金属离子的吸附性能，研究表明，该树脂对Cu^2 、Ni^2 、Pb^2 有较好的吸附性能，其中PVA是对Cu^2 的吸附的主要贡献者，而CCTS则是在对Ni^2 的吸附中起主要作用。该树脂可以用于含Cu^2 废水的处理。     

聚酰亚胺LB膜热解制备SiC薄膜的XPS研究

用XPS对沉积在硅基片上的聚酰亚胺LB膜以及由它真空热解制备的SiC薄膜进行了研究 ,并对其形成过程进行了跟踪分析 .XPS结果显示聚酰亚胺LB膜结构均匀 ,质量良好 ;真空热解时 ,约在 6 70℃时LB膜中的C与衬底Si反应形成SiC ;Ar离子溅射深度俄歇谱表明所制备的SiC膜中Si和C浓度成梯度分布 ,说明SiC是由Si和C相互扩散反应形成的     

多吡啶钴(Ⅲ)配合物与Zn2+形成的新型“关-开”荧光探针

近十几年来,对小分子过渡金属配合物与大分子DNA键合与识别机理的研究一直是国际上生物无机化学领域十分活跃的研究课题[1￣3],已发展了一系列具有特定功能的配合物,如DNA结构探针和DNA荧光探针等。与其他类型的金属配合物相比,八面体过渡金属多吡啶配合物具有丰富的光化学和光物理信息,当这些配合物与DNA相互作用时,由于结构匹配或微环境的差异,配合物的光谱特征会出现不同程度的改变,从而达到对DNA的检测。传统的DNA荧光探针有[Ru(bpy)2dppz]2 和[Ru(phen)2dppz]2 (bpy=2,2′-联吡啶,phen=1,10-菲咯啉,dppz=二吡啶[3,2-a∶2′,3′…