Observing three-dimensional human microvascular and myogenic architecture using conventional fluorescence microscopy

Microangiography and vascular casting have previously been used to demonstrate the three-dimensional architecture of human uterine microvasculature. However, a limitation of these perfusion-dependent techniques is the difficulty in identifying surrounding tissue components. We have previously shown that it is possible to visualise microvascular networks on the cut surfaces of fresh tissue specimens by diffusive labelling of vascular endothelium with fluorescently conjugated UEA-1 lectin. Unlike perfusion methods that are limited to accessible vascular networks, diffusive fluorescence labelling (DFL) allows additional visualisation of extravascular cellular components, such as smooth muscle. Following UEA-1 DFL, smooth muscle-myosin and -actin were then visualised by immunolocalisation on the acetone-fixed tissue pieces. This allowed clear three-dimensional distinction between the vascular and muscle architecture of the myometrium and endometrium. This method can also be applied for studying the relative distribution of microvascular and muscle architecture in leiomyomas (fibroids). The techniques described in this methodological study provide a simple way of directly examining the uterine vasculature in three dimensions using conventional microscopy, while also distinguishing myometrial from endometrial parts of the network.     

Compensation of self-phase modulation in fiber-based chirped-pulse amplification systems

We demonstrate a simple, all-fiber technique for removing nonlinear phase due to self-phase modulation in fiber-based chirped-pulse amplification (CPA) systems. Using a LiNbO3 electro-optic phase modulator to emulate a negative nonlinear index of refraction, we are able to remove 1.0 pi rad of self-phase modulation acquired by pulses during amplification and eliminate nearly all pulse distortion. Our technique is high speed, removes nonlinear phase on a pulse-to-pulse basis, and can be readily integrated into existing CPA systems.     

Small-angle neutron scattering study of concentrated colloidal dispersions: the electrostatic/steric composite interactions between colloidal particles

Small-angle neutron scattering was used to investigate the interactions in concentrated colloidal dispersions containing silica or polystyrene latex with adsorbed polyethyleneoxide (PEO). In these dispersions of charged particles, both electrostatic and steric repulsions are present. The PEO layer was made invisible to neutrons through contrast matching. The effect of the interparticle repulsion was clearly shown in the scattering spectra by the appearance of a peak at low Q. The effective potentials can be well described by the Hayter-Penfold/Yukawa (HPY) potential. In the silica dispersions studied, the layer thickness is small, hence the electrostatic potential dominates and the potential has a lower concentration dependence. In the dispersions of polystyrene latex, the adsorbed layer is thicker; consequently, the electrostatic potential dominates at low volume fraction (the potential has a lower concentration dependence), and the steric potential dominates at higher volume fraction (the potential has a higher concentration dependence). This study also suggests that when more than one potential is present the stronger one has a dominant influence in determining the structure factor. This finding makes it possible to describe the multicomponential interactions by a single function.     

Controlled association in suspensions of charged nanoparticles with a weak polyelectrolyte

The properties of high-pH suspensions of mixtures of silica with low-molecular-weight samples of the water-soluble polymer polyethylenimine (PEI) have been studied. At pH > 10 and low ionic strength, silica nanoparticles are stabilized by a negative surface charge, and PEI has only a very low positive charge. The adsorption of PEI induces a localized positive charge on the segments of polymer closest to the silica surface. The parts of the molecule furthest away from the surface have little charge because of the high pH of the medium. The polymer-covered particle remains negatively charged, imparting some electrostatic stabilization. Suspensions of silica and low-molecular-weight PEI are low-viscosity fluids immediately after mixing, but aggregation occurs leading to the eventual gelation (or sedimentation at lower concentrations) of these mixtures, indicating colloidal instability. The gelation time passes through a minimum with increasing surface coverage. The rate of gelation increases exponentially with molecular weight: for molecular weight > or = 10,000 Da PEI, the instability is so severe that uniform suspensions cannot be produced using simple mixing techniques. The gelation rates increase rapidly with temperature, ionic strength, and reduction in pH. The rate of gelation increases with increasing particle concentration at low surface coverage but decreases at high coverage as a consequence of a small increase in pH. Gels are broken by application of high shear into aggregates that re-gel more rapidly than the original discrete coated particles.     

Controlled, simultaneous assembly of polyethylenimine onto nanoparticle silica colloids

A novel precision-assembly methodology is described on the basis of the controlled, simultaneous assembly (CSA) of a core nanoparticle substrate and polyelectrolyte solutions. The method is capable of assembly rates at least as fast as 10(16) core particles s(-1) L(-1) and affords concentrated suspensions of stable colloids with an adsorbed polyelectrolyte. The resulting dispersions are highly homogeneous, have a low viscosity and narrow particle-size distribution, and are stable colloids, even at solid concentrations of at least 33 wt %. The adsorption isotherm and the saturation adsorption for polyethylenimine (PEI) assemblies onto a 15 nm silica colloid have been evaluated with 1H NMR spectroscopy. The saturation adsorption is highly dependent upon the pH at assembly and is given by the equation PEIa (micromol m(-2)) = 1.73pH - 1.89, R2 = 0.986, where micromoles refers to the concentration of the EI monomer. The saturation concentration increases from 6.8 micromol m(-2) at pH 5.0 to 13.7 micromol m(-2) at pH 9.0. The adsorbed polyelectrolyte may be cross-linked and thereby permanently fixed to the colloid surface to prepare nanoparticle-polyelectrolyte colloidal assemblies having enhanced colloid stability, high homogeneity, and a high fraction (>80%) of permanently adsorbed polyelectrolyte. These assemblies are stable at physiological pH and ionic strength and may represent ideal substrates for bioconjugation and, ultimately, the design of nanocarriers for in vivo applications.     

A novel transformation of a zirconium imido compound and the development of a new class of N3 donor heteroscorpionate ligand

Reaction of the dimeric zirconium imido compound [Zr2(mu-NAr)2Cl4(THF)4] with tris(3,5-dimethylpyrazolyl)methyl silane very selectively gave [Zr{(Me2pz)2Si(Me)NAr}Cl3] (1), a highly active pre-catalyst for ethylene polymerisation; a more general and versatile route to N3 donor heteroscorpionate compounds was achieved via the protio ligand (Me2pz)2CHSi(Me)2N(H)iPr for which neutral and cationic organometallic Group 3 and 4 derivatives are reported (Ar = 2,6-C6H(3)iPr2).     

On sound generated by gas-jet impingement on a bubbly gas–water interface,with application to supercavity self-noise

An analysis is made of the sound produced when a high speed turbulent gas jet impinges at normal incidence on a planar gas–water interface in the presence of a uniform, thin homogeneous bubble layer between the gas and the water. It is shown that the bubble layer exhibits a behaviour similar to a ‘quarter-wave’ resonator, storing energy supplied by gas impingement which is subsequently released as high amplitude sound into the water. In the absence of bubbles the radiation into the water has dipole characteristics, peaking strongly in the direction normal to the interface. The bubbles diffuse this sharp dipole lobe, and are predicted to increase the sound power in the water by up to 10 dB or more over a range of intermediate frequencies when the layer properties are similar to those encountered in experiments using a model scale supercavitating vehicle. At higher frequencies, in the range important for vehicle guidance and control, the bubble layer tends to reduce this source of self-noise. The same mechanism of resonant amplification should be effective more generally, when the bubbles lie within a more confined surface envelope, such as the surface ‘wake’ of the jet impact zone, whose shape defines a different set of interior eigenmodes.