Zur Bestimmung des Mangans in Eisen und Stahl

Ohne Zusammenfassung     

The Structure of an Archaeal B‐Family DNA Polymerase in Complex with a Chemically Modified Nucleotide

Archaeal B‐family DNA polymerases (DNA pols) are the driving force of cutting‐edge biotechnological applications like next‐generation sequencing. The acceptance of chemically modified nucleotides by DNA pols is key to these technologies. Until now, no structural data have been available for these DNA pols in complex with modified substrates, which could build the basis for understanding interactions between the enzyme and the chemically modified nucleotide and for the further development of next‐generation nucleotides. For the first time, we crystallized an exonuclease‐deficient variant of the wild‐type B‐family KOD DNA pol with a modified nucleotide in a closed, ternary complex. We also crystalized the A‐family DNA pol KlenTaq with the same nucleotide. The reported structural data reveal how the protein and the DNA modulate two distinct conformations of the appended moiety in the A‐ and B‐family DNA pols and how these influence the processing of the modified nucleotide. Overall, this study provides first insight into the interplay between B‐family DNA pols and relevant modified substrates.     

A graphical exploration of the Deepwater Horizon oil spill

This paper investigates some of the immediate impacts of the Deepwater Horizon oil spill of 2010 on the environment using graphical means. The exploration focuses on the effects of the oil discharge on wildlife, the chemical pollution in the area following the spill, and salinity levels in the aftermath of the spill. Thousands of animals including birds, turtles, dolphins, and whales were found dead along the beaches and in the Gulf of Mexico in the months after the oil discharge. Levels of polycyclic aromatic hydrocarbons were found to be at dangerous levels along the coast line, making conditions for wildlife highly unfavorable. Salinity measurements, which can be used to determine currents and oil movement, are examined over time as well as geographically.     

Über Rosmarin und Rosmarinsäure

Rosmarinic acid, a tan from Lamiaceae, is found in rosemary and many other plants such as thyme, painted nettle, savory, sage, oregano, or lemon balm, from which it has been isolated here. It is a caffeic acid ester and a chiral, aromatic natural product. Rosmarinic acid tastes bitter, which may be attractive for rosemary as spice. Due to the catechol subunit the acid is an antioxidant. Using rosmarinic acid as a cure is possible due to manyfold physiological effects which are still under investigation. The rosmarinic acid biosynthesis pathway was completely elucidated at the painted nettle. This led to attempts to produce the acid biotechnologically by plant cell cultures in high yields. The preparation of a spectroscopically pure sample is challenging. All analytical spectra were recorded and are reproduced and interpreted either in the main part or in the supporting information.