基于可靠性模型及数据融合的冷却风扇健康管理算法

针对目前工业领域对冷却风扇的故障预测与健康管理的需求,提出了基于可靠性模型及信息融合的冷却风扇健康管理算法;文中对风扇的主要性能参数进行辨识,包括转速、电流、上升时间、下降时间、温度以及适度,结合风扇故障模型,将冷却风扇健康管理算法分为环境、机械及电子3个模块;利用可靠性模型及信息融合,对风扇状态进行评估以及预测,进而对风扇的剩余寿命及故障进行预测。     

CuCoMn-zeolite双功能催化剂转化生物质基合成气制取液态烃

选用四种不同的分子筛(SAPO-34, ZSM-5, Y, MCM-41)与CuCoMn(高醇合成组元)构成双功能催化剂,利用N₂吸脱附、H₂-TPR、XRD、NH₃-TPD等表征了催化剂的结构性质. 研究了催化剂在生物质基合成气一段法制取液态烃燃料的应用. 相比于CuCoMn催化剂,加入分子筛的双功能催化剂均不同程度地提高了液体烃燃料的选择性及收率,且收率按顺序递减呈CCM-ZSM-5>CCM-SAPO-34>CCM-Y>CCM-MCM-41. 同时,共沉淀法制备的CuCoMn-ZSM-5 (20wt%, Si/Al=100) 具有最佳的CO转化率(76%)及液体产物收率(30%). 相比于CuCoMn氧化物,双功能催化剂的比表面及孔容均得到提高. CCM-ZSM-5具有适中的微孔尺寸和中等强度的酸性,增加CCM-ZSM-5中ZSM-5含量或降低ZSM-5中的Si/Al比,均有利于提高酸性位的数量,主要是较弱的酸性位. 而共沉淀法制备的CCM-ZSM-5具有更好的金属分散性及还原性能.     

石英晶体微天平用于微囊藻毒素在聚合物涂覆的金片表面吸附

合成了具有不同功能化的聚苯乙烯树脂,利用石英晶体微天平对树脂和微囊藻毒素相互吸附作用进行实时监测和研究．研究发现,吸附的pH和表面性质对于吸附量有重要的影响,而温度影响不大,中性pH氨基树脂对微囊藻毒素的吸附效果最好     

Estimation of pulsed laser-induced single event transient in a partially depleted silicon-on-insulator 0.18-μm MOSFET

In this paper, we investigate the single event transient(SET) occurring in partially depleted silicon-on-insulator(PDSOI) metal–oxide–semiconductor(MOS) devices irradiated by pulsed laser beams. Transient signal characteristics of a 0.18-μm single MOS device, such as SET pulse width, pulse maximum, and collected charge, are measured and analyzed at wafer level. We analyze in detail the influences of supply voltage and pulse energy on the SET characteristics of the device under test(DUT). The dependences of SET characteristics on drain-induced barrier lowering(DIBL) and the parasitic bipolar junction transistor(PBJT) are also discussed. These results provide a guide for radiation-hardened deep sub-micrometer PDSOI technology for space electronics applications.     

Application of Dispersive Liquid�CLiquid Microextraction for the Analysis of Six Fungicides in Fruit Samples by GC�CECD

A novel approach for the determination of six fungicides (triadimefon, procymidone, hexaconazole, myclobutanil, diniconazole and iprodione) in fruit samples is presented. Analytes were extracted using the dispersive liquid?Cliquid microextraction technique and determined by GC?CECD. Parameters affecting the dispersive liquid?Cliquid microextraction performance, such as the kind and volume of extraction and dispersive solvents, extraction time and salt concentration, were studied and optimized. Under the optimum extraction conditions, the linearities of the method were obtained in the range of 0.5?C20.0 ??g kg^?1 for triadimefon, hexaconazole, diniconazole and procymidone, and 1.0?C40.0 ??g kg^?1 for myclobutanil and iprodione, with the correlation coefficients ranging from 0.9902 to 0.9995. The enrichment factors ranged from 685 to 820 and the extraction recoveries ranged from 81.3 to 98.4%. The relative standard deviations varied from 3.1 to 7.8%. The limits of detection of the method were in the range of 0.02?C0.12 ??g kg^?1. Results showed that the method we proposed can meet the requirements for the determination of target fungicides in fruit samples. Several compounds considered in this study were found in fruit samples.     

Three-Phase Solvent Bar Microextraction Combined with HPLC for Extraction and Determination of Plasma Protein Binding of Bisoprolol

A three-phase solvent bar microextraction (TPSBME) technique combined with high performance liquid chromatography (HPLC)?Cfluorescence detection was evaluated for the quantitative determination of plasma protein binding of bisoprolol. Bisoprolol was extracted from a 5.6-mL basified plasma sample (donor phase) into the organic solvent (n-octanol) impregnated in the pores of a hollow fiber and then extracted into an acidic solution (acceptor phase) inside the lumen of the hollow fiber. Metoprolol was used as the internal standard. Several parameters influencing the efficiency of the method were investigated and optimized including organic solvent (n-butanol, n-octanol, dibutyl phthalate, dihexyl ether), stirring rate (100?C1,000 rpm), extraction time (5?C35 min), extraction temperature (15?C45 °C), concentration of the donor phase (0.1?C2 M NaOH) and the acceptor phase (0.5?C5 M formic acid), salt concentration (2.5?C10%, w/v). Under the optimal condition, extraction recoveries from plasma samples were above 61.4% for bisoprolol. The calibration curves were obtained in the range of 10?C100 ng mL^?1 with reasonable linearity (r > 0.994). The method was successfully applied to determine the plasma protein binding rate of bisoprolol.     

Determination of SYUIQ-F5, a Novel Telomerase Inhibitor and Anti-tumor Lead-Compound, in Tissues and Plasma Samples by LC�CMS�CMS: Application to a Tissue Distribution Study in Rat

A sensitive assay for determining SYUIQ-F5, a novel telomerase inhibitor and anti-tumor drug, in rat tissues and plasma was developed and validated by using liquid chromatography/tandem mass spectrometry (LC?CMS?CMS). After a single step liquid?Cliquid extraction with ethyl acetate-dichloromethane, SYUIQ-F5 and SUCL (internal standard) were subjected to LC?CMS?CMS analysis using positive electro-spray ionization under selected reaction monitoring mode. Chromatographic separation of SYUIQ-F5 and SUCL was achieved on a Zorbax Eclipse Plus C18 column (I.D. 4.6 mm × 150 mm, 3.5 ??m) with a mobile phase consisting of acetonitrile-2 mM ammonium formate (90:10, v/v) at a flow rate of 0.6 mL min^?1. The intra- and inter-batch precision of the method were <12.2 and 8.7%, respectively. The intra- and inter-batch accuracies ranged from 100.2 to 107.3%. The lowest limit of quantification for SYUIQ-F5 was 0.5 ng mL^?1. The method was applied to a SYUIQ-F5 tissue distribution study after an oral dose of 30 mg kg^?1 to rats. SYUIQ-F5 tissue concentrations decreased in the order of small intestine> liver> lung> spleen> stomach> kidneys> heart> brain> muscle> fat> testes> plasma. SYUIQ-F5 could still be detected in most of the tissues at 48-h post-dosing. These results indicated that the LC?CMS?CMS method was sensitive, reliable, and specific to quantify SYUIQ-F5 in different rat tissues.     

Determination of Imidol in Rat Plasma by UPLC�CMS�CMS and Its Application in a Pharmacokinetic Study

A rapid, sensitive and accurate ultra-performance liquid chromatography/tandem mass spectrometry method was developed and validated for the quantitative determination of imidol in rat plasma for the first time. The analyte and internal standard were extracted from plasma by liquid?Cliquid extraction with diethyl ether. The separation was performed on a BEH C₁₈ column (50 mm × 2.1 mm, 1.7 ??m). The detection was carried out by electrospray ionization mass spectrometry in positive ion mode with multiple reaction monitoring. Linear calibration curves were obtained in the concentration range of 2.5?C2,500 ng mL^?1, with the lower limit of quantification of 2.5 ng mL^?1. The intra- and inter-day precision (RSD) values were below 8% and accuracy (RE) was from ?7.9 to 6.3%. After strict validation, the method was applied successfully to the pharmacokinetic study of imidol in rats after oral and intravenous administration, respectively.     

Purification and Characterization of a New Serine Protease with Fibrinolytic Activity from the Marine Invertebrate, Urechis unicinctus

A non-hemorrhagic, chymotrypsin-like serine protease, UFEII, was purified from the marine echiuroid worm, Urechis unicinctus, after a combination of chromatography steps. UFEII was monomeric, with an apparent molecular weight of 26.7 kDa via SDS-PAGE. The isoelectric point of UFEII was 4.03, and the maximum activity of the enzyme was observed at 50 °C and pH?8.0. According to fibrin plate assays, UFEII could not only directly degrade fibrin and fibrinogen but also activate plasminogen. Further, UFEII preferentially hydrolyzed the fibrinogen γ-chain, followed by the Bβ-chains and Aα-chains. Moreover, ufeII, full length of the gene encoding UFEII, was obtained by RT-PCR, degenerated PCR, and nested PCR. The ufeII was determined to be a 906-bp cDNA containing an open reading frame of 795 bp encoding a putative protein of 264 amino acids with a predicted molecular weight of 27.03 kDa. Besides, UFEII exhibited no hemorrhagic effect. Overall, U. unicinctus may represent a potential source of new therapeutic agents in thrombolytic therapy.     

Borated Titania,a New Option for the Selective Enrichment of cis‐Diol Biomolecules

As low abundance cis‐diol biomolecules are of great significance in biological organisms, preparation of materials for the selective enrichment of such compounds is highly favorable for the development of the related proteomics and metabolomics. To this end, we have prepared monolithic borated titania by a non‐aqueous sol‐gel strategy as a new inorganic affinity material for the specific capture of nucleosides, glycopeptides and glycoproteins. Benefiting from the inorganic framework, this material prevented the hydrophobic interference, which was somewhat inevitable for the mainstream organic‐based boronate affinity materials. The prepared material was carefully characterized by scanning electron microscope (SEM), X‐ray diffraction (XRD), X‐ray photoelectron spectroscopy (XPS) and nitrogen‐sorption experiments to investigate the morphology and elemental composition. The excellent performance of borated titania on enrichment of cis‐diol biomolecules was demonstrated by extracting the glycopeptides from horseradish peroxidase (HRP) digestion, standard glycoproteins, and nucleosides from a human‐urine matrix. This kind of inorganic affinity material offers a new option for selective enrichment or separation of cis‐diol biomolecules.