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1.
Jebanathirajah J Steen H Roepstorff P 《Journal of the American Society for Mass Spectrometry》2003,14(7):777-784
Glycosylation is the most widespread protein modification and is known to modulate signal transduction and several biologically important interactions. In order to understand and evaluate the biological role of glycosylation it is important to identify the glycosylated protein and localize the site glycosylation under particular biological conditions. To identify glycosylated peptides from simple mixtures, i.e., in-gel digests from single SDS PAGE bands we performed high resolution, high accuracy precursor ion scanning using a quadrupole TOF instrument equipped with the Q(2) pulsing function. The high resolving power of the quadrupole TOF instrument results in the selective detection of glycan specific fragment ions minimizing the interference of peptide derived fragment ions with the same nominal mass. Precursor ion scanning has been previously described for these glycan derived ions. However the use of this method has been limited by the low specificity of the method. The analysis using precursor ion scanning can be applied to any peptide mixture from a protein digest without having previous knowledge of the glycosylation of the protein. In addition to the low femtomole (nanomolar) detection limits, this method has the advantage that no prior derivatization or enzymatic treatment of the peptide mixtures is required. 相似文献
2.
Keith M Kendrick Yang Zhan Hanno Fischer Alister U Nicol Xuejuan Zhang Jianfeng Feng 《BMC neuroscience》2011,12(1):1-23
Background
How oscillatory brain rhythms alone, or in combination, influence cortical information processing to support learning has yet to be fully established. Local field potential and multi-unit neuronal activity recordings were made from 64-electrode arrays in the inferotemporal cortex of conscious sheep during and after visual discrimination learning of face or object pairs. A neural network model has been developed to simulate and aid functional interpretation of learning-evoked changes.Results
Following learning the amplitude of theta (4-8 Hz), but not gamma (30-70 Hz) oscillations was increased, as was the ratio of theta to gamma. Over 75% of electrodes showed significant coupling between theta phase and gamma amplitude (theta-nested gamma). The strength of this coupling was also increased following learning and this was not simply a consequence of increased theta amplitude. Actual discrimination performance was significantly correlated with theta and theta-gamma coupling changes. Neuronal activity was phase-locked with theta but learning had no effect on firing rates or the magnitude or latencies of visual evoked potentials during stimuli. The neural network model developed showed that a combination of fast and slow inhibitory interneurons could generate theta-nested gamma. By increasing N-methyl-D-aspartate receptor sensitivity in the model similar changes were produced as in inferotemporal cortex after learning. The model showed that these changes could potentiate the firing of downstream neurons by a temporal desynchronization of excitatory neuron output without increasing the firing frequencies of the latter. This desynchronization effect was confirmed in IT neuronal activity following learning and its magnitude was correlated with discrimination performance.Conclusions
Face discrimination learning produces significant increases in both theta amplitude and the strength of theta-gamma coupling in the inferotemporal cortex which are correlated with behavioral performance. A network model which can reproduce these changes suggests that a key function of such learning-evoked alterations in theta and theta-nested gamma activity may be increased temporal desynchronization in neuronal firing leading to optimal timing of inputs to downstream neural networks potentiating their responses. In this way learning can produce potentiation in neural networks simply through altering the temporal pattern of their inputs. 相似文献3.
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Hanno Rund 《Annali di Matematica Pura ed Applicata》1972,93(1):99-110
Summary A special class of hypersurfaces of a Riemannian space is examined, this class being defined by the stipulation that the coefficients
of the third fundamental form be expressible as linear combinations of the coefficients of the first and second fundamental
forms. It is jound that these so-called C-hypersurfaces are umbilical provided that certain conditions (which may depend on
dimension) are satisfied. An (n-1)-dimensional Einstein space imbedded in an n-dimensional space of constant curvature is
such a C-hypersurface; accordingly the theory may be applied to the problem of the local imbedding of such spaces.
Entrata in Redazione il 23 giugno 1971. 相似文献
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Hanno Essn 《International journal of quantum chemistry》1982,21(4):717-726
How the monotonic trend on which the periodicity of the periodic system is superposed is well described by Thomas–Fermi theory is explained. The mathematical structure of the periodicity is elucidated and explained. Algebraic formulas for the key atomic numbers are derived, e.g., the atomic number at which l electrons first appear is given by Zf(??) = 4 (∑ n2)+1. 相似文献
9.
In the setting of vector-valued quantum fields obeying a linear wave-equation in a globally hyperbolic, stationary spacetime,
it is shown that the two-point functions of passive quantum states (mixtures of ground- or KMS-states) fulfill the microlocal
spectrum condition (which in the case of the canonically quantized scalar field is equivalent to saying that the two-pnt function
is of Hadamard form). The fields can be of bosonic or fermionic character. We also give an abstract version of this result
by showing that passive states of a topological *-dynamical system have an asymptotic pair correlation spectrum of a specific
type.
Received: 9 February 2000 / Accepted: 7 June 2000 相似文献
10.
In this paper we investigate the behaviour of the solutions of equations ΣI=1n aixi = b, where Σi=1n, ai = 0 and b ≠ 0, with respect to colorings of the set N of positive integers. It turns out that for any b ≠ 0 there exists an 8-coloring of N, admitting no monochromatic solution of x3 − x2 = x2 − x1 + b. For this equation, for b odd and 2-colorings, only an odd-even coloring prevents a monochromatic solution. For b even and 2-colorings, always monochromatic solutions can be found, and bounds for the corresponding Rado numbers are given. If one imposes the ordering x1 < x2 < x3, then there exists already a 4-coloring of N, which prevents a monochromatic solution of x3 − x2 = x2 − x1 + b, where b ε N. 相似文献