全文获取类型
收费全文 | 73242篇 |
免费 | 15423篇 |
国内免费 | 6789篇 |
专业分类
化学 | 67615篇 |
晶体学 | 856篇 |
力学 | 2614篇 |
综合类 | 420篇 |
数学 | 6517篇 |
物理学 | 17432篇 |
出版年
2024年 | 129篇 |
2023年 | 725篇 |
2022年 | 1328篇 |
2021年 | 1516篇 |
2020年 | 2547篇 |
2019年 | 3794篇 |
2018年 | 2199篇 |
2017年 | 1807篇 |
2016年 | 4965篇 |
2015年 | 5169篇 |
2014年 | 5513篇 |
2013年 | 6706篇 |
2012年 | 6283篇 |
2011年 | 5498篇 |
2010年 | 5174篇 |
2009年 | 5019篇 |
2008年 | 4910篇 |
2007年 | 4112篇 |
2006年 | 3658篇 |
2005年 | 3501篇 |
2004年 | 2988篇 |
2003年 | 2662篇 |
2002年 | 3425篇 |
2001年 | 2510篇 |
2000年 | 2221篇 |
1999年 | 1362篇 |
1998年 | 766篇 |
1997年 | 719篇 |
1996年 | 648篇 |
1995年 | 592篇 |
1994年 | 499篇 |
1993年 | 424篇 |
1992年 | 368篇 |
1991年 | 302篇 |
1990年 | 322篇 |
1989年 | 235篇 |
1988年 | 153篇 |
1987年 | 127篇 |
1986年 | 126篇 |
1985年 | 105篇 |
1984年 | 69篇 |
1983年 | 61篇 |
1982年 | 50篇 |
1981年 | 43篇 |
1980年 | 22篇 |
1979年 | 9篇 |
1978年 | 13篇 |
1977年 | 12篇 |
1976年 | 10篇 |
1973年 | 10篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
961.
A piezoelectric nucleic acid sensor was constructed ofr detection of tumor necrosis factor gene.Two methods were employed for immobilization of nucleic acid probe on gold electrode of piezoelectric crystal.The results show that polyethyleneimine adbesion and glutaraldehyde cross-linking method has higher sensitivity,stability and selectivity than protein A method.The solid-phase nucleic acid hybridization of oligo unclecotides and tumor necrosis factor target gene sequence were monitorde using this sensor.Tumor necrosis factor gene sequence(580bp) was detected by this nucleic acid sensor for the first time. 相似文献
962.
高分子催化剂由于同时具有多相催化剂和均相催化剂的优点,已引起了广泛的重视.然而表面活性组份贵金属的流失直接影响了此类高活性催化剂的推广应用.作者合成了不同载体的几类负载钯催化剂,探讨其在1,5,9-环十二碳三烯的选择加氢反应过程中钯的流失规律.从改变高分子配位体入手,通过调整聚合物载体的物理性能、配位原 相似文献
963.
964.
965.
On-line photochemical reaction of reserpine in the presence of acetone was investigated. Acetone was found to speed up the on-line photochemical conversion of reserpine into an intensively fluorescent compound. Not only reaction acidity but also the acetate buffer concentration affected the on-line photochemical induced fluorescence signal. Based on the observation an automated flow injection photochemical fluorimetric approach was developed. An injected sample zone was carried by a water stream to be merged with a acetate buffer (pH 3.4) solution containing 0.02% acetone in a knotted PTFE reactor (KR), which was freely coiled around a 6-W low pressure mercury lamp. While passing the KR, reserpine was transformed into an intensively fluorescent compound. It was on-line detected in a flow-through cell at the emission wavelength of 490 nm and excitation wavelength of 386 nm. At optimized conditions, a detection limit 0.45 mug l(-1) was achieved at a sampling rate of 90 h(-1). Eleven determinations of a 0.5 mg l(-1) reserpine standard solution gave a R.S.D. of 0.3%. The linear dynamic range of reserpine calibration curve was 0.01-0.75 mg l(-1). The proposed method was applied to assay the reserpine content in tablets and to monitor the dissolution profile of reserpine tablets. Satisfactory results were obtained for both the assays and dissolution studies. 相似文献
966.
A competitive enzyme-linked immunosorbent assay (ELISA) was developed for the quantitative detection of the insect growth regulator fenoxycarb. Polyclonal rabbit antisera, raised against protein conjugates of four haptenic derivatives of fenoxycarb, were utilized in immobilized antigen-based, competitive immunoassays. With ELISA systems that were both hapten- and carrier-heterologous, most antiserum titers fell in the range of 1:1000-1:30,000. Assay conditions, including concentrations of antisera and coating antigens, were optimized. The effect of pH, organic solvents, and various blocking agents was also investigated. A hapten-homologous and two hapten-heterologous indirect ELISAs allowed fenoxycarb determination in the range of 0.1-85 ng ml−1 with apparent IC50 values of 1.2-2.8 ng ml−1. Cross-reactivities with a number of compounds (e.g. pesticides of related structure, hapten synthesis intermediates, fenoxycarb metabolite, photodegradation products) were determined, and the assay proved highly selective for fenoxycarb. In particular, no significant interference was found with selected pyrethroid and juvenile hormone analog insecticides, phenoxyacetic acid herbicides, and photodegradation products of fenoxycarb. Using spiked water samples, assay performance was validated by SPME/GC-MS. 相似文献
967.
J. G. Liu M. H. He H. W. Zhou Z. G. Qian F. S. Wang S. Y. Yang 《Journal of polymer science. Part A, Polymer chemistry》2002,40(1):110-119
Organosoluble polyimides were synthesized with the alicyclic dianhydride 1,8‐dimethylbicyclo[2,2,2]oct‐7‐ene‐2,3,5,6‐tetracarboxylic dianhydride and aromatic diamines. The polyimides possessed good solubility both in strong dipolar solvents and in common solvents; the thermal decomposition temperature of the polyimides exceeded 420 °C. Strong and flexible films of the polyimides, with the cutoff of ultraviolet–visible absorption lower than 310–320 nm, exhibited good features as the alignment layers for nematic liquid crystals with pretilt angles of 1.5–2.9°. © 2001 John Wiley & Sons, Inc. J Polym Sci Part A: Polym Chem 40: 110–119, 2002 相似文献
968.
C. Bibaut‐Renauld D. Burget J. P. Fouassier C. G. Varelas J. Thomatos G. Tsagaropoulos L. O. Ryrfors O. J. Karlsson 《Journal of polymer science. Part A, Polymer chemistry》2002,40(18):3171-3181
The efficiency of several α‐diketones as visible light photoinitiators for the crosslinking of waterborne latex dispersions in the presence of acrylic monomers was evaluated. Among the eight α‐diketones studied, camphorquinone allows the fastest curing speed, and the curing of the acrylic waterborne coating is not affected by the presence of oxygen. The properties of the sunlight‐cured volatile organic compound (VOC)‐free pigmented paints prepared from the waterborne latex are as good or better than the equivalent conventional paint containing VOCs. © 2002 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 40: 3171–3181, 2002 相似文献
969.
970.
开展正常人体内元素含量的检测研究具有重要生物学意义,不仅可充实我国正常成年人脏器组织元素含量值的基础医学数据,为研究人体内微量元素与健康的关系提供科学依据、也为辐射防护领域内放射性核素的剂量估算提供重要参数。本文从以下五个方面进行了论述:()体内元素含量检测研究的目的、意义及其概况;(2)元素含量检测的重要性及其历史沿革;(3)元素的检测方法及质量控制;(4)人体内脏器组织元素含量的数值;(5)与国外资料相比较。本文着重介绍了我国100例正常成年人20个脏器组织(包括胃、小肠、大肠、心、肝、脾、肺、肾、… 相似文献