Solution of problem of two dissimilar materials bonded at one interface subjected to temperature

A solution of problem of two dissimilar materials bonded at one interface subjected to temperature is derived. To obtain a closed-form solution, a rational mapping function and a complex variable method are used. The coefficients of the homogeneous part of the stress function are expressed by Dunders’ parameters, but loading term of temperature is not expressed by them. As a demonstration, semi-strips bonded at one part at the ends are considered. The each strip is subjected to uniform temperature. Examples of stress distributions are shown. The relations of stress and temperature on the interface are described. Debondings on both sides of the interface are considered. Stress intensity of debonding (SID) is defined, and the values are investigated for various debonding lengths. And the debonding extension or the crack initiation into the material is investigated. The effects of material constants (Dundurs’ parameter) on SID are also investigated. By changing mapping function, other geometries can be analyzed.     

Identification of oligosaccharides from histopathological sections by MALDI imaging mass spectrometry

Direct tissue analysis using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) provides the means for in situ molecular analysis of a wide variety of biomolecules. This technology—known as imaging mass spectrometry (IMS)—allows the measurement of biomolecules in their native biological environments without the need for target-specific reagents such as antibodies. In this study, we applied the IMS technique to formalin-fixed paraffin-embedded samples to identify a substance(s) responsible for the intestinal obstruction caused by an unidentified foreign body. In advance of IMS analysis, some pretreatments were applied. After the deparaffinization of sections, samples were subjected to enzyme digestion. The sections co-crystallized with matrix were desorbed and ionized by a laser pulse with scanning. A combination of α-amylase digestion and the 2,5-dihydroxybenzoic acid matrix gave the best mass spectrum. With the IMS Convolution software which we developed, we could automatically extract meaningful signals from the IMS datasets. The representative peak values were m/z 1,013, 1,175, 1,337, 1,499, 1,661, 1,823, and 1,985. Thus, it was revealed that the material was polymer with a 162-Da unit size, calculated from the even intervals. In comparison with the mass spectra of the histopathological specimen and authentic materials, the main component coincided with amylopectin rather than amylose. Tandem MS analysis proved that the main components were oligosaccharides. Finally, we confirmed the identification of amylopectin by staining with periodic acid-Schiff and iodine. These results for the first time show the advantages of MALDI-IMS in combination with enzyme digestion for the direct analysis of oligosaccharides as a major component of histopathological samples.     

Visualization of acetylcholine distribution in central nervous system tissue sections by tandem imaging mass spectrometry

Metabolite distribution imaging via imaging mass spectrometry (IMS) is an increasingly utilized tool in the field of neurochemistry. As most previous IMS studies analyzed the relative abundances of larger metabolite species, it is important to expand its application to smaller molecules, such as neurotransmitters. This study aimed to develop an IMS application to visualize neurotransmitter distribution in central nervous system tissue sections. Here, we raise two technical problems that must be resolved to achieve neurotransmitter imaging: (1) the lower concentrations of bioactive molecules, compared with those of membrane lipids, require higher sensitivity and/or signal-to-noise (S/N) ratios in signal detection, and (2) the molecular turnover of the neurotransmitters is rapid; thus, tissue preparation procedures should be performed carefully to minimize postmortem changes. We first evaluated intrinsic sensitivity and matrix interference using Matrix Assisted Laser Desorption/Ionization (MALDI) mass spectrometry (MS) to detect six neurotransmitters and chose acetylcholine (ACh) as a model for study. Next, we examined both single MS imaging and MS/MS imaging for ACh and found that via an ion transition from m/z 146 to m/z 87 in MS/MS imaging, ACh could be visualized with a high S/N ratio. Furthermore, we found that in situ freezing method of brain samples improved IMS data quality in terms of the number of effective pixels and the image contrast (i.e., the sensitivity and dynamic range). Therefore, by addressing the aforementioned problems, we demonstrated the tissue distribution of ACh, the most suitable molecular specimen for positive ion detection by IMS, to reveal its localization in central nervous system tissues.     

Effect of Sr addition to La-based perovskite-type oxide as an electrode material for zirconia-based amperometric-type NOx sensor

The sensing characteristics of an amperometric NOx sensor with yttria-stabilized zirconia and a La-based perovskite-type oxide sensing electrode were examined. La_1-xSr_xMO₃ (M = Co, Mn, x = 0, 0.2, 0.4, 0.6) were synthesized using the spray pyrolysis method. The NO₂ response of a sensor fabricated with La_1-xSr_xMnO₃ increased with increasing amount of Sr, and the trend was in contrast to that of the sensors fabricated with La_1-xSr_xCoO₃. LaSr_0.2MnO₃ was determined to be the most appropriate material for the sensor in terms of a high NO₂ response and low O₂ current. In order to discuss the effects of Sr addition to La-based perovskite oxides for the sensor, NO adsorption and desorption properties of oxides and the relationship between the oxidation number of the B-site cation and Sr substitution were examined using temperature-programmed desorption and X-ray photoelectron spectroscopy, respectively.     

Effective and efficient surfactant for CO2 having only short fluorocarbon chains

A previous study (Langmuir2011, 27, 5772) found the fluorinated double-tail sulfogulutarate 8FG(EO)(2) to act as a superefficient solubilizer for water in supercritical CO(2) (W/CO(2)) microemulsions. To explore more economic CO(2)-philic surfactants with high solubilizing power as well as rapid solubilization rates, the effects of fluorocarbon chain length and linking group were examined with sodium 1,5-bis(1H,1H,2H,2H-perfluoroalkyloxy)-1,5-dioxopentane-2-sulfonates (nFG(EO)(2), fluorocarbon chain length n = 4, 6, 8) and sodium 1,4-bis(1H,1H,2H,2H-perfluoroalkyloxy)-1,4-dioxobutane-2-sulfonate (nFS(EO)(2), n = 4, 8). Visual observation and UV-vis spectral measurements with methyl orange as a reporter dye indicated a maximum water-to-surfactant molar ratio (W(0)) in the microemulsions, which was 60-80 for nFG(EO)(2) and 40-50 for nFG(EO)(2). Although it is normally expected that high solubilizing power requires long fluorocarbon surfactant chains, the shortest fluorocarbon 4FG(EO)(2) interestingly achieved the highest W(0) (80) transparent single-phase W/CO(2) microemulsion. In addition, a very rapid solubilization of loaded water into CO(2) was observed for 4FG(EO)(2) even at a high W(0) of ~80.     

盘型激光焊金属蒸汽特征色调-色饱和度-亮度分析法研究

对于大功率盘型激光焊,金属焊件表面在激光束辐射下强烈汽化并形成等离子体状的金属蒸汽羽状物. 该金属蒸汽羽状物可逆向激光束传输,对激光有明显的屏蔽作用, 降低激光辐射至焊件的能量密度,影响焊接效率和质量. 因此研究金属蒸汽特征变化规律及其与焊接质量之间的关联 ,可实现由金属蒸汽特征实时监测激光焊接状态. 以10 kW大功率盘型连续激光焊接304不锈钢钢板为试验对象, 应用高速摄像机摄取金属蒸汽动态图像,将其转换至色调-色饱和度-亮度空间, 提取金属蒸汽面积、激光束受影响路径长度等相关特征量, 以焊缝熔宽的变化作为衡量焊接状态稳定性的参数. 通过金属蒸汽特征值的均值统计和方差分析,试验证明根据金属蒸汽面积和激光束受影响路径长度等金属蒸汽特征可有效地反映熔宽质量, 从而对焊接状况做出动态评估.     

Evolved gas analysis of Ti(C5H5)2Cl2 by means of Li+ ion attachment mass spectrometry

Characterization of the compound Ti(C(5)H(5))(2)Cl(2) was studied using Li(+) ion attachment mass spectrometry (IAMS) as an analytical methodology. Since this target compound is used as an anticancer drug in the treatment of leukemia, accurate and rapid monitoring methods for the determination of titanium drugs in a hospital environment are desirable. A quadrupole mass spectrometry system along with a Li(+) ion attachment technique and a direct inlet probe (DIP) produced the Li(+) adduct of Ti(C(5)H(5))(2)Cl(2), Ti(C(5)H(5))(2)Cl(2)Li(+). The DIP also was used to study the temperature-resolved behavior of this compound. The slope of the plot of signal intensity of Ti(C(5)H(5))(2)Cl(2)Li(+) versus temperature for Ti(C(5)H(5))(2)Cl(2) sublimation from 60 to 100 °C was used to determine an apparent activation energy (E(a)) of 124.43 kJ/mol for the sublimation of Ti(C(5)H(5))(2)Cl(2). This value is comparable to the reported value of 118.8 kJ/mol for molar enthalpy of sublimation of Ti(C(5)H(5))(2)Cl(2). These results demonstrate that the IAMS methodology can be used to study the enthalpy of sublimation for d-metal complex materials.     

Nonlinear fluorescence response driven by ATP-induced self-assembly of guanidinium-tethered tetraphenylethene

Nonlinear fluorescence response, which is particularly important to attain the high signal-to-background ratio, was realized by the aggregation-induced fluorescence increase of guanidinium-tethered tetraphenylethene with ATP.     

Practical synthesis of 4,4,4-trifluorocrotonaldehyde: a versatile precursor for the enantioselective formation of trifluoromethylated stereogenic centers via organocatalytic 1,4-additions

The practical synthesis of 4,4,4-trifluorocrotonaldehyde (1) and its application to enantioselective 1,4-additions are described. The organocatalytic 1,4-addition of 1 with several nucleophiles such as heteroaromatics, alkylthiols and aldoximes afforded the corresponding products, each bearing a trifluoromethylated stereogenic center with high optical purity. A resulting product was converted into an MAO-A inhibitor, befloxatone.