Measurement of penbutolol and 4-hydroxypenbutolol in plasma or serum by HPLC

A simple HPLC method for penbutolol and 4-hydroxypenbutolol assay has been developed. Plasma or serum (200 microliters) is vortex-mixed (30 s) with Tris solution (2 M, pH 10.6) containing an internal standard (50 microliters) and methyl t-butyl ether (200 microliters). After centrifugation, the extract (100 microliters) is analysed using an unmodified silica column (250 x 5 mm ID) and iso-octane-methanol-methyl t-butyl ether (55:25:20) containing ammonium perchlorate (10 mM, pH 5.7) as eluent and with fluorescence detection. No interference has been encountered and the limit of accurate measurement for both compounds is 5 micrograms/l.     

Diethyl 4‐(2,5‐di­methoxy­phenyl)‐2,6‐di­methyl‐1,4‐di­hydro­pyridine‐3,5‐di­carboxyl­ate,conformational change with solvent of crystallization

The title compound, C₂₁H₂₇NO₆, has been crystallized from ethanol containing nitro­benzene and shows the phenyl ring, B, in an ap conformation. This structure may be compared with that of the mol­ecule crystallized from ethanol alone, in which the B ring is seen in an sp conformation. The isolation of this rotamer has implications for the understanding of the docking of calcium beta‐blocking di­hydro­pyridine mol­ecules with their receptor site.     

Many Polytopes Meeting the Conjectured Hirsch Bound

The still open Hirsch conjecture asserts that Δ(d,n) ≤n-d for all n > d ≥ 2 , where Δ (d,n) denotes the maximum edge-diameter of (convex) d -polytopes with n facets. This paper adds to the list of pairs (d,n) that are known to be H -sharp in the sense that Δ (d,n) ≥ n-d . In particular, it is proved that Δ(d,n)≥ n-d for all n > d ≥ 14 . Received November 20, 1996, and in revised form March 21, 1997.     

Potentiometrie

Ohne Zusammenfassung