光谱法研究有机农药污染物与DNA的相互作用

利用紫外光谱、荧光光谱及圆二色谱(CD)等光谱方法研究了有机农药敌敌畏(DDVP)、乐果(DIM)、氧化乐果(OME)及生物农药申嗪霉素的有效成分吩嗪-1-羧酸(PCA)与端粒脱氧核糖核酸(DNA)间的相互作用。 结果表明,有机农药分子可能主要以嵌入模式与DNA结合,DDVP、DIM、OME以及PCA与端粒DNA之间的表观结合常数分别为：1.17×106、1.48×106、4.52×105和1.80×106 L/mol,与DNA结合能力的大小顺序为PCA＞DIM＞DDVP＞OME。 这些有机农药分子明显不利于DNA双螺旋结构的稳定性。 有机农药分子的嵌入削弱了DNA碱基对之间的π-π堆积作用,对DNA的二级结构有一定的影响,使得DNA的双螺旋结构变得松散。     

Infrared multiple photon dissociation action spectroscopy and computational studies of mass-selected gas-phase fluorescein and 2',7'-dichlorofluorescein ions

Fluorescein (FL) and its derivative 2',7'-dichlorofluoroescein (DCF) are well-known fluorescent dyes used in many biological and biochemical applications. Although extensive studies have been carried out to investigate their chemical and photophysical properties in different solvent media, little is known about their intrinsic behaviors in the gas phase. Here, infrared multiple photon dissociation (IRMPD) action spectra are reported for the three charged prototropic forms of FL and DCF and compared with computed IR spectra from electronic structure calculations. In each case, the measured spectra show good agreement with the calculated spectra of the lowest energy computed conformer. Moreover, the major bands of the monoanion IRMPD spectra show striking similarities to those of the dianions and are quite different from those of the cations. These experimental results clearly indicate that the gaseous monoanions are predominantly deprotonated on the xanthene chromophore, rather than the benzoate deprotonation site favored in solution. Investigations such as this, which provide a better understanding of intrinsic properties of ionic dyes, forms a baseline from which to elucidate solvent effects and will aid the rational design of dyes possessing desirable fluorescence properties.     

Procurement strategies for lost-sales inventory systems with all-units discounts

Despite the prevalence of all-units discounts in procurement contracts, these discounts pose a technical challenge to analyze procurement strategies due to neither concave nor convex ordering costs. In this paper, we consider the optimal procurement strategies with all-units discounts under the lost-sales setting. By assuming log-concave demands, we find that the optimal procurement strategies have a generalized Q-jump (s, S) structure by introducing a new notion of Q-jump single-crossing. In particular, a sufficient condition is provided for degenerating the optimal procurement strategies from a generalized Q-jump (s, S) structure into a Q-jump (s, S) structure, which is definitely optimal for the single-period problem. Extensive numerical results suggest that the Q-jump (s, S) policy as a heuristic performs considerably well when its optimality sufficient condition is violated. Our results can be extended to systems with multi-break all-units discounts, and systems with all-units discounts on batch ordering.     

Three-dimensional image reconstruction of an Hα flare on 26 March 2001

Three-dimensional (3D) image reconstruction of an Hα flare near the solar disk center, is done by a series of line-center and off-line-center filtering observation, according to the physical meaning of contribution function based on the line formation theory. The contribution function represents the contribution from stellar atmospheric layers to the emergent specific intensity and describes the depth distribution of escape photons. In this paper, an Hα flare of 26 March 2001 is used to illustrate the 3D line-of-sight reconstruction and its development     

正则Cosine算子函数的乘积扰动定理

本文研究了正则cosine算子函数的乘积扰动性,在正则化算子C的值域不 一定稠密的一般情形下,获得了若干正则cosine算子函数的乘积扰动定理.     

钩刺山羊草Aegilops triuncialis籽粒硬度主效基因的克隆与表达

根据小皮Pin a，Pin b，GSP基㈥的保守序列，设计合成了3对特异性引物，对四倍体钩刺山羊草Aegilopstriumialis的基因组DNA和胚乳RNA进行Pin a，Pin b，CSP基因扩增、克降、序列测定和表达分析，发现了两个新型Pin a等位堆因、一个新刑Pin b等化基因和一个新型GSP等位基因，基因序列均与六倍体小麦的同源基因存在较大的差异，Southern Blot分析结果表明，该材料巾含有2个拷贝Pin a。基因，5个拷贝Pin b基因，2个拷贝GSP基因．RT-PCR和Western Blot都证实了Pin a，Pin b，GSP基因在籽粒胚乳中的表达．研究结果显示由羊草可以为小麦分子育种提供有用的遗传资源。     

Study on the constitutive equation with fractional derivative for the viscoelastic fluids – Modified Jeffreys model and its application

Based on the classic linear viscoelastic Jeffreys model, a modified Jeffreys model is suggested. The corresponding five-parameter equation with fractional derivatives of different orders of the stress and rate of strain is stated and the characteristic material functions of the linear viscoelasticity theory, such as the dynamic moduli, are derived. The comparison between the measured dynamic moduli of Sesbania gel and xanthan gum and the theoretical predictions of the proposed phenomenological model shows an excellent agreement. Received: 26 August 1997 Accepted: 26 May 1998     

Hallkoeffizient,spezifischer elektrischer Widerstand und magnetische Suszeptibilität flüssiger Hg-In-und Ga-In-Legierungen

The Hall coefficient and the magnetic susceptibility of the liquid alloys Hg-In and Ga-In have been measured and compared with the free electron model. The deviation of the measured from the calculated Hall coefficient for Hg-In is discussed. The susceptibility of carriers in Hg and Hg-In deviates strongly from the Pauli-Landau-susceptibility. This behaviour can be understood by assuming a minimum of the density of states at the Fermi level of Hg. The susceptibility data accounts for the Knight-shift ofSeymour andStyles. For liquid Ga-In, the Hall-coefficient and the magnetic susceptibility agree with the free electron model.     

Bilayer lipid membranes formed from thermotropic liquid crystals

This paper reports a new type of bilayer lipid membrane (BLM) with aqueous interfaces which are formed from thermotropic liquid crystals (nematic-6CB, smectic-8CB, and cholesteric-cholesteryl palmitate, ChP). The electrical properties of these unmodified membranes have been investigated. We suggest that BLMs of this type in their 'black' states consist of two molecular layers with a smectic-like structure.