Raman spectroscopy of topotecan, an inhibitor of DNA topoisomerase I

Topotecan (TPT), a water-soluble derivative of camptothecin (inhibitor of human DNA topoiomerase I), has found wide application in cancer chemotherapy. The central problem in using topotecan is the presence of lactone rings in its molecules, which undergo hydrolysis at a physiological pH yielding an inactive and even toxic form of the drug. The analysis of Raman spectra of TPT in H₂O and D₂O solutions made it possible to assign the spectral bands to the vibrations of particular molecular groups. Spectral features indicative of the opening of the lactone rings of the TPT molecules, deprotonation of the hydroxyl groups in their quinoline fragments, and of possible participation of the hydroxyl and carbonyl groups in H bonding are found. The data obtained are necessary to study the molecular mechanisms of TPT-DNA interaction and the formation of ternary complexes between TPT, DNA, and DNA topoisomerase I.     

VIBRATION AND STABILITY OF ANNULAR PLATES IN NON-LINEAR CREEP CONDITIONS

This paper presents a study on the influence of the physical non-linearity of material in creep conditions on vibration and stability of non-uniform annular plates subjected to a follower force radially distributed at the outer edge. The stability analysis requires first the calculation of a membrane stress distribution and then the application of the kinetic stability criterion, making use of small superposed vibrations. The differential equations of the membrane and vibration states have been integrated by means of the transfer matrix method which allowed the determination of the relationships between the real and imaginary parts of the complex frequency and compressive force (characteristic curves). The results have been presented in numerous figures.     

Dynamical Characteristics of Traveling Wave Packets of Total Electron Content Disturbances

Using the COPHASE method and the GPS interferometry method for travelling ionospheric disturbances, we analyze in detail the spatio-temporal properties of travelling wave packets (TWP) of total electron content (TEC) disturbances. The analysis is performed on the example of a clearest TWP manifestation observed in California, USA, in October 18, 2001, using the GLOBDET technique, developed at the Institute of Solar-Terrestrial Physics of the Siberian Branch of RAS for global detection and monitoring of natural and technogenic ionospheric disturbances on the basis of TEC variations retrieved from the global network of GPS receivers. In the time domain, TWPs are quasi-periodic TEC oscillations of duration about 1 h, period of 10–20 min, and amplitude exceeding that of the background TEC fluctuations by at least one order of magnitude. The velocity and direction of TWP motion are similar to those of mid-latitude mesoscale travelling ionospheric disturbances, as obtained earlier from the analysis of phase parameters of HF radio signals and the signals of geostationary satellites and discrete space radio sources.     

Temperature and junction-type dependency of Andreev reflection in MgB2

We studied the voltage and temperature dependency of the dynamic conductance of normal metal-MgB₂ junctions obtained either with the point-contact technique (with Au and Pt tips) or by making Ag-paint spots on the surface of MgB₂ samples. The fit of the conductance curves with the generalized BTK model gives evidence of pure s-wave gap symmetry. The temperature dependency of the gap, measured in Ag-paint junctions (dirty limit), follows the standard BCS curve with 2Δ/k_BT_c=3.3. In out-of-plane, high-pressure point-contacts we obtained almost ideal Andreev reflection characteristics showing a single small s-wave gap Δ=2.6±0.2 meV (clean limit).     

Towards Treating Correlations in Bose Condensates

 We use the adiabatic hyperspheric expansion and the Faddeev decomposition of the wave function with only s-waves. We derive for a fixed hyperradius an integro-differential equation for the angular eigenvalue and wave function. The correlations lower the interaction energy for N = 20 by about a factor of 5. Received October 22, 2001; accepted for publication November 5, 2001     

Coherent control of acoustic vibrations in metal nanoparticles and thin films with sequences of femtosecond pulses: Harmonic-oscillator model

A harmonic oscillator model is used to demonstrate the possibility of coherent control of acoustic vibrations of metal nanoparticles and thin films with sequences of femtosecond laser pulses. When the interval between the pulses in such a sequence is chosen equal to the oscillation period of the expansion mode of a nanoscale system, the relevant acoustic vibrations can be excited in a resonant and selective way. Sequences of femtosecond pulses with picosecond time intervals between the pulses are shown to be ideally suited for a resonant excitation and coherent control of acoustic modes of silver nanoparticles.     

Three-dimensional mapping of cholinergic molecules by confocal laser scanning microscopy in sea urchin larvae.

Confocal laser scanning microscopy (CLSM) was used to examine molecules related to the cholinergic neurotransmission system and detected at all the larval stages of Paracentrotus lividus, by histochemical and immunohistochemical methods. CLSM, providing spatial resolution of the cells located both at the larval surface and at depth, allows a complete mapping in a three-dimensional volumetric frame. At early larval stages acetylcholinesterase- as well as choline acetyltransferase-like molecules were found mainly in the gut wall cells, and along the ciliary bands of the arms, together with muscarinic acetylcholine receptors. At perimetamorphic stages, cholinergic molecules were present in the ciliate strands along the arms, in the larval body and in the rudiment. At metamorphosis, positivity to cholinergic molecules translocated to the juvenile, where a high frequency of mAChR- and ChAT-like positive cells was found.     

On mechanical characteristics of nanocrystals

The dependence of the elastic moduli of a nanocrystal on its size is investigated theoretically with reference to a two-dimensional single-crystal strip. It is shown that the uncertainty (of a fundamental nature) in the size of a nanocrystal causes the determination of many of its mechanical characteristics to be ambiguous. It is found that the Cauchy-Green relations are modified and the elastic-constant tensor ceases to be symmetric; the size and shape of a nanocrystal render its mechanical properties more anisotropic. For a single-crystal strip, the Poisson ratio decreases and the Young modulus increases with decreasing thickness of the strip; in the case of a very thin crystal film (two atomic layers thick), these elastic moduli can differ from their macroscopic values by a factor of two. The size effects which make the continuum elasticity theory inapplicable to nanocrystals are estimated. The size effects that occur when the molecular dynamics method is applied for modeling macroscopic objects are also discussed.     

Ground-state energy of pionic hydrogen to one loop

We investigate the ground-state energy of the atom (pionic hydrogen) in the framework of QCD + QED. In particular, we evaluate the strong energy-level shift. We perform the calculation at next-to-leading order in the low-energy expansion in the framework of the relevant effective field theory. The result provides a relation between the strong energy shift and the pion-nucleon S-wave scattering lengths - evaluated in pure QCD - at next-to-leading order in isospin-breaking and in the low-energy expansion. We compare our result with available model calculations. Received: 11 June 2002 / Published online: 9 October 2002