Purification of a hybrid plasminogen activator protein

Recombinant DNA technology has been employed to produce a hybrid gene in which the kringle and serine protease domains of tissue plasminogen activator are linked to the heavy-chain Fd region of a fibrin-specific antibody. The hybrid gene is co-expressed with antibody light chains. This communication describes a purification procedure for the hybrid protein, involving affinity and ion-exchange chromatography. The purified hybrid protein has been used in vivo and in vitro clot lysis experiments and has been shown to be effective at clot dissolution.     

Coupled cavity modelocking of a Nd:YAG laser using second-harmonic generation

The modelocking of a Nd:YAG laser using second-harmonic generation in an external cavity as the only pulse shortening process is reported. The output of a long pulse Nd:YAG laser was simultaneously modelocked and Q-switched. The average pulse duration was 30–50 ps and the peak power was in the range 60–160 kW.     

Forecasting practice: organisational issues

Although many surveys have been conducted on forecasting practice, the majority of surveys have investigated the utilisation of forecasting methods and relatively few have studied the role of forecasting in changing organisational structures. The aim of this paper is to address this issue by expanding on earlier case studies which suggested the need for a re-location of the forecasting function within the organisation. Current research has shown how the role of forecasting has changed as organisations adapt to meet the business needs of their customers. The evidence reported in this paper was gathered by surveys and structured interviews carried out in the electronics and financial industries in Scotland.     

1H‐Pyrrole‐2‐carbothioamide

The reaction of ethoxy­carbonyl­pyrrole‐2‐thio­carbox­amide with sodium hydro­xide affords the known title compound, C₅H₆N₂S, whose structure shows a three‐dimensional hydrogen‐bonded lattice.     

μ‐Oxo‐bis[(η5‐1,2‐dicarbaundecabor­ato)bis(N,N‐dimethylacetamidinato)tantalum] dichloromethane hemisolvate

The structure of the title compound, [Ta₂O(C₂B₉H₁₁)₂(C₄H₉­N₂)₄]·0.5CH₂Cl₂, contains two (C₂B₉H₁₁)Ta[NC(Me)NMe₂]₂ units bridged by a nearly linear [Ta—O—Ta 163.4 (4)°] μ‐oxo ligand. The dichloromethane molecule lies on a twofold axis.