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31.
Susana Rocha Dr. James A. Hutchison Dr. Kalina Peneva Dr. Andreas Herrmann Prof. Dr. Klaus Müllen Prof. Dr. Michael Skjøt Dr. Christian I. Jørgensen Dr. Allan Svendsen Dr. Frans C. De Schryver Prof. Dr. Johan Hofkens Prof. Dr. Hiroshi Uji‐i Dr. 《Chemphyschem》2009,10(1):151-161
Many of the biological processes taking place in cells are mediated by enzymatic reactions occurring in the cell membrane. Understanding interfacial enzymatic catalysis is therefore crucial to the understanding of cellular function. Unfortunately, a full picture of the overall mechanism of interfacial enzymatic catalysis, and particularly the important diffusion processes therein, remains unresolved. Herein we demonstrate that single‐molecule wide‐field fluorescence microscopy can yield important new information on these processes. We image phospholipase enzymes acting upon bilayers of their natural phospholipid substrate, tracking the diffusion of thousands of individual enzymes while simultaneously visualising local structural changes to the substrate layer. We study several enzyme types with different affinities and catalytic activities towards the substrate. Analysis of the trajectories of each enzyme type allows us successfully to correlate the mobility of phospholipase with its catalytic activity at the substrate. The methods introduced herein represent a promising new approach to the study of interfacial/heterogeneous catalysis systems. 相似文献
32.
Merzlyakov M Li E Casas R Hristova K 《Langmuir : the ACS journal of surfaces and colloids》2006,22(16):6986-6992
F?rster resonance energy transfer (FRET), a fluorescence detection technique, is often used for sensing molecular interactions in solution and in membranes. Here we show that (1) FRET spectra can be recorded in single bilayers, supported on a surface, and (2) the fluorescein/rhodamine dye pair is an adequate reporter of FRET when spectral detection is used. Thus, measurements pertaining to molecular interactions in membranes can be carried out in supported bilayers. Spectral FRET has advantages over imaging FRET, which monitors only signal amplitudes at certain wavelength. There are also advantages to performing spectral FRET measurements in supported bilayers as compared to free liposomes in suspension. However, the spectral properties of dyes can be altered in an unexpected manner in an ordered bilayer structure on a surface, such that fluorescence detection in surface-supported bilayers is not always trivial. 相似文献
33.
Moving freely: A recent model for voltage gating of potassium channels proposed that the four arginine residues of the voltage-sensing S4 helix (left) are in direct contact with the membrane lipids and move into the hydrocarbon core of the membrane during gating. It is demonstrated that the physical properties of the isolated S4 sequence (right) are sufficient to allow it to freely translocate across synthetic membranes. 相似文献
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Maya Marinova Kalina Kostova Pavleta Tzvetkova Maya Tavlinova-Kirilova Angel Chimov Rosica Nikolova Boris Shivachev Vladimir Dimitrov 《Tetrahedron: Asymmetry》2013,24(23):1453-1466
Dihydroxy naphthalenes have been applied in Betti-type solventless condensation between aldehydes and (S)-phenylethylamine as a chiral auxiliary. A series of chiral 1,3-aminonaphthols has been synthesized and isolated in diastereoisomerically pure form. The absolute configurations of the aminonaphthols synthesized have been determined by means of advanced NMR experiments and confirmed by X-ray crystallography. The new chiral aminonaphthols have been tested as pre-catalysts for the addition of diethyl zinc and alkynyl-Zn-reagents to aldehydes with enantioselectivities of up to 98% ee. 相似文献
37.
Syntheses of Macrocyclic Lactones by Ring Enlargement Reaction A general method is given for the synthesis of macrocyclic lactones by ring enlargement of 2-nitrocycloalkanones by four ring members. By this procedure 2-nitrocyclooctanone ( 1 ) is transformed to 8-oxo-dodecan-11-olid ( 5 ) in an overall yield of 63,5% and 2-nitrocyclododecanone to 12-oxo-hexadecan-15-olid with 60% yield. 相似文献
38.
Peneva K Mihov G Herrmann A Zarrabi N Börsch M Duncan TM Müllen K 《Journal of the American Chemical Society》2008,130(16):5398-5399
Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon binding of the nickel ions. Herein we present the solution and solid phase synthesis of water-soluble perylene(dicarboximide) functionalized with a nitrilotriacetic acid moiety (PDI-NTA). The photophysical properties of PDI-NTA revealed an exceptional photostability and fluorescence quantum yield that remained unchanged upon addition of nickel ions. The F1 complex of F0F1-ATP synthase from Escherichia coli, containing three hexahistidine tags, was labeled and the suitability for site-specific labeling of the new chromophore demonstrated using fluorescence correlation spectroscopy. 相似文献
39.
Tang T Herrmann A Peneva K Müllen K Webber SE 《Langmuir : the ACS journal of surfaces and colloids》2007,23(8):4623-4628
Multilayer films of water-soluble anionic and cationic perylene diimide (PDI) moieties have been prepared using the molecular layer-by-layer method described in an earlier publication (Tang, T. J.; Qu, J. Q.; Müllen, K.; Webber, S. E. Langmuir 2006, 22, 26-28) and the fluorescence intensity compared with and without a base layer prepared using an anionic terrylene diimide dye (n-TDI), which serves as an energy-trapping layer for the PDI exciton. The fluorescence quenching data could be fit equally well to a modification of a model used by Kuhn to describe energy transfer from a J aggregate or a model developed by Kenkre and Wong to describe excitonic transfer. For both models, we obtain a characteristic energy-transfer distance on the order of 5.4 nm. Fluorescence quenching of the PDI via a single F?rster energy-transfer step to the n-TDI layer is ruled out on the basis of the observed power-law dependence. We also consider a model in which the excitation is trapped at the outermost surface. This model provides a reasonable fit to the data only if the Kuhn relationship is used. 相似文献
40.
Ravi Agarwal D. O’Regan S. Hristova 《Journal of Applied Mathematics and Computing》2017,53(1-2):147-168
The stability of the solutions of a nonlinear differential equation with noninstantaneous impulses is studied using Lyapunov like functions. In these differential equation we have impulses, which start abruptly at some points and their action continue on given finite intervals. Sufficient conditions for stability, uniform stability and asymptotic uniform stability of the solutions are established. Examples are given to illustrate the results. Also, some of the results are applied to study a dynamical model in Pharmacokinetics. 相似文献