全文获取类型
收费全文 | 127篇 |
免费 | 4篇 |
专业分类
化学 | 78篇 |
数学 | 8篇 |
物理学 | 45篇 |
出版年
2022年 | 1篇 |
2021年 | 1篇 |
2020年 | 2篇 |
2017年 | 3篇 |
2016年 | 5篇 |
2015年 | 1篇 |
2013年 | 5篇 |
2012年 | 4篇 |
2011年 | 7篇 |
2010年 | 5篇 |
2009年 | 3篇 |
2008年 | 4篇 |
2007年 | 6篇 |
2006年 | 4篇 |
2005年 | 5篇 |
2004年 | 8篇 |
2003年 | 7篇 |
2002年 | 7篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1996年 | 6篇 |
1995年 | 4篇 |
1994年 | 7篇 |
1993年 | 5篇 |
1992年 | 1篇 |
1991年 | 3篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 2篇 |
1981年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1969年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有131条查询结果,搜索用时 46 毫秒
71.
Joseph R. Lakowicz Peter A. Koen Henryk Szmacinski Ignacy Gryczynski Józef Kuśba 《Journal of fluorescence》1994,4(1):117-136
Time-resolved fluorescence spectroscopy is presently regarded as a research tool in biochemistry, biophysics, and chemical physics. Advances in laser technology, the development of long-wavelength probes, and the use of lifetime-based methods, are resulting in the rapid migration of timeresolved fluorescence to the clinical chemistry lab, the patient's bedside, and even to the doctor's office and home health care. Additionally, time-resolved imaging is now a reality in fluorescence microscopy and will provide chemical imaging of a variety of intracellular analytes and/or cellular phenomena. Future horizons of state-of-the-art spectroscopy are also described. Two photon-induced fluorescence provides an increased information content to time-resolved data. Two photoninduced fluorescence, combined with fluorescence microscopy and time-resolved imaging, promises to provide detailed three-dimensional chemical imaging of cells. Additionally, it has recently been demonstrated that the pulses from modern picosecond lasers can be used to quench and/or modify the excited-state population by stimulated emission since the stimulated photons are directed along the quenching beam and are not observed. The phenomenon of light quenching should allow a new class of multipulse time-resolved fluorescence experiments, in which the excited-state population is modified by additional pulses to provide highly oriented systems. 相似文献
72.
Joseph R. Lakowicz Józef Kuśba Yibing Shen Joanna Malicka Sabato D'Auria Zygmunt Gryczynski Ignacy Gryczynski 《Journal of fluorescence》2003,13(1):69-77
We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4',6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Förster distance from 35 Å an to an apparent value of 166 Å. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance. 相似文献
73.
Ignacy Z. Siemion 《Magnetic resonance in chemistry : MRC》1976,8(8):432-435
The amounts of boat (A) and planar (B) forms for the equilibrium state in D2O and CD3OD/d6-DMSO (1:1) were calculated for seven proline-containing cyclic dipeptides from their 13C n.m.r. spectra. For c-Pro-Val the thermodynamic functions for the conversion between the boat and planar forms have been obtained from measurements of equilibrium constants at different temperatures. 相似文献
74.
75.
Advances in Surface-Enhanced Fluorescence 总被引:4,自引:0,他引:4
Lakowicz JR Geddes CD Gryczynski I Malicka J Gryczynski Z Aslan K Lukomska J Matveeva E Zhang J Badugu R Huang J 《Journal of fluorescence》2004,14(4):425-441
We report recent achievements in metal-enhanced fluorescence from our laboratory. Several fluorophore systems have been studied on metal particle-coated surfaces and in colloid suspensions. In particular, we describe a distance dependent enhancement on silver island films (SIFs), release of self-quenching of fluorescence near silver particles, and the applications of fluorescence enhancement near metalized surfaces to bioassays. We discuss a number of methods for various shaped silver particle deposition on surfaces. 相似文献
76.
Geddes CD Gryczynski I Malicka J Gryczynski Z Lakowicz JR 《Combinatorial chemistry & high throughput screening》2003,6(2):109-117
Metallic surfaces and particles can have dramatic effects on fluorescence, including localized excitation, increased quantum yields, increased photostability and increased distances for resonance energy transfer (RET), and directional emission. While all these effects have not yet been realized in a single system, metal-enhanced fluorescence promises to provide the next generation of high sensitivity fluorescence assays for low copy number detection of biochemical species. 相似文献
77.
Smith DS Kostov Y Rao G Gryczynski I Malicka J Gryczynski Z Lakowicz JR 《Journal of fluorescence》2005,15(6):895-900
Detection limitations for fluorescence methods are normally determined by the phenomenon itself rather than the sensitivity
of the instrumentation. These limitations make it necessary to have high sensitivity, high cost equipment causing fluorescence
methods to remain lab-oriented. Alleviation of the limitations can be achieved through the phenomenon of surface plasmon-coupled
emission (SPCE), which displays enhanced, directional, polarized fluorescence. Here we present the excitation of SPCE from
Rhodamine B with a light-emitting diode (LED). Incorporating the gains in sensitivity due to SPCE with LED excitation, it
could be possible to design low-cost, high-sensitivity sensors that would allow measurements to be performed in the field. 相似文献
78.
Ignacy Kotlarski 《Annali di Matematica Pura ed Applicata》1966,74(1):129-134
Summary The aim of this paper is to prove the following theorem about characterization of probability distributions in Hilbert spaces:Theorem. — Let x1, x2, …, xn be n (n≥3) independent random variables in the Hilbert spaceH, having their characteristic functionals fk(t) = E[ei(t,x
k)], (k=1, 2, …, n): let y1=x1 + xn, y2=x2 + xn, …, yn−1=xn−1 + xn.
If the characteristic functional f(t1, t2, …, tn−1) of the random variables (y1, y2, …, yn−1) does not vanish, then the joint distribution of (y1, y2, …, yn−1) determines all the distributions of x1, x2, …, xn up to change of location. 相似文献
79.
80.
Ignacy Gryczynski Józef Kuśba Zygmunt Gryczynski Henryk Malak Joseph R. Lakowicz 《Journal of fluorescence》1998,8(3):253-261
We examined the emission wavelength-dependent anisotropies of the solvent-sensitive fluorophore 4-dimethylamino-4-cyanostilbene (DCS) under conditions of light quenching by polarized time-delayed quenching pulses. Illumination on the long-wavelength side of the emission spectrum with time-delayed light pulses resulted in a progressive decrease in the emission anisotropy as the observation wavelength increased toward the stimulating wavelength. The anisotropy changes of DCS were most wavelength dependent when spectral relaxation occurred during the excited-state lifetime. Light quenching of DCS in a low-viscosity solvent revealed no wavelength-dependent anisotropies. Control measurements using a solvent-insensitive fluorophore did not show any wavelength-dependent anisotropy with light quenching. The data for DCS can be explained by a model which allows wavelength-selective quenching of the long-wavelength emission formed by time-dependent spectral relaxation. These results indicate that polarized light quenching can be used to study systems which display multiple emissions and/or time-dependent spectral shifts. 相似文献