The role of the PsbU subunit in the light sensitivity of PSII in the cyanobacterium Synechococcus 7942

In the present study we investigated the role of the PsbU subunit in the electron transport characteristics and light sensitivity of the Photosystem II complex. The experiments were performed by using an earlier characterized PsbU-less mutant of the cyanobacterium Synechococcus PCC 7942, which has enhanced antioxidant capacity (Balint et al. FEBS Lett. 580 (2006) 2117-2122). Flash induced Chl fluorescence measurements in the presence and absence of the electron transport inhibitor DCMU showed that both the S(2)Q(A)(-) and the S(2)Q(B)(-) recombination is slowed down in the PsbU mutant relative to the WT strain. Thermoluminescence measurements confirmed the increased stability of the S(2)Q(A)(-) and S(2)Q(B)(-) charge pairs by showing an increased peak temperature of Q and B bands, which were measured in the presence and absence of DCMU, respectively. In addition, the intensity of the TL bands is also increased in the PsbU mutant (≈1.7 times for the B band), as compared to the WT. The PsbU mutant shows enhanced loss of Photosystem II activity under exposure to high light intensity both in the absence and presence of the protein synthesis inhibitor lincomycin. It is concluded from the data that the lack of the PsbU subunit in Synechococcus PCC 7942 affects the energetic stability of the S(2)Q(A)(-) and S(2)Q(B)(-) charge pairs by modifying both the PSII donor and acceptor side components. This effect is most likely caused by structural changes in the vicinity of the Mn cluster and in the inner part of the PSII complex, which are induced by the lack of the PsbU subunit from the lumenal part of the complex. The light sensitivity of Photosystem II in Synechococcus 7942 in the absence of the PsbU subunit is likely due to reactive oxygen species, which are produced as a consequence of disturbed donor side structure and/or due to the modified energetic properties of the primary radical pair.     

Generation of a stable low-frequency squeezed vacuum field with periodically poled KTiOPO4 at 1064 nm

We report the generation of a stable continuous-wave low-frequency squeezed vacuum field with a squeezing level of 7.4+/-0.1 dB at 1064 nm, the wavelength at which laser-interferometric gravitational wave (GW) detectors operate, using periodically poled KTiOPO4 (PPKTP) in a subthreshold optical parametric oscillator. The squeezing was observed in a broad band of frequencies above 700 Hz where the sensitivity of the currently operational GW detectors is limited by shot noise. PPKTP has the advantages of higher nonlinearity, smaller pump-induced seed absorption, and wider temperature tuning range than alternative nonlinear materials such as MgO-doped or periodically poled LiNbO3, and is, therefore, an excellent material for generation of squeezed vacuum fields for application to laser interferometers for GW detection.     

Synthesis of Silolide Dianions via Reduction of Dichlorosiloles: Important Role of the Solvent

The reduction of 1,1-dichloro-2,5-bistrimethylsilyl-3,4-diphenylsilole to silolide dianion by alkali metals was investigated. As previously demonstrated, the outcome of the reaction depends strongly on the applied alkali metal, solvent, reaction conditions, and substituent pattern. We showed that lithium is a powerful reducing agent in THF or DME solvents, the reaction is even faster than the same reaction with sodium. The X-ray structures of the corresponding dilithio and disodium silolide dianion were investigated, interestingly recrystallization of the dilithio salt results in a coordination polymer. In order to support the synthetic work DFT calculations were performed.     

Veranstaltung des AKCC in der GDCh: Frauenkarrieren ‐ gestern,heute, morgen

Das Potenzial von Frauen in wissenschaftlichen und technischen Berufen ausschöpfen ist ein zentrales Thema der Veranstaltung „Women in Science — Careers in History, Presence and Future”︁, die am 1. November in Köln stattfindet.     

ChemInform Abstract: Hydrodesulfurization of FeS+: Predominance of Kinetic over Thermodynamic Control.

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