Quantitative Dünnschicht-Chromatographie

To avoid errors caused by irregularities of chromatoplates in quantitative TLC often double beam densitometers are used. This paper describes the simulation of different double beam instruments using a single beam scanner and digital computers, to obtain distinct data on the increase in accuracy. The relative standard deviations from a greater number of experiments are: single beam scanner 3–5%, double beam instrument 2–4% and dual wave-length scanner 2.5–4%.