Evaluation of light scattering properties and chromophore concentrations in skin tissue based on diffuse reflectance signals at isosbestic wavelengths of hemoglobin

Optical Review - We investigate a method to evaluate light-scattering properties and chromophore concentrations in human skin tissue through diffuse reflectance spectroscopy using the reflectance...     

Direct Introduction of a Dimesitylboryl Group Using Base‐Mediated Substitution of Aryl Halides with Silyldimesitylborane

The first dimesitylboryl substitution of aryl halides with a silylborane bearing a dimesitylboryl group in the presence of alkali‐metal alkoxides is described. The reactions of aryl bromides or iodides with Ph₂MeSi?BMes₂ and Na(OtBu) afforded the desired aryl dimesitylboranes in good to high yields and with high borylation/silylation ratios. Selective reaction of the sterically less‐hindered C?Br bond of dibromoarenes provided monoborylated products. This reaction was used to rapidly construct a D‐π‐A aryl dimesityl borane with a non‐symmetrical biphenyl spacer.     

Color adjustment algorithm adapted to the spectral reflectance estimation method

We are developing a daily health monitoring system that uses mobile phones with cameras and analyzes physiological conditions from R, G, and B intensity levels. However, since it is affected by various imaging conditions of the image input, color correction is required for accurate health monitoring. Therefore, we developed and validated a colorcorrection algorithm to derive reliable color information by correcting the spectral reflectance using the Wiener estimation and a color chart.     

Improvement of spatial homogeneity in GdBCO/IBAD-MgO coated conductor

Recent process optimization allows improving homogeneity and a significant increase of J_c in GdBa₂Cu₃O_7-δ deposited on ion-beam assisted deposited MgO template (GdBCO/IBAD-MgO). We applied low-temperature laser scanning microscopy and laser scanning thermo-electric microscopy (LSTEM) to investigate local dissipation and defects simultaneously in recent GdBCO/IBAD-MgO coated conductor. By using high-resolution LSTEM, we could detect current blocking obstacles which are responsible for the large scale local dissipation. Data on the present GdBCO/IBAD-MgO coated conductors point out a significant reduction of current blocking obstacles compared to the previous process. We have shown the improvement of spatial homogeneity in the recent GdBCO/IBAD-MgO coated conductors. Current blocking obstacles are much less densely distributed as compared to previous YBCO/IBAD-GZO coated conductor. Obstacles larger than several micrometer significantly increase local dissipation whereas smaller defects do not influence it noticeably. 2D map of the phase delay component of the thermoelectric voltage lock-in signal is effective to analyze current blocking obstacles having action upon local dissipation.     

α-Trifluoromethyl Chalcones as Potent Anticancer Agents for Androgen Receptor-Independent Prostate Cancer

α-Trifluoromethyl chalcones were prepared and evaluated for their antiproliferative activities against androgen-independent prostate cancer cell lines as well as five additional types of human tumor cell lines. The most potent chalcone 5 showed superior antitumor activity in vivo with both oral and intraperitoneal administration at 3 mg/kg. Cell-based mechanism of action studies demonstrated that 5 induced cell accumulation at sub-G1 and G2/M phases without interfering with microtubule polymerization. Furthermore, several cancer cell growth-related proteins were identified by using chalcone 5 as a bait for the affinity purification of binding proteins.     

In vivo estimation of optical properties of rat liver using single-reflectance fiber probe during ischemia and reperfusion

Optical Review - To quantify the changes in optical properties of in vivo rat liver tissue, we applied diffuse reflectance spectroscopy (DRS) system using single-reflectance fiber probe during...     

In vivo multispectral imaging of the absorption and scattering properties of exposed brain using a digital red–green–blue camera

Optical Review - To evaluate multi-spectral images of the absorption and scattering properties in the cerebral cortex of rat brain, we investigated spectral reflectance images estimated by the...     

Folding of Synthetic Homogeneous Glycoproteins in the Presence of a Glycoprotein Folding Sensor Enzyme

UDP‐glucose:glycoprotein glucosyltransferase (UGGT) plays a key role in recognizing folded and misfolded glycoproteins in the glycoprotein quality control system of the endoplasmic reticulum. UGGT detects misfolded glycoproteins and re‐glucosylates them as a tag for misfolded glycoproteins. A flexible model to reproduce in vitro folding of a glycoprotein in the presence of UGGT in a mixture containing correctly folded, folding intermediates, and misfolded glycoproteins is described. The data demonstrates that UGGT can re‐glucosylate all intermediates in the in vitro folding experiments, thus indicating that UGGT inspects not only final folded products, but also the glycoprotein folding intermediates.