Computational insight into asymmetric uranyl‐salophen coordinated with α, β‐unsaturated aldehydes and ketones

The study of the catalytic activity and activation mechanism of asymmetric uranyl‐salophens with α, β‐unsaturated aldehydes or α, β‐unsaturated ketones, is a research hotspot. In this paper, the complexes of the uranyl–salophen(U‐S) modified by unilateral benzene, coordinated with cyclohexenone, cyclopentenone and acrolein, were investigated using density functional theory calculations at the level of B3LYP/6‐311G(d, p) basis set. The results showed that the uranyl‐salophen(U‐S) weakened the large π bond between C = C and C = O of the α, β‐unsaturated aldehydes and ketones, making the unsaturated aldehydes and ketones activated. In addition, the molecular‐recognition selectivity of the asymmetrical uranyl‐salophen for cyclohexenone and cyclopentenone were much higher than for acrolein.     

Combined use of ionic liquid and β‐CD for enantioseparation of 12 pharmaceuticals using CE

In this study, the enantioseparation of zopiclone, repaglinide, chlorphenamine maleate, brompheniramine maleate, dioxopromethazine hydrochloride, promethazine hydrochloride, liarozole, carvedilol, homatropine hydrobromide, homatropine methylbromide, venlafaxine, and sibutramine hydrochloride has been investigated using β‐CD in combination with a chiral ionic liquid (IL), 1‐ethyl‐3‐methylimidazolium‐L‐lactate. The influence of the type of IL and its concentration, BGE pH, and chain length of the IL cations on the resolution are discussed. Finally, the proposed method was successfully applied for the chiral impurity determination of eszopiclone in pharmaceutical tablets after validation with respect to accuracy and precision, linearity range, selectivity, repeatability, LOD and LOQ. It is assessed that the chiral impurity determination in the commercial tables is fewer than 0.1%.     

Quantitative study of cellular heterogeneity in doxorubicin uptake and its pharmacological effect on cancer cells

Cellular heterogeneity in doxorubicin (DOX) uptake and its relationship with pharmacological effect on cancer cells were quantitatively investigated for the first time. An in vitro experimental model was established by treating human leukemia K562 and breast cancer MCF‐7 cells with different schedules of DOX with or without surface P‐glycoprotein (P‐gp) inhibitor verapamil (VER). The cellular heterogeneity in DOX uptake was quantitatively examined by single‐cell analysis using capillary electrophoresis coupled with laser‐induced fluorescence detection. The corresponding cytotoxic effect was tested by cellular morphology, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium and flow cytometry assays. The expression of cellular membrane surface P‐gp was determined by flow cytometry. Results showed that the cellular heterogeneity exists in DOX uptake. The single‐high DOX schedule leads to lower uptake heterogeneity and higher mean drug uptake. The cellular heterogeneity in DOX uptake was found to be negatively correlated with drug cytotoxicity and surface P‐gp expression, with r = ?0.7680 to ~ ?0.9587. VER reduces the cellular variation in DOX uptake, suggesting that surface P‐gp may be one of the causes of the cellular heterogeneity in DOX uptake. This research demonstrates the importance of quantitative study of cellular heterogeneity in drug uptake and its potential application in drug schedule design, response prediction and therapy modulation. Copyright © 2014 John Wiley & Sons, Ltd.     

Minireview: Advances in Germanium Isotope Analysis by Multiple Collector–Inductively Coupled Plasma–Mass Spectrometry

The advent of multiple collector–inductively coupled plasma–mass spectrometry (MC-ICP-MS) has made the high-precision determination of Ge isotopes possible, which leads to the widespread application of Ge isotopes in earth, ocean, and cosmochemistry fields. This paper reviews the history of Ge isotope analysis, chemical dissolution and purification, and mass spectrometry measurements. Concentrated HNO₃ is sufficient to dissolve nearly all types of samples and HF is also involved for Si-rich samples. Low-temperature ashing prior to dissolution is an alternative way to preconcentrate Ge in organic-rich samples. For different matrices, Ge isotopes can be determined by MC-ICP-MS coupled with a traditional nebulizer system or hydride generation system after two-step separation, one step cation/anion-exchange separation, or Mg/Fe co-precipitation protocols. Ion-exchange column methods are suitable for samples with elevated matrix and Ge content such as sulfides, iron oxides, silicate rocks, and coals, whereas Mg or Fe coprecipitation methods are particularly suitable for all kinds of water. Hydride generation systems are improved over traditional nebulizer system due to the smaller sample quantity and fewer matrix-related interferences. Sample-standard bracketing, double spike, and external Ga isotope normalization are used to mass bias correction and yield consistent results. Analytical methods involving Ge-poor samples and Ge isotope analyses based on different Ge species or specific Ge compound in natural environment will be important prospects in the further study. For further applications of Ge isotopes in mineral deposits such as sulfide and iron oxide deposits, sulfides, and iron oxides reference materials should be developed in the future.     

A class of finite groups with complete character degree graphs

In this paper, we construct a new class of finite groups whose common divisor graphs are complete graphs, while there is no prime dividing all the nontrivial degrees.     

Synthesis of Novel Jusbetonin Analogues and Their Cytotoxicity against Tumor Cell

Six novel analogues of bioactive natural indolo[3,2‐b]quinoline alkaloid glycoside jusbetonin were designed and synthesized, employing polyphosphoric acid mediated cyclization and two different amidation strategies on introduction of tetra‐O‐acetyl‐β‐d ‐glucosamine to tetracyclic carboxylic acids.     

Multiresidue determination of 29 pesticide residues in pepper through a modified QuEChERS method and gas chromatography–mass spectrometry

This study describes the development and use of a modified quick, easy, cheap, effective, rugged and safe (QuEChERS) method coupled with gas chromatography with mass spectrometry to determine 29 pesticide residues in green, red and dehydrated red peppers. Pesticides were extracted with acetonitrile (1% acetic acid), partitioned with sodium chloride and purified with primary secondary amino and octadecyl silane in acetone. The QuEChERS extraction conditions were optimized, and the matrix effects that might influence recoveries were evaluated and minimized using matrix‐matched calibration curves. Under the optimized conditions, the calibration curves for 29 pesticides showed good linearity in the concentration range of 0.1–10 μg/mL with determination coefficient R² > 0.998. The limits of quantification of the 29 pesticides were 0.006–0.06 mg/kg for green pepper, 0.005–0.039 mg/kg for red pepper and 0.014–0.25 mg/kg for dehydrated red pepper. These values are below the suggested regulatory maximum residue limits. The mean recoveries ranged between 70.1 and 110%, and the relative standard deviations were <13%. The developed method was successfully applied to commercial samples. Some samples were found to contain the 29 pesticides with levels below the legal limits. Copyright © 2016 John Wiley & Sons, Ltd.     

Solution-processable graphenes by covalent functionalization of graphene oxide with polymeric monoamines

We develop here a simple wet chemistry to prepare covalent functionalized graphenes(FGs) through epoxide aminolysis especially under alkaline aqueous condition. Remarkably, a series of typical monoamines, such as industrial Huntsman Jeffamine~ M-2070 and M-2005 polymer with hydrophilic or hydrophobic polyetheramine chains, positively-charged 2-amino-N,N,Ntrimethylpropanaminium,negatively-charged sulfanilic acid, even oligopeptide sequence, can be effectively grafted on the platelets of graphene oxide precursor with covalent functionalization and partially reduced features. This strategy provides the researchers a facile and convenient approach to design and synthesize solution processable, biocompatible and functionalized graphenes for the potent applications in electronic inks, drug carriers and biomedicines. Expansion of the current study is actively ongoing in our laboratory.