Quantum circuit theory

The electronic Schroedinger Equation for devices which are small compared to the inelastic scattering length of electrons is solved by a transformation to an equivalent quantum circuit which is numerically tractable. The method is applied to dirty wires and reveals the existence of anomalously conductive states.     

Non-minimal and non-universal supersymmetry

I motivate and discuss non-minimal and non-universal models of supersymmetry and supergravity consistent with string unification at 10¹⁰ GeV.     

Some predictions of a theory of post-ionization of field-evaporated ions

Predictions of post-ionization theory of field evaporation which distinguish it from other theories of field evaporation are made. The expected variation of ion charge state with field strength and the expected energy deficit structure of the ions are presented. Recent experimental evidence for post-ionization is outlined.     

Surface densities of states in the tight-binding approximation

We present a summary of the main results obtained by a new method of calculating the electronic structure of solids and their surfaces in the tight-binding approximation. In addition to being able to reproduce the results of Kalkstein and Soven, we obtain the local density of states at atoms in the low index surfaces of fcc, hcp and bcc d-bands. We also include examples of the effect of surface dilation, the density of states at atoms in subsurface layers, and a very simple example of the adatom density of states.     

Engineered biosynthesis of hybrid macrolide polyketides containing D-angolosamine and D-mycaminose moieties

The glycosylation of natural product scaffolds with highly modified deoxysugars is often essential for their biological activity, being responsible for specific contacts to molecular targets and significantly affecting their pharmacokinetic properties. In order to provide tools for the targeted alteration of natural product glycosylation patterns, significant strides have been made to understand the biosynthesis of activated deoxysugars and their transfer. We report here efforts towards the production of plasmid-borne biosynthetic gene cassettes capable of producing TDP-activated forms of D-mycaminose, D-angolosamine and D-desosamine. We additionally describe the transfer of these deoxysugars to macrolide aglycones using the glycosyl transferases EryCIII, TylMII and AngMII, which display usefully broad substrate tolerance.     

The gene cluster for fluorometabolite biosynthesis in Streptomyces cattleya: a thioesterase confers resistance to fluoroacetyl-coenzyme A

A genomic library of Streptomyces cattleya was screened to isolate a gene cluster encoding enzymes responsible for the production of fluorine-containing metabolites. In addition to the previously described fluorinase FlA which catalyzes the formation of 5'-fluoro-5'-deoxyadenosine from S-adenosylmethionine and fluoride, 11 other putative open reading frames have been identified. Three of the proteins encoded by these genes have been characterized. FlB was determined to be the second enzyme in the pathway, catalyzing the phosphorolytic cleavage of 5'-fluoro-5'-deoxyadenosine to produce 5-fluoro-5-deoxy-D-ribose-1-phosphate. The enzyme FlI was found to be an S-adenosylhomocysteine hydrolase, which may act to relieve S-adenosylhomocysteine inhibition of the fluorinase. Finally, flK encodes a thioesterase which catalyzes the selective breakdown of fluoroacetyl-CoA but not acetyl-CoA, suggesting that it provides the producing strain with a mechanism for resistance to fluoroacetate.     

Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase

The gene cluster encoding biosynthesis of the clinically important glycopeptide antibiotic teicoplanin has been cloned from Actinoplanes teichomyceticus. Forty-nine putative open reading frames (ORFs) were identified within an 89 kbp genetic locus and assigned roles in teicoplanin biosynthesis, export, resistance, and regulation. Two ORFs, designated orfs 1 and 10*, showed significant homology to known glycosyltransferases. When heterologously expressed in Escherichia coli, these glycosyltransferases were shown to catalyze the transfer of UDP-(N-acetyl)-glucosamine onto, respectively, 3-chloro-beta-hydroxytyrosine-6 (3-Cl-6betaHty) and 4-hydroxyphenylglycine-4 (4Hpg) of the teicoplanin heptapeptide aglycone. The product of another ORF, orf11*, was demonstrated in vitro to transfer n-acetyl-, n-butyryl-, and n-octanoyl-groups from acyl-CoA donors either to a free UDP-aminosugar or to an aminosugar moiety in the teicoplanin pseudoaglycone, thus identifying Orf11* as the key acyltransferase in teicoplanin maturation. These findings should accelerate the combinatorial engineering of new and improved glycopeptide drugs.     

Acoustic enhancement of diffusion in a porous material

Lattice Boltzmann simulations are used to model the enhancement of diffusion which results from Eckart (attenuation driven) acoustic streaming in model porous material. Comparisons are made to Fickian diffusion where no flow is present and the diffusion when a fluid jet is used, which represents a more conventional method of enhancement. We show that streaming can produce a higher diffusion rate for the same average flow velocity and propose that this is the result of the continuation within the material of the driving force that produces the acoustic streaming.