Beta-Carotene Affects the Effects of Heme Oxygenase-1 in Isolated,Ischemic/Reperfused Rat Hearts: Potential Role of the Iron

Beta-carotene (BC) is a well-known antioxidant. However, increasing evidence shows that under severe oxidative conditions, BC can become pro-oxidant, an effect that may be enhanced in the presence of iron (II). In our earlier studies, we observed that despite increasing heme oxygenase-1 (HO-1) levels in the heart, the protective effects of BC have been lost when it was used at a high concentration. Since iron releases from heme as a consequence of HO-1 activity, we hypothesized that the application of an iron-chelator (IC) would reverse the lost cardiac protection associated with an elevated HO-1 level. Thus, in the present study, we investigated the effects of desferrioxiamine (DFO) in isolated, ischemic/reperfused rat hearts after long-term treatment with vehicle or high-dose (HD) BC. Vehicle or 150 mg/bw kg daily doses of BC were administered to the rats for 4 weeks, and then their hearts were removed and subjected to 30 min of global ischemia (ISA) followed by 120 min of reperfusion (REP). During the experiments, cardiac function was registered, and at the end of the REP period, infarct size (IS) and HO-1 expression were measured. The results show that DFO treatment alone during REP significantly ameliorated postischemic cardiac function and decreased IS, although HO-1 expression was not increased significantly. In hearts isolated from BC-treated rats, no cardioprotective effects, despite an elevated HO-1 level, were observed, while DFO administration after ISA resulted in a mild improvement in heart function and IS. Our results suggest that iron could have a role whether BC exerts antioxidant or pro-oxidant effects in ISA/REP-injured hearts.     

Our science and the Covid-19 pandemic—Katalin Karikó’s research idea and her perseverance

Structural Chemistry -     

On the origins of isomorphous replacement in protein crystallography

Structural Chemistry -     

Year of the periodic table: Mendeleev and the others

Structural Chemistry - On the occasion of the Year of the Periodic Table of the Elements, the authors look back at the original discovery, its simultaneity and the difficulties of the discoverers...     

Efficient data assimilation for spatiotemporal chaos: A local ensemble transform Kalman filter

Data assimilation is an iterative approach to the problem of estimating the state of a dynamical system using both current and past observations of the system together with a model for the system’s time evolution. Rather than solving the problem from scratch each time new observations become available, one uses the model to “forecast” the current state, using a prior state estimate (which incorporates information from past data) as the initial condition, then uses current data to correct the prior forecast to a current state estimate. This Bayesian approach is most effective when the uncertainty in both the observations and in the state estimate, as it evolves over time, are accurately quantified. In this article, we describe a practical method for data assimilation in large, spatiotemporally chaotic systems. The method is a type of “ensemble Kalman filter”, in which the state estimate and its approximate uncertainty are represented at any given time by an ensemble of system states. We discuss both the mathematical basis of this approach and its implementation; our primary emphasis is on ease of use and computational speed rather than improving accuracy over previously published approaches to ensemble Kalman filtering. We include some numerical results demonstrating the efficiency and accuracy of our implementation for assimilating real atmospheric data with the global forecast model used by the US National Weather Service.     

Rubbery wound closure adhesives. I. design,synthesis, characterization,and testing of polyisobutylene‐based cyanoacrylate homo‐ and co‐networks

Novel rubbery wound closures containing various proportions and molecular weights of polyisobutylene (PIB) and poly(2‐octyl cyanoacrylate) [P(OctCA)] for potential clinical use were designed, synthesized, characterized, and tested. Homo‐networks were prepared by crosslinking 3‐arm star‐shaped PIBs fitted with terminal cyanoacrylate groups, [Ø(PIB‐CA)₃], and co‐networks by copolymerizing Ø(PIB‐CA)₃ with OctCA using N‐dimethyl‐p‐toluidine (DMT). Neat Ø(PIB‐CA)₃, and Ø(PIB‐CA)₃/OctCA blends, upon contact with initiator, polymerize within seconds to optically transparent strong rubbery co‐networks, Ø(PIB‐CA)₃‐co‐P(OctCA). Homo‐ and co‐network formation was demonstrated by sol/gel studies, and structures and properties were characterized by a battery of techniques. The T_g of P(OctCA) is 58 °C by DSC, and 75 °C by DMTA. Co‐networks comprising 25% Ø(PIB‐CA)₃ (M_n = 2400 g/mol) and 75% P(OctCA) are stronger and more extensible than skin. Short and long term creep studies show co‐networks exhibit high dimensional stability and <6% creep strain at high loading. When deposited on porcine skin co‐networks yield hermetically‐adhering clear rubbery coatings. Strips of porcine skin coated with co‐networks could be stretched and twisted without compromising membrane integrity. The co‐network is nontoxic to L‐929 mouse fibroblasts. © 2015 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2015 , 53, 1640–1651     

Walter B. Gratzer (1932–2021) – molecular scientist and science writer

Structural Chemistry - Walter Gratzer (1932–2021) arrived in England as a child refugee from Nazism, without speaking English, and became one of the most successful and influential science...