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21.
Copolymer composition, distribution and molecular size of the comonomer influence the final properties of polymer materials. Such influence can be followed from the effect on the chain conformation in solution determined from the scaling law between radius of gyration and molecular weight. 相似文献
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Lucía Tapia Yolanda Prez Michael Bolte Josefina Casas Jordi Sol Roberto Quesada Ignacio Alfonso 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(36):12595-12598
Acidic microenvironments in solid tumors are a hallmark of cancer. Inspired by that, we designed a family of pseudopeptidic cage‐like anionophores displaying pH‐dependent activity. When protonated, they efficiently bind chloride anions. They also transport chloride through lipid bilayers, with their anionophoric properties improving at acidic pH, suggesting an H+/Cl? symport mechanism. NMR studies in DPC micelles demonstrate that the cages bind chloride within the lipid phase. The chloride affinity and the chloride‐exchange rate with the aqueous bulk solution are improved when the pH is lowered. This increases cytotoxicity towards lung adenocarcinoma cells at the pH of the microenvironment of a solid tumor. These properties depend on the nature of the amino‐acid side chains of the cages, which modulate their lipophilicity and interactions with the cell membrane. This paves the way towards using pH as a parameter to control the selectivity of cytotoxic ionophores as anticancer drugs. 相似文献
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Javier Alcaraz Nuria RamónJosé L. Ruiz Inmaculada Sirvent 《European Journal of Operational Research》2013
The existence of alternate optima for the DEA weights may reduce the usefulness of the cross-efficiency evaluation, since the ranking provided depends on the choice of weights that the different DMUs make. In this paper, we develop a procedure to carry out the cross-efficiency evaluation without the need to make any specific choice of DEA weights. The proposed procedure takes into consideration all the possible choices of weights that all the DMUs can make, and yields for each unit a range for its possible rankings instead of a single ranking. This range is determined by the best and the worst rankings that would result in the best and the worst scenarios of each unit across all the DEA weights of all the DMUs. This approach might identify good/bad performers, as those that rank at the top/bottom irrespective of the weights that are chosen, or units that outperform others in all the scenarios. In addition, it may be used to analyze the stability of the ranking provided by the standard cross-efficiency evaluation. 相似文献
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Alberto Blzquez-Moraleja Ines Senz-de-Santa María María D. Chiara Delia lvarez-Fernndez Inmaculada García-Moreno Ruth Prieto-Montero Virginia Martínez-Martínez Iigo Lpez Arbeloa Jose Luis Chiara 《Chemical science》2020,11(4):1052
The first fluorescent probes that are actively channeled into the mitochondrial matrix by a specific mitochondrial membrane transporter in living cells have been developed. The new functional probes (BCT) have a minimalist structural design based on the highly efficient and photostable BODIPY chromophore and carnitine as a biotargeting element. Both units are orthogonally bonded through the common boron atom, thus avoiding the use of complex polyatomic connectors. In contrast to known mitochondria-specific dyes, BCTs selectively label these organelles regardless of their transmembrane potential and in an enantioselective way. The obtained experimental evidence supports carnitine–acylcarnitine translocase (CACT) as the key transporter protein for BCTs, which behave therefore as acylcarnitine biomimetics. This simple structural design can be readily extended to other structurally diverse starting F-BODIPYs to obtain BCTs with varied emission wavelengths along the visible and NIR spectral regions and with multifunctional capabilities. BCTs are the first fluorescent derivatives of carnitine to be used in cell microscopy and stand as promising research tools to explore the role of the carnitine shuttle system in cancer and metabolic diseases. Extension of this approach to other small-molecule mitochondrial transporters is envisaged.A BODIPY derivative of carnitine enters mitochondria regardless of their membrane potential and in an enantioselective way through a specific mitochondrial membrane transporter in living cells. 相似文献
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Mireia Casasampere Eduardo Izquierdo Josefina Casas Jos Luís Abad Xiao Liu Ruijuan Xu Cungui Mao Young-Tae Chang Antonio Delgado Gemma Fabrias 《Chemical science》2020,11(48):13044
The use of intact cells in medical research offers a number of advantages over employing cell-free systems. In diagnostics, cells isolated from liquid biopsies can be directly used, speeding up the time of analysis and diminishing the risk of protein degradation by sample manipulation. In drug discovery, studies in live cells take into account aspects neglected in cell-free systems, such as uptake, metabolization, and subcellular concentration by compartmentalization of potential drug candidates. Therefore, probes for studies in cellulo are of paramount importance. Acid ceramidase (AC) is a lysosomal enzyme that hydrolyses ceramides into sphingoid bases and fatty acids. The essential role of this enzyme in the outburst and progress of several diseases, some of them still incurable, is well sustained. Despite the great clinical relevance of AC as a biomarker and therapeutic target, the specific monitoring of AC activity in live cells has remained elusive due to the concomitant existence of neutral and alkaline ceramidases. In this work, we report that 1-deoxydihydroceramides are exclusively hydrolysed by AC. Using N-octanoyl-18-azidodeoxysphinganine as a probe and a BODIPY-substituted bicyclononyne, we show the click-reliant predominant staining of lysosomes, with extra-lysosomal labeling also occurring in some cells. Importantly, using pharmacological and genetic tools together with high resolution mass spectrometry, we demonstrate that both lysosomal and extra-lysosomal staining are AC-dependent. These findings are translated into the specific flow cytometry monitoring of AC activity in intact cells, which fills an important gap in the field of diseases linked to altered AC activity.The use of intact cells in medical research offers a number of advantages over employing cell-free systems. 相似文献
27.
ABSTRACTA class of semilinear parabolic reaction diffusion equations with multiple time delays is considered. These time delays and corresponding weights are to be optimized such that the associated solution of the delay equation is the best approximation of a desired state function. The differentiability of the mapping is proved that associates the solution of the delay equation to the vector of weights and delays. Based on an adjoint calculus, first-order necessary optimality conditions are derived. Numerical test examples show the applicability of the concept of optimizing time delays. 相似文献
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Inmaculada De La Calle Noelia Cabaleiro Isela Lavilla 《International journal of environmental analytical chemistry》2013,93(14):1401-1411
A simple, fast and reliable method is described for the quantitative extraction of Sb and Co from inorganic environmental matrices containing variable amounts of silicates prior to their determination by electrothermal-atomic absorption spectrometry. The method is based on the ultrasound-assisted extraction of both elements using the mixture 20% v/v HF + 20% v/v HNO3 as extractant. The extraction procedure was carried out in closed Eppendorf vials immersed in a cup-horn sonoreactor for 20?min. Once extraction has been accomplished, the supernatant liquid is separated from the solid phase and subsequently transferred into the autosampler of the instrument. A two-level full factorial design (24) was applied for screening optimisation of the variables influencing the ultrasonic extraction. These variables were: sonication time; amplitude of the ultrasound energy; nitric and hydrofluoric acid concentrations. MLDs were 0.20 and 0.06?µg?·?g?1 for Sb and Co, respectively. Between-batch precision values, expressed as relative standard deviations (n?=?3), were less than 5.5 and 9.6% for Sb and Co, respectively. The method was evaluated using a wide variety of inorganic certified reference materials, such as SRM 2702 (Marine Sediment), SRM 2782 (Industrial Sludge), BCR 176R (Fly Ash), SRM 1633b (Coal Fly Ash) SRM 2710 (Montana Soil) and SRM 2711 (Montana Soil). 相似文献