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101.
复制缺陷型人泡沫病毒载体辅助质粒pΔGP的构建及转染小肠癌细胞的研究 总被引:1,自引:1,他引:0
在人泡沫病毒原病毒全长克隆pHRSV13的基础上,缺失突变gag和pol基因,并且用SV40polyA加尾信号替代人泡沫病毒的3′LTR,构建辅助质粒pΔGP.将复制缺陷型人泡沫病毒载体质粒pGPSNI EGFP和辅助质粒pΔGP分别转染和共转染小肠癌HIC细胞系,荧光显微镜检测发现共转染pGPSNI EGFP和pΔGP的HIC细胞能够强烈表达绿色荧光蛋白,转染有复制缺陷型人泡沫病毒载体质粒pGPSNI EGFP的HIC细胞能够表达少量的绿色荧光蛋白,而转染有辅助载体pΔGP的HIC细胞不表达绿色荧光.结果证明复制缺陷型人泡沫病毒载体的构建成功,表明人泡沫病毒env基因3′端的内部启动子IP具有弱启动子的活性,并且bel基因产生的调控蛋白能够反式激活人泡沫病毒内部启动子IP和5′LTR的启动子. 相似文献
102.
This paper seeks to solve the difficult nonlinear problem in financial markets on the complex system theory and the nonlinear
dynamics principle, with the data-model-concept-practice issue-oriented reconstruction of the phase space by the high frequency
trade data. In theory, we have achieved the differentiable manifold geometry configuration, discovered the Yang-Mills functional
in financial markets, obtained a meaningful conserved quantity through corresponding space-time non-Abel localization gauge
symmetry transformation, and derived the financial solitons, which shows that there is a strict symmetry between manifold
fiber bundle and guage field in financial markets. In practical applications of financial markets, we have repeatedly carried
out experimental tests in a fluctuant evolvement, directly simulating and validating the existence of solitons by researching
the price fluctuations (society phenomena) using the same methods and criterion as in natural science and in actual trade
to test the stock Guangzhou Proprietary and the futures Fuel Oil in China. The results demonstrate that the financial solitons
discovered indicates that there is a kind of new substance and form of energy existing in financial trade markets, which likely
indicates a new science paradigm in the economy and society domains beyond physics.
相似文献
103.
104.
Xiao‐Hui Liu Yan‐Guo Li Ying Lin Yue‐Sheng Li 《Journal of polymer science. Part A, Polymer chemistry》2007,45(7):1272-1281
The reversible addition–fragmentation chain transfer (RAFT) polymerization of acrylonitrile (AN) mediated by 2‐cyanoprop‐2‐yl dithiobenzoate was first applied to synthesize polyacrylonitrile (PAN) with a high molecular weight up to 32,800 and a polydispersity index as low as 1.29. The key to success was ascribed to the optimization of the experimental conditions to increase the fragmentation reaction efficiency of the intermediate radical. In accordance with the atom transfer radical polymerization of AN, ethylene carbonate was also a better solvent candidate for providing higher controlled/living RAFT polymerization behaviors than dimethylformamide and dimethyl sulfoxide. The various experimental parameters, including the temperature, the molar ratio of dithiobenzoate to the initiator, the molar ratio of the monomer to dithiobenzoate, the monomer concentration, and the addition of the comonomer, were varied to improve the control of the molecular weight and polydispersity index. The molecular weights of PANs were validated by gel permeation chromatography along with a universal calibration procedure and intrinsic viscosity measurements. 1H NMR analysis confirmed the high chain‐end functionality of the resultant polymers. © 2007 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 45: 1272–1281, 2007 相似文献
105.
106.
By means of improved ligand-field theory, the "pure electronic" pressure-induced shifts (PS's) and the PS's due to electron-phonon interaction (EPI) of R1 line and R2 line of YAG:Cr3 have been calculated, respectively.The calculated results are in very good agreement with the experimental data. It is demonstrated that the admixture of |t22(3T1)e4T2> and |t322E> bases in the wavefunction of R1 level of YAG:Cr3 and its change with pressure play a key role for the PS of R1 line. The behaviors of the "pure electronic" PS of R1 line and the PS of R1 line due to EPI are different. It is the combined effect of them that gives rise to the total PS of R1 line, which has satisfactorily explained the experimental results. The systematic analyses and comparisons between the feature of R1-line PS of YAG:Cr3 and the ones of three laser crystals (GSGG:Cr3 , GGG:Cr3 and ruby) have been made, and the origin of the difference between them has been revealed. 相似文献
107.
108.
Modulation of Spin Distribution and Spin Transport by a Magnetic Field in a Quasi-One-Dimensional System with Spin--Orbit Coupling
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The distributions of spin and currents modulated by magnetic field in a transverse parabolic confined two-dimensional electronic system with a Rashba spin--orbit coupling have been studied numerically. It is shown that the spin accumulation and the spin related current are generated by magnetic field if the spin--orbit coupling is presented. The distributions of charge and spin currents are antisymmetrical along the cross-section of confined system. A transversely applied electric field does not influence the characteristic behaviour of charge- and spin-dependent properties. 相似文献
109.
氧化锆材料是近年来备受关注的陶瓷材料之一.它在许多不同的领域,诸如陶瓷颜料、工程陶瓷、宝石业、压电元件、离子交换器以及固体电解质方面等有着广泛的用途[1].其最常用的性质是其晶相转变时会有效改善陶瓷的脆性,因此在室温下保持其四方相是其中的关键.为此许多研究都侧重在其中加入各种添加剂,利用水热法、包裹法、形成共溶体等方法来阻止其晶格的转化[2-4].但研究也发现:在固定组成陶瓷基体中,ZrO2的相变温度随粉体颗粒直径的减小而降低,当颗粒足够小时能够提高材料强度的四方ZrO2可以保存到室温甚至室温以下.因此,减小ZrO2粉体粒度对于提高材料强度是非常有利的.本实验采用一种新的低温固相反应的方法制备氧化锆超细粉,从而使这种重要的陶瓷原料的制备有了新的思路. 相似文献
110.
��˴������ Ⱥ���ӷ��� 《核聚变与等离子体物理》2006,38(4):386-393
The high speed CCD visible-light camera system has been installed to detect the dynamic characteristics of ELMs filaments on EAST tokamak. In order to convert the CCD video imaging coordinates into real space coordinates, the CCD system has calibrated using Tsai’s two-stage calibration technique. A raised spike in the grayscale profile, drawn by selecting the grayscale value of the image in the vertical direction of the ELMs filament in the CCD image, represents an ELMs filament structure whose positional coordinates are determined by the apex of the spike. The Sobel edge detection operator was used to examine the edge profile of the filament to obtain the width of the filament. By analyzing the characteristics of the filament width of the ELMs, it is found that the width of ELMs filaments continuously decreases outward from the outermost magnetic surface. This indicates that the filaments continue to dissipate energy and particles as they propagate outward. The width of filaments of Type-I ELMs is greater than that of Type-III ELMs. 相似文献