Wiring of redox enzymes on three dimensional self-assembled molecular scaffold

The integration of biological molecules and nanoscale components provides a fertile basis for the construction of hybrid materials of synergic properties and functions. Stable protein 1 (SP1), a highly stable ring shaped protein, was recently used to display different functional domains, to bind nanoparticles (NPs), and to spontaneously form two and three-dimensional structures. Here we show an approach to wire redox enzymes on this self-assembled protein-nanoparticle hybrid. Those hybrids are genetically engineered SP1s, displaying glucose oxidase (GOx) enzymes tethered to the protein inner pore. Moreover, the Au-NP-protein hybrids self-assembled to multiple enzymatic layers on the surface. By wiring the redox enzymes to the electrode, we present an active structure for the bioelectrocatalytic oxidation of glucose. This system demonstrates for the first time a three-dimensional assembly of multiple catalytic modules on a protein scaffold with an efficient electrical wiring of the enzyme units on an electrode surface, thus implementing a hybrid electrically active unit for nanobioelectronic applications.     

Fractionalization noise in edge channels of integer quantum Hall states

A theoretical calculation is presented of current noise which is due charge fractionalization, in two interacting edge channels in the integer quantum Hall state at filling factor ν=2. Because of the capacitive coupling between the channels, a tunneling event, in which an electron is transferred from a biased source lead to one of the two channels, generates propagating plasma mode excitations which carry fractional charges on the other edge channel. When these excitations impinge on a quantum point contact, they induce low-frequency current fluctuations with no net average current. A perturbative treatment in the weak tunneling regime yields analytical integral expressions for the noise as a function of the bias on the source. Asymptotic expressions of the noise in the limits of high and low bias are found.     

Fixed point theorem for reflexive Banach spaces and uniformly convex non positively curved metric spaces

This article generalizes the work of Ballmann and ?wiatkowski to the case of Reflexive Banach spaces and uniformly convex Busemann spaces, thus giving a new fixed point criterion for groups acting on simplicial complexes.     

Second order symmetric duality in multiobjective programming involving cones

An attempt is made to remove certain omissions and inconsistencies in the recent work of Mishra and Lai (European J. Oper. Res., 178:20–26, 2007).     

Vector variational inequalities on Hadamard manifolds involving strongly geodesic convex functions

This paper is intended to study the vector variational inequalities on Hadamard manifolds. Generalized Minty and Stampacchia vector variational inequalities are introduced involving generalized subdifferential. Under strongly geodesic convexity, relations between solutions of these inequalities and a nonsmooth vector optimization problem are established. To illustrate the relationship between a solution of generalized weak Stampacchia vector variational inequality and weak efficiency of a nonsmooth vector optimization problem, a non-trivial example is presented.     

A new iterative method for solving split feasibility problem

In this paper, we construct a new iterative algorithm and show that the newly introduced iterative algorithm converges faster than a number of existing iterative algorithms for contractive-like mappings. We present a numerical example followed by graphs to validate our claim. We prove strong and weak convergence results for approximating fixed points of generalized $\alpha$-nonexpansive mappings. Again we reconfirm our results by an example and table. Further, we utilize our proposed algorithm to solve split feasibility problem.     

Circulating Ligands of the Receptor for Advanced Glycation End Products and the Soluble Form of the Receptor Modulate Cardiovascular Cell Apoptosis in Diabetes

We determined whether plasma concentrations of the receptor for advanced glycation end products (RAGE) and the soluble (s) form of RAGE (sRAGE) in healthy individuals and patients with type 2 diabetes (T2D) modulate vascular remodeling. Healthy individuals and patients with T2D were divided into two age groups: young = <35 years old or middle-aged (36–64 years old) and stratified based on normal glucose tolerance (NGT), impaired (IGT), and T2D. Plasma titers of sRAGE, the RAGE ligands, AGEs, S100B, S100A1, S100A6, and the apoptotic marker Fas ligand Fas(L) were measured by enzyme-linked immunosorbent assay (ELISA). The apoptotic potential of the above RAGE ligands and sRAGE were assessed in cultured adult rat aortic smooth muscle cells (ASMC). In NGT individuals, aging increased the circulating levels of AGEs and S100B and decreased sRAGE, S100A1 and S100A6. Middle-aged patients with T2D presented higher levels of circulating S100B, AGEs and FasL, but lower levels of sRAGE, S100A1 and S100A6 than individuals with NGT or IGT. Treatment of ASMC with either AGEs or S100B at concentrations detected in T2D patients increased markers of inflammation and apoptosis. Responses attenuated by concomitant administration of sRAGE. In middle-aged patients with T2D, lower circulating plasma levels of sRAGE may limit decoy and exogenous trapping of deleterious pro-apoptotic/pro-inflammatory RAGE ligands AGEs and S100B, increasing the risk for diabetic complications.     

Sufficiency in multiobjective subset programming involving generalized type-I functions

In this paper, sufficient optimality conditions for a multiobjective subset programming problem are established under generalized -type-I functions.