Preservation of Immunoreactivity in the Photolabeling of the B72.3 Human Antibody

Abstract— A mild and efficient method of linking a dihydroflavin to the C-17 carboxylic acid side chain of protochlorophyllide, without degradation of the sensitive E ring or loss of magnesium, is described. The appended dihydroflavin was shown to quench the fluorescence of protochlorophyllide. In contrast, a dihydronicotinamide moiety was unable to effect fluorescence quenching. The relevance of these findings to a possible mechanism of action of the enzyme protochlorophyllide reductase is discussed.     

Vibrational corrections to the charge and momentum densities of H2

Vibrational corrections to the charge and momentum densities of H₂ are calculated using the Wang wavefunction and various expressions for the dependence of the effective nuclear charge on the interatomic distance. Both harmonic oscillator and Morse potentials are employed for the vibrational wavefunctions, and excited as well as ground vibrational states are considered. The corrections to the momentum density, though considerably smaller than the charge density corrections, appear to be somewhat more sensitive qualitatively to the choice of both nuclear and electronic wavefunctions.     

Further results on integral generalized inverses of integral matrices

This paper further investigates integral generalized inverses of integral matrices.     

Modelling optical fibres with arbitrary refractive-index profiles

The characteristics of various models of refractive-index profile are examined. Four models are considered: (1) power-law profile, (2) truncated even polynomial profile, (3) truncated all-term polynomial profile, (4) Gauss-Chebychev approximation. The characteristics of the various models are examined with particular reference to the ray-delay times and the shape of the impulse response. The results show that models (1) and (2) are limited in their usefulness in representing practical profiles, while the approximation (4) is easily applied to a variety of practical profiles.     

Semi-empirical corrections to the mixing coefficients for the diatomics-in-molecules method

The diatomics-in-molecules (DIM) method requires information about the wavefunction (i.e. mixing coefficients) and the energy of various states of diatomic fragments. We show here that, if the diatomic energy is obtained from experiment, then it is also important to correct the mixing coefficients by making use of experimental atomic state energies. This point is illustrated by application to the OH⁺(³Σ^?) state manifold.     

Spin-lattice relaxation and a fast T1-map acquisition method in MRI with transient-state magnetization

The magnetization under the spin-lattice relaxation and the nuclear magnetic resonance radiofrequency (RF) pulses is calculated for a signal RF pulse train and for a sequence of multiple RF pulse-trains. It is assumed that the transverse magnetization is zero when each RF pulse is applied. The result expressions can be grouped into two terms: a decay term, which is proportional to the initial magnetization M0, and a recovery term, which has no M0 dependence but strongly depends on the spin-lattice relaxation and the equilibrium magnetization Meq. In magnetic resonance pulse sequences using magnetization in transient state, the recovery term produces artifacts and can seriously degrade the function of the preparation sequence for slice selection, contrast weighting, phase encoding, etc. This work shows that the detrimental effect can be removed by signal averaging in an eliminative fashion. A novel fast data acquisition method for constructing the spin-lattice relaxation (T1) map is introduced. The method has two features: (i) By using eliminative averaging, the curve to fit the T1 value is a decay exponential function rather than a recovery one as in conventional techniques; therefore, the measurement of Meq is not required and the result is less susceptible to the accuracy of the inversion RF pulse. (ii) The decay exponential curve is sampled by using a sequence of multiple pulse-trains. An image is reconstructed from each train and represents a sample point of the curve. Hence a single imaging sequence can yield multiple sample points needed for fitting the T1 value in contrast to conventional techniques that require repeating the imaging sequence for various delay values but obtain only one sample point from each repetition.     

Superlattice Turing structures in a photosensitive reaction-diffusion system

Families of complex superlattice structures, consisting of combinations of basic hexagonal or square patterns, are found in a photosensitive reaction-diffusion system. The structures are induced by simple illumination patterns whose wavelengths are appropriately related to that of the system's intrinsic Turing pattern. Computer simulations agree with the structures and their stability. The technique offers a general approach to generating superlattices for use in information storage and other applications.