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The separation of wide molecular mass (Mr) ranges of macromolecules using frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) has been improved by implementing a combination of field and flow programming. In this first implementation, field strength (governed by the cross flow-rate through the membrane-covered accumulation wall) is decreased with time to obtain faster elution and improved detection of the more strongly retained (high Mr) materials. The channel outlet flow-rate is optionally held constant, increased, or decreased with time. With circulation of the flow exiting the accumulation wall to the inlet frit, the dual programming of cross flow and channel outlet flow could be implemented using just two pumps. With this flow configuration, the channel outlet flow-rate is always equal to the channel inlet flow-rate, and these may be programmed independently of the cross flow-rate through the membrane. FI-AFlFFF retains its operational advantage over conventional asymmetrical flow FFF (AFlFFF). Unlike conventional AFlFFF, FI-AFlFFF does not require time consuming, and experimentally inconvenient, sample focusing and relaxation steps involving valve switching and interruption of sample migration. The advantages of employing dual programming with FI-AFlFFF are demonstrated for sets of polystyrene sulfonate standards in the molecular mass range of 4 to 1000 kDa. It is shown that programmed FI-AFlFFF successfully expands the dynamic separation range of molecular mass. 相似文献
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The formation of Schiff bases from the reaction of primary amines and several aromatic aldehydes has been studied. In many cases the Schiff bases were too unstable or feebly-fluorescent to be of analytical value. 1-Pyrenealdehyde and 2-fluorenealdehyde, however, were found to be suitable fluorigenic reagents for primary aliphatic amines, forming Schiff bases that were very stable and intensely fluorescent in acidic ethanol. The derivatives of 1-pyrenealdehyde could be detected at concentrations less than 1 ng ml-1 in pure solution. Derivatives of 1-pyrenealdehyde could be readily produced by reactions at the surface of a t.l.c. plate. Combination of this approach with a simple deproteinizing procedure permitted analysis for nanograna quantities of primary amines in blood serum. 相似文献
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Hard form factors for meson-baryon strong couplings as derived from deep inelastic lepton scattering
W. -Y. P. Hwang J. Speth G. E. Brown 《Zeitschrift für Physik A Hadrons and Nuclei》1991,339(3):383-389
As stimulated by earlier attempts for obtaining theNN andN form factors from the deep inelastic lepton scattering data, we extend the analysis by taking into account effects of additional mesons including, , ,K, andK
*, with the coupling constants fixed by the lowenergy nucleon-nucleon and hyperon-nucleon scattering data. Contrary to an earlier claim that the NN andN form factor must be very soft (e.g., with the cutoff mass less than 500 MeV in the monopole form), we find, for example, that with all form factors parametrized in the dipole form, a universal cutoff mass of 1150 MeV in the/N sector and 1400 MeV in the/ sector yields predictions in excellent agreement with recently published neutrino data on the momentum fractions carried by thes, , and¯d quarks, as well as consistent with the sea-to-valence ratio extracted from the CDHS data and the Femilab E615 experiment. Similar results can also be obtained by using exponential cutoffs for all couplings, or by using monopole forms for some vertices while retaining dipole forms for the rest. The success of the mesonexchange picture in generating the strangeness content in a proton suggests an alternative understanding of the origin of sea quarks in the proton.We wish to thank G. Garvey, K. Holinde, L.-C. Liu, M. B. Johnson, M. Strikman, A. W. Thomas and Jochen Wambach for helpful conversations. W-Y. P. Hwang wishes to acknowledge the Alexander von Humboldt Foundation for a fellowship to visit Jülich for conducting research. His research works was also supported in part by the National Science Council of the Republic of China. The work of G. E. Brown is supported in part by a Humboldt award, in part by NATO Grant RG85/0093, and in part by the U.S. Department of Energy. 相似文献
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In this paper, we consider dense stars with configurations expected from the SU(3)C×SU(2)W× U(1) standard model of strong and electroweak interactions. Following a recent suggestion that strange matter, a form of (uds) quark matter, may be the true ground state of hadronic matter, we investigate the prospect for the existence of dense stars consisting partially, or entirely, of strange matter by comparing the relative stability between neutron matter and strange matter. It is found that the restriction on the maximum star mass holds in all cases, including a pure strange star, a pure neutron star, and a neutron star with a quark core. It is also found that the choice of both the bag constantB and the strong coupling constant s has a decisive effect on the relative stability between strange matter and neutron matter. For currently accepted values of (B, s), anA= dense starcannot consist entirely,nor partially, of strange matter. Nevertheless, such conclusion may be subject to change if corrections ofO (
s
2
) or other effects are taken into account. Finally, we use the framework of Tolman, Oppenheimer, and Volkoff to analyze two cases of boson stars: gluon stars and stars consisting of massive scalar particles (massive bosons). It is found that, in the case of gluon stars, the presence of the bag constant in the QCD vacuum yields results very similar to that found in quark stars. On the other hand, soliton stars consisting of massive bosons exist if there is some background pressure which plays the role similar to the bag constant for lowering the matter pressure. The stability problem for both gluon stars and soliton stars is briefly discussed. 相似文献