Chemical Profiling,Bioactivity Evaluation and the Discovery of a Novel Biopigment Produced by Penicillium purpurogenum CBS 113139

Biobased pigments are environmentally friendly alternatives to synthetic variants with an increased market demand. Production of pigments via fermentation is a promising process, yet optimization of the production yield and rate is crucial. Herein, we evaluated the potential of Penicillium purpurogenum to produce biobased pigments. Optimum sugar concentration was 30 g/L and optimum C:N ratio was 36:1 resulting in the production of 4.1–4.5 AU (namely Pigment Complex A). Supplementation with ammonium nitrate resulted in the production of 4.1–4.9 AU (namely Pigment Complex B). Pigments showed excellent pH stability. The major biopigments in Pigment Complex A were N-threonyl-rubropunctamin or the acid form of PP-R (red pigment), N-GABA-PP-V (violet pigment), PP-O (orange pigment) and monascorubrin. In Pigment Complex B, a novel biopigment annotated as N-GLA-PP-V was identified. Its basic structure contains a polyketide azaphilone with the same carboxyl-monascorubramine base structure as PP-V (violet pigment) and γ-carboxyglutamic acid (GLA). The pigments were not cytotoxic up to 250 μg/mL.     

Controlled free‐radical polymerization of 2‐vinylpyridine in the presence of nitroxides

Bulk free‐radical polymerization of 2‐vinylpyridine (2VP) in the presence of 2,2,6,6‐tetramethylpiperidine‐N‐oxyl (TEMPO) was studied under different conditions (temperature and presence of additives). Linear poly‐(2‐vinylpyridine) with a narrow molecular weight distribution and controllable molecular weight was prepared in the presence of acetic anhydride at 95 °C up to a conversion of 66%. At higher conversions side reactions became very important (pseudoliving polymerization). By applying this procedure, well‐defined random copolymers of 2VP with styrene or tert‐butylmethacrylate as well as block copolymers of 2VP with styrene were synthesized. © 2001 John Wiley & Sons, Inc. J Polym Sci Part A: Polym Chem 39: 2889–2895, 2001     

An intelligent agent negotiation strategy in the electronic marketplace environment

E-commerce will strongly penetrate the market if coupled with appropriate technologies and mechanisms. Mobile agents may enhance the intelligence and improve the efficiency of systems in the e-marketplace. We propose a dynamic multi-lateral negotiation model and construct an efficient negotiation strategy based on a ranking mechanism that does not require a complicated rationale on behalf of the buyer agents. This strategy can be used to extend the functionality of autonomous intelligent agents, so that they quickly reach to an agreement aiming to maximise their owner’s utility. The framework proposed considers both contract and decision issues, is based on real market conditions, and has been empirically evaluated. Moreover, it is shown that in a linear framework like the one we employ, more elaborate ranking mechanisms do not necessarily improve efficiency.     

-bounds for spectral multipliers on graphs

We prove that certain spectral multipliers associated with the discrete Laplacian on graphs satisfying the doubling volume property and the Poincaré inequality are bounded on the Hardy space .

     

Chemical composition and antimicrobial activity of the essential oil of Algerian Phlomis bovei De Noé subsp. bovei

The chemical composition of essential oil obtained by steam distillation of dried aerial parts of Phlomis bovei De Noé subsp. bovei collected from Algeria, was analyzed by GC and GC/MS. Seventy five constituents (corresponding to 86.37% of the total weight) were identified. The main components were: germacrene D, beta-caryophyllene, beta-bournonene, thymol and hexahydrofarnesyl acetone. Furthermore, the antimicrobial activity of the oil was evaluated against six gram (+/-) bacteria and three pathogenic fungi, using the agar dilution technique. It was found that the oil exhibited strong antimicrobial activity against most of the tested microorganisms.     

Proteomics-based discovery of koranimine, a cyclic imine natural product

Nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) are large enzymes responsible for the biosynthesis of medically and ecologically important secondary metabolites. In a previous report, we described a proteomics approach to screen for expressed NRPSs or PKSs from bacteria with or without sequenced genomes. Here we used this proteome mining approach to discover a novel natural product arising from rare adenylation (A) and reductase (Red) domains in its biosynthetic machinery. We also cloned the entire gene cluster and elucidated the biosynthesis of the new compound, which is produced by an unsequenced Bacillus sp. isolated from soil collected in Koran, Louisiana.     

Density functional study of structural, electronic, and optical properties of small bimetallic ruthenium-copper clusters

The structural, electronic, bonding, magnetic, and optical properties of bimetallic [Cu(n)Ru(m)](+/0/-) (n + m ≤ 3; n, m = 0-3) clusters were computed in the framework of the density functional theory (DFT) and time-dependent DFT (TD-DFT) using the full-range PBE0 nonlocal hybrid GGA functional combined with the Def2-QZVPP basis sets. Several low-lying states have been investigated and the stability of the ground state spinomers was estimated with respect to all possible fragmentation schemes. Molecular orbital and population analysis schemes along with computed electronic parameters illustrated the details of the bonding mechanisms in the [Cu(n Ru(m)](+/0/-) clusters. The TD-DFT computed UV-visible absorption spectra of the bimetallic clusters have been fully analyzed and assignments of all principal electronic transitions were made and interpreted in terms of contribution from specific molecular orbital excitations.     

All uncountable cardinals in the Gitik model are almost Ramsey and carry Rowbottom filters

Using the analysis developed in our earlier paper 5 , we show that every uncountable cardinal in Gitik's model of 8 in which all uncountable cardinals are singular is almost Ramsey and is also a Rowbottom cardinal carrying a Rowbottom filter. We assume that the model of 8 is constructed from a proper class of strongly compact cardinals, each of which is a limit of measurable cardinals. Our work consequently reduces the best previously known upper bound in consistency strength for the theory + “All uncountable cardinals are singular” + “Every uncountable cardinal is both almost Ramsey and a Rowbottom cardinal carrying a Rowbottom filter”.     

Unveiling connectivity patterns of categories in complex systems: An application to human needs in urban places

We present a methodology based on weighted networks and dependence coefficients aimed at revealing connectivity patterns between categories. As a case study, it is applied to an urban place and at two spatial levels—neighborhood and square—where categories correspond to human needs. Our results show that diverse spatial levels present different and nontrivial patterns of need emergence. A numerical model indicates that these patterns depend on the probability distribution of weights. We suggest that this way of analyzing the connectivity of categories (human needs in our case study) in social and ecological systems can be used to define new strategies to cope with complex processes, such as those related to transition management and governance, urban-making, and integrated planning.