Effects of Grafting Density and Film Thickness on the Adhesion of Staphylococcus epidermidis to Poly(2‐hydroxy ethyl methacrylate) and Poly(poly(ethylene glycol)methacrylate) Brushes

Thin polymer films that prevent the adhesion of bacteria are of interest as coatings for the development of infection‐resistant biomaterials. This study investigates the influence of grafting density and film thickness on the adhesion of Staphylococcus epidermidis to poly(poly(ethylene glycol)methacrylate) (PPEGMA) and poly(2‐hydroxyethyl methacrylate) (PHEMA) brushes prepared via surface‐initiated atom transfer radical polymerization (SI‐ATRP). These brushes are compared with poly(ethylene glycol) (PEG) brushes, which are obtained by grafting PEG onto an epoxide‐modified substrate. Except for very low grafting densities (ρ = 1%), crystal violet staining experiments show that the PHEMA and PPEGMA brushes are equally effective as the PEG‐modified surfaces in preventing S. epidermis adhesion and do not reveal any significant variations as a function of film thickness or grafting density. These results indicate that brushes generated by SI‐ATRP are an attractive alternative to grafted‐onto PEG films for the preparation of surface coatings that resist bacterial adhesion.

     

A generalized integro-differential theory of nonlocal elasticity of n-Helmholtz type: part I—analytical formulation and thermodynamic framework

Meccanica - A generalized theory of nonlocal elasticity is elaborated. The proposed integral type nonlocal formulation is based on attenuation functions being assumed as the convolution product of...     

Silica monolithic columns: synthesis, characterisation and applications to the analysis of biological molecules

In recent years, proteomics has been a subject of intense research. The complexity of proteomics samples has fostered technological developments. One of these addresses the need for more efficient and faster separations. Monolithic columns prepared from organic and silica monomers offer very efficient separations at low back-pressure. Silica-based monoliths have small-sized skeletons and a bimodal pore size distribution with microm-sized throughpores and nm-sized mesopores. This gives silica-based monoliths favourable properties for high-efficiency, fast separations, like a low-pressure drop across the column, fast mass transfer kinetics and a high binding capacity.     

Grain boundary corrosion of the surface of annealed thin layers of gold by OH· radicals

Annealed thin layers of gold with large mono-crystalline areas were treated with OH^· radicals generated in an electrochemical Fenton reaction. The morphological changes observed with ex situ atomic force microscopy in non-contact mode and grazing incidence X-ray diffractometry show that the grain boundaries, and generally the non-{111} planes, are the loci of highest reactivity, i.e., the places where the gold dissolution is much faster than on the {111} planes.     

New flag-transitive geometries for the groups Sp_4(K) and SU_5(K)

In Pralle and Shpectorov (Adv Geom 7(1):1–17, 2007) the class of ovoidal hyperplanes in dual polar spaces of rank 4 is described. In this paper we observe that by removing such a hyperplane and a related second hyperplane one obtains a nice geometry for the group stabilising the ovoidal hyperplane. We show that this group acts flag-transitively and that the geometry is simply connected.     

Belting and pop, nonclassical approaches to the female middle voice: Some preliminary considerations

There is a commonly perceived difference in the sound produced in the approximate range D₄-D₅ by female singers in the western opera and concert tradition, on the one hand, and certain other styles, including rock, pop, folk, and some Broadway musicals, on the other. The term “belting” is sometimes used to refer to at least one approach to such “nonclassical” singing. In this study, based on spectrographic, electroglottographic, and sub- and supraglottal pressure measurements on representative voices of the “operatic” and “nonclassical” tradition, acoustic and laryngeal differences between the two traditions are described, and an objective, specific definition of “belting” is offered.     

Mass Spectrometric Imaging of Red Fluorescent Protein in Breast Tumor Xenografts

Mass spectrometric imaging (MSI) in combination with electrospray mass spectrometry (ESI-MS) is a powerful technique for visualization and identification of a variety of different biomolecules directly from thin tissue sections. As commonly used tools for molecular reporting, fluorescent proteins are molecular reporter tools that have enabled the elucidation of a multitude of biological pathways and processes. To combine these two approaches, we have performed targeted MS analysis and MALDI-MSI visualization of a tandem dimer (td)Tomato red fluorescent protein, which was expressed exclusively in the hypoxic regions of a breast tumor xenograft model. For the first time, a fluorescent protein has been visualized by both optical microscopy and MALDI-MSI. Visualization of tdTomato by MALDI-MSI directly from breast tumor tissue sections will allow us to simultaneously detect and subsequently identify novel molecules present in hypoxic regions of the tumor. MS and MALDI-MSI of fluorescent proteins, as exemplified in our study, is useful for studies in which the advantages of MS and MSI will benefit from the combination with molecular approaches that use fluorescent proteins as reporters.