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11.
Peixoto L Min B Martins G Brito AG Kroff P Parpot P Angelidaki I Nogueira R 《Bioelectrochemistry (Amsterdam, Netherlands)》2011,81(2):99-103
The biological oxygen demand (BOD) may be the most used test to assess the amount of pollutant organic matter in water; however, it is time and labor consuming, and is done ex-situ. A BOD biosensor based on the microbial fuel cell principle was tested for online and in situ monitoring of biodegradable organic content of domestic wastewater. A stable current density of 282±23mA/m(2) was obtained with domestic wastewater containing a BOD(5) of 317±15mg O(2)/L at 22±2°C, 1.53±0.04mS/cm and pH 6.9±0.1. The current density showed a linear relationship with BOD(5) concentration ranging from 17±0.5mg O(2)/L to 78±7.6mg O(2)/L. The current generation from the BOD biosensor was dependent on the measurement conditions such as temperature, conductivity, and pH. Thus, a correction factor should be applied to measurements done under different environmental conditions from the ones used in the calibration. These results provide useful information for the development of a biosensor for real-time in situ monitoring of wastewater quality. 相似文献
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The accurate in silico identification of T-cell epitopes is a critical step in the development of peptide-based vaccines, reagents, and diagnostics. It has a direct impact on the success of subsequent experimental work. Epitopes arise as a consequence of complex proteolytic processing within the cell. Prior to being recognized by T cells, an epitope is presented on the cell surface as a complex with a major histocompatibility complex (MHC) protein. A prerequisite therefore for T-cell recognition is that an epitope is also a good MHC binder. Thus, T-cell epitope prediction overlaps strongly with the prediction of MHC binding. In the present study, we compare discriminant analysis and multiple linear regression as algorithmic engines for the definition of quantitative matrices for binding affinity prediction. We apply these methods to peptides which bind the well-studied human MHC allele HLA-A*0201. A matrix which results from combining results of the two methods proved powerfully predictive under cross-validation. The new matrix was also tested on an external set of 160 binders to HLA-A*0201; it was able to recognize 135 (84%) of them. 相似文献
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Concise construction of novel bridged bicyclic lactams by sequenced Ugi/RCM/Heck reactions 总被引:1,自引:0,他引:1
Ribelin TP Judd AS Akritopoulou-Zanze I Henry RF Cross JL Whittern DN Djuric SW 《Organic letters》2007,9(24):5119-5122
Herein we report a concise protocol for the diastereoselective synthesis of novel bridged bicyclic lactams from commercially available components by the sequence of Ugi, ring-closing metathesis (RCM), and Heck reactions. X-ray diffraction studies revealed that the bicyclic products contain varying degrees of pyramidalization of the bridgehead nitrogen atom. 相似文献
14.
Irini Michelakaki Stavros Pissadakis 《Applied Physics A: Materials Science & Processing》2009,95(2):453-456
The effect of 193 nm excimer laser radiation on the Knoop hardness and the elastic modulus of an ultra-phosphate glass is
presented here. The experimental results reveal that the glass matrix undergoes a significant softening and volume dilation
process for highly accumulated energy doses, where the Knoop hardness reduces by more than 15%, while following a single photon
absorption rule. Further, during the early stages of the exposure a slight hardening process is observed. Finally, the elastic
modulus is correlated with respect to the measured Knoop hardness. 相似文献
15.
In order to model the experimental compaction of clays as completely as possible, it is necessary to use the hydromechanical coupled three-dimensional theory for saturated porous media. The proposed formulation is based on elastoplasticity, more precisely on modified Cam-clay model. The identification of the parameters of the model is made by special oedometric experiments at steady state. The compaction experiments are simulated with accuracy in transient and steady states and complement those obtained in a recent study based only on steady state. 相似文献
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The 3D-QSAR CoMSIA technique was applied to a set of 458 peptides binding to the five most widespread HLA-A2-like alleles: A*0201, A*0202, A*0203, A*0206 and A*6802. Models comprising the main physicochemical properties (steric bulk, electron density, hydrophobicity and hydrogen-bond formation abilities) were obtained with acceptable predictivity (q
2 ranged from 0.385 to 0.683). The use of coefficient contour maps allowed an A2-supermotif to be identified based on common favoured and disfavoured areas. The CoMSIA definition for the best HLA-A2 binder is as follows: hydrophobic aromatic amino acid at position 1; hydrophobic bulky side chains at positions 2, 6 and 9; non-hydrogen-bond-forming amino acids at position 3; small aliphatic hydrogen-bond donors at position 4; aliphatic amino acids at position 5; small aliphatic side chains at position 7; and small aliphatic hydrophilic and hydrogen-bond forming amino acids at position 8. 相似文献
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Irini Akritopoulou-Zanze Alan Whitehead Jan E. Waters Rodger F. Henry Stevan W. Djuric 《Tetrahedron letters》2007,48(20):3549-3552
We report the synthesis of highly functionalized 3,4-dihydroquinolin-2(1H)-ones via sequential Ugi/acrylanilide photocyclization reactions. The [6π]-photocyclization proceeds in excellent yields and with preferential trans-selectivity for the newly formed ring system. 相似文献
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Expanding the Landscape of Diterpene Structural Diversity through Stereochemically Controlled Combinatorial Biosynthesis
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Dr. Johan Andersen‐Ranberg Dr. Kenneth Thermann Kongstad Dr. Morten Thrane Nielsen Dr. Niels Bjerg Jensen Dr. Irini Pateraki Dr. Søren Spanner Bach Britta Hamberger Prof. Dr. Philipp Zerbe Prof. Dr. Dan Staerk Prof. Dr. Jörg Bohlmann Prof. Dr. Birger Lindberg Møller Prof. Dr. Björn Hamberger 《Angewandte Chemie (International ed. in English)》2016,55(6):2142-2146
Plant‐derived diterpenoids serve as important pharmaceuticals, food additives, and fragrances, yet their low natural abundance and high structural complexity limits their broader industrial utilization. By mimicking the modularity of diterpene biosynthesis in plants, we constructed 51 functional combinations of class I and II diterpene synthases, 41 of which are “new‐to‐nature”. Stereoselective biosynthesis of over 50 diterpene skeletons was demonstrated, including natural variants and novel enantiomeric or diastereomeric counterparts. Scalable biotechnological production for four industrially relevant targets was accomplished in engineered strains of Saccharomyces cerevisiae. 相似文献