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41.
Folding of Synthetic Homogeneous Glycoproteins in the Presence of a Glycoprotein Folding Sensor Enzyme 下载免费PDF全文
Dr. Simone Dedola Dr. Masayuki Izumi Dr. Yutaka Makimura Dr. Akira Seko Dr. Akiko Kanamori Dr. Masafumi Sakono Prof. Dr. Yukishige Ito Prof. Dr. Yasuhiro Kajihara 《Angewandte Chemie (International ed. in English)》2014,53(11):2883-2887
UDP‐glucose:glycoprotein glucosyltransferase (UGGT) plays a key role in recognizing folded and misfolded glycoproteins in the glycoprotein quality control system of the endoplasmic reticulum. UGGT detects misfolded glycoproteins and re‐glucosylates them as a tag for misfolded glycoproteins. A flexible model to reproduce in vitro folding of a glycoprotein in the presence of UGGT in a mixture containing correctly folded, folding intermediates, and misfolded glycoproteins is described. The data demonstrates that UGGT can re‐glucosylate all intermediates in the in vitro folding experiments, thus indicating that UGGT inspects not only final folded products, but also the glycoprotein folding intermediates. 相似文献
42.
The weakly ionic amphiphilic diblock copolymer polystyrene-b-poly(acrylic acid) was synthesized by nitroxy radical-mediated living radical polymerization with precise control of block length, block ratio, and polydispersity. Systematical surface tension experiments and foam formation observations revealed that this polymer was non-surface active under neutral and alkaline (pH 10) conditions, while it was surface active under an acidic condition (pH 3). This result supports our proposed origin of non-surface activity; the image charge repulsion at the air/water interface is essential in addition to very stable micelle formation in the bulk solution. At a higher pH (pH 12), the polymer showed slight surface activity since the added NaOH played a role as an added salt. The critical micelle concentration (cmc) was estimated by static light scattering. Cmc increased with increasing added salt (NaCl) concentration as was observed for other strongly ionic non-surface-active polymers. Hence, this trend is characteristic for non-surface-active polymers. The pH dependence of cmc was minimum at pH 8–10. Since the acrylic acid block is fully ionized under this condition, the strong image charge repulsion at this condition accelerated micelle formation at a low polymer concentration, which consequently decreased cmc. Micelles in bulk solution were confirmed by dynamic light scattering, and the salt concentration and pH dependencies of the hydrodynamic radius of the micelles were also estimated. The pH-responsive non-surface-active/surface-active transition observed in this study strongly supports the fact that the image charge repulsion is an essential factor for non-surface activity in addition to stable micelle formation in solution. 相似文献
43.
Takeshi Kimura Yukiko Izumi Naomichi Furukawa Yoshihiro Minoshima 《Heteroatom Chemistry》1996,7(2):143-147
1,1-Dimethyldibenzo[bc,fg][1,4]silathiapentalene ( 1a ) was prepared by treatment of 1,9-bis(methyl-sulfinyl)dibenzothiophene with EtMgBr or of dibenzothiophene with n-butyllithium, and then with dimethyl dichlorosilane. The structure of 4,4-dimethyl-dibenzo[bc,fg][1,4]silathiapentalene 1-oxide ( 2 ), obtained by oxidation of compound 1a with mCPBA, was determined by X-ray crystallographic analysis. The structure of compound 2 determined experimentally was compared to the structure obtained by semiempirical molecular orbital calculations (AM1). The MO calculations of compound 1a and its phenyl analog 1b were also performed by AM1 to evaluate their structures. © 1996 John Wiley & Sons, Inc. 相似文献
44.
Yukiko Yasuoka Yuichiro Izumi Takashi Fukuyama Haruki Omiya Truyen D. Pham Hideki Inoue Tomomi Oshima Taiga Yamazaki Takayuki Uematsu Noritada Kobayashi Yoshitaka Shimada Yasushi Nagaba Tetsuro Yamashita Masashi Mukoyama Yuichi Sato Susan M. Wall Jeff M. Sands Noriko Takahashi Katsumasa Kawahara Hiroshi Nonoguchi 《Molecules (Basel, Switzerland)》2022,27(3)
Anemia is a major complication of chronic renal failure. To treat this anemia, prolylhydroxylase domain enzyme (PHD) inhibitors as well as erythropoiesis-stimulating agents (ESAs) have been used. Although PHD inhibitors rapidly stimulate erythropoietin (Epo) production, the precise sites of Epo production following the administration of these drugs have not been identified. We developed a novel method for the detection of the Epo protein that employs deglycosylation-coupled Western blotting. With protein deglycosylation, tissue Epo contents can be quantified over an extremely wide range. Using this method, we examined the effects of the PHD inhibitor, Roxadustat (ROX), and severe hypoxia on Epo production in various tissues in rats. We observed that ROX increased Epo mRNA expression in both the kidneys and liver. However, Epo protein was detected in the kidneys but not in the liver. Epo protein was also detected in the salivary glands, spleen, epididymis and ovaries. However, both PHD inhibitors (ROX) and severe hypoxia increased the Epo protein abundance only in the kidneys. These data show that, while Epo is produced in many tissues, PHD inhibitors as well as severe hypoxia regulate Epo production only in the kidneys. 相似文献
45.
Dr. Dinar Abdullin Dr. Hideto Matsuoka Dr. Maxim Yulikov Nico Fleck Dr. Christoph Klein Dr. Sebastian Spicher Dr. Gregor Hagelueken Prof. Dr. Stefan Grimme Prof. Dr. Arne Lützen Prof. Dr. Olav Schiemann 《Chemistry (Weinheim an der Bergstrasse, Germany)》2019,25(37):8820-8828
Pulsed EPR dipolar spectroscopy (PDS) offers several methods for measuring dipolar coupling and thus the distance between electron-spin centers. To date, PDS measurements to metal centers were limited to ions that adhere to the high-field approximation. Here, the PDS methodology is extended to cases where the high-field approximation breaks down on the example of the high-spin Fe3+/nitroxide spin-pair. First, the theory developed by Maryasov et al. (Appl. Magn. Reson. 2006 , 30, 683–702) was adapted to derive equations for the dipolar coupling constant, which revealed that the dipolar spectrum does not only depend on the length and orientation of the interspin distance vector with respect to the applied magnetic field but also on its orientation to the effective g-tensor of the Fe3+ ion. Then, it is shown on a model system and a heme protein that a PDS method called relaxation-induced dipolar modulation enhancement (RIDME) is well-suited to measuring such spectra and that the experimentally obtained dipolar spectra are in full agreement with the derived equations. Finally, a RIDME data analysis procedure was developed, which facilitates the determination of distance and angular distributions from the RIDME data. Thus, this study enables the application of PDS to for example, the highly relevant class of high-spin Fe3+ heme proteins. 相似文献
46.
47.
Atsushi Izumi Masahiro Teraguchi Ryoji Nomura Toshio Masuda 《Journal of polymer science. Part A, Polymer chemistry》2000,38(7):1057-1063
Poly(phenylenevinylene)‐based conjugated polymers with azobenzene groups in the main chains were prepared by the Pd‐catalyzed coupling polymerization of divinylarenes with dihaloarenes. The Pd‐catalyzed coupling polymerization of 4,4′‐divinylazobenzene with dihaloarenes such as 1,3‐dibromobenzene, 1,4‐dibromo‐2,5‐dihexylbenzene, 4,4′‐dibromoazobenzene, and 4,4′‐diiodoazobenzene resulted in polymers with poor solubility. In contrast, soluble polymers containing azobenzene moieties in the main chains were attainable from divinylbenzenes with 4,4′‐dihaloazobenzenes if either or both of the monomers possessed hexyl groups on the aromatic rings. The number‐average molecular weight of the polymer exceeded 10,000 under optimized conditions, and the polymer showed a remarkably redshifted absorption in the visible region (456 nm). 1H NMR and IR spectra supported that the polymers having only trans‐geometry for the double bonds. © 2000 John Wiley & Sons, Inc. J Polym Sci A: Polym Chem 38: 1057–1063, 2000 相似文献
48.
A N(1)-C(5) bridged chiral bicyclic imidazole with a morpholine framework was synthesized from an enantiopure 2-amino alcohol. The resultant imidazole reacted with various electrophiles, including primary and secondary alkyl halides, benzyne, and an electron-deficient aryl halide, to give the corresponding imidazolium salts. Some of the imidazolium salts were found to have potential as the precursor of a chiral N-heterocyclic carbene catalyst; by the direct annulation of an enal and a ketone through the intermediacy of a homoenolate and an activated carboxylate, the target lactone was obtained in an enantiomerically enriched form (up to 66% ee). 相似文献
49.
Izumi T Naitou Y Shimbo Y Takanishi Y Takezoe H Watanabe J 《The journal of physical chemistry. B》2006,110(47):23911-23919
The mesomorphic behavior and phase structure were examined in the mixture of two kinds of dimeric compounds, alpha,omega-bis(4-alkoxyanilinebenzylidene-4'-carbonyloxy)pentane (mOAM5AMOm), by optical microscopy, X-ray diffraction, polarization switching, and second-harmonic generation measurements. One compound is 4OAM5AMO4 with a short terminal alkyl chain that forms a single-layer smectic phase (SmCAs) with a random mixing of spacer and tail groups. Another compound is 16OAM5AMO16 with a long terminal alkyl chain that forms a chiral, anticlinic, and antiferroelectric bilayer phase (SmCAb) with the bent molecules tilted to the bilayer. By mixing these two compounds, the SmCAs phase of 4OAM5AMO4 is easily destabilized, leading to the wide content region of the bilayer phases. In the bilayer regime, three other smectic phases are newly induced. Two of them are antiferroelectric and ferroelectric phases in which the molecules lie perpendicularly with respect to the layer. The other shows no polar response to an external electric field and behaves like a smectic A. The new appearance of these bilayer phases is discussed as a mixing effect of long and short tail groups. 相似文献
50.
Kunishima M Tokaji M Matsuoka K Nishida J Kanamori M Hioki K Tani S 《Journal of the American Chemical Society》2006,128(45):14452-14453
Mere chemical generation of ceramide and related double-chain lipids in the membrane of small unilamellar vesicles (SUVs) induces fusion of the vesicles. The lipids can be successfully prepared by dehydrocondensation between single-chain lipids (fatty acids and sphingosine or its analogues) in a lipid bilayer of the SUV by using a combination of 2-chloro-4,6-dimethoxy-1,3,5-triazine and amphiphilic tertiary amine catalysts, a process that can be compared to a successive enzyme model system for a fatty acyl-CoA synthetase followed by acyltransferase. The SUV spontaneously undergoes membrane fusion upon this internal chemical stimulation by the artificial enzyme system. 相似文献