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101.
Evan R. Trivedi Benjamin J. Vesper Hana Weitman Benjamin Ehrenberg Anthony G.M. Barrett James A. Radosevich Brian M. Hoffman 《Photochemistry and photobiology》2010,86(2):410-417
We report the preparation of chiral oxygen atom-appended porphyrazines (pzs) as biomedical optical agents that absorb and emit in the near-IR wavelength range. These pzs take the form M[pz(A4-nBn)], where “A” and “B” represent moieties appended to the pz’s pyrrole entities, A = (2R,3R) 2,3-dimethyl-2,3-dimethoxy-1,4-diox-2-ene, B = β,β′-di-isopropoxybenzo, M is the incorporated metal ion (M = H2, Zn), and n = 0, 1, 2 (-cis/-trans) and 3 ( Scheme 1 ). When dissolved in polar media, H2[pz(trans-A2B2)] 5a does not fluoresce and has a negligible quantum yield for singlet oxygen generation (ФΔ = 0.074 ± 0.001, methanol), as measured by the photo-oxidation of DMA. However, when sequestered in the nonpolar environment of a liposome, it displays strong NIR emission (λ max = 705 nm, Ф f = 0.087) and an extremely high singlet oxygen quantum yield (ФΔ→1). Of this series, H2[pz(trans-A2B2)] 5a is attractive as a potential optical probe, showing strongly fluorescent uptake by cells in culture, while 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide measurements of cell viability show no evidence of dark toxicity. This agent does show significant photoinduced toxicity suggesting that pzs such as 5a have promise as “theranostic” optical agents that can be visualized with fluorescence imaging while acting as a sensitizer for photodynamic therapy.
102.
103.
Hana Dejmkova Matteo Scampicchio Jiri Zima Jiri Barek Saverio Mannino 《Electroanalysis》2009,21(9):1014-1018
A robust electrochemical method to measure the total phenol content in food samples is presented. Under optimal condition, BDD electrode showed excellent performance to detect the oxidation of several phenols and does not present the drawback due to electrode fouling. The analytical method used to perform such measurement has been optimized and successfully applied in different food samples. The results obtained were compared with the standard Folin–Ciocalteau method. 相似文献
104.
Fe-catalyzed cross-coupling reactions of 9-substituted or protected 2,6-dichloropurines with 1 equiv of methylmagnesium chloride gave regioselectively 2-chloro-6-methylpurines in good yields. The same reactions with 3 equiv of methylmagnesium chloride or Pd-catalyzed reactions with trimethylaluminum afforded 2,6-dimethylpurines. The 2-chloro-6-methylpurines underwent another coupling with phenylboronic acid to give 6-methyl-2-phenylpurines. All reactions were perfomed for Bn- and THP-protected purine bases as well as for acyl-protected ribosides and 2-deoxyribosides. After deprotection, free purine bases and nucleosides were obtained. 相似文献
105.
Lee SH Yoshida K Matsushita H Clapham B Koch G Zimmermann J Janda KD 《The Journal of organic chemistry》2004,69(25):8829-8835
Primary ureas have been used as substrates in rhodium-catalyzed N-H insertion reactions with an array of diazocarbonyls. The insertion reaction is efficient and gives excellent selectivity and yields. The products from the insertion reaction with diazoketones cyclize readily in the presence of acid to yield the corresponding imidazolones that can be further derivatized by N-alkylation with alkyl, allyl, and benzyl halides. Alternatively, the imidazolones were treated with phosphorus oxybromide to form the corresponding 2-bromoimidazoles that were further functionalized using a Suzuki coupling reaction. 相似文献
106.
Micellar electrokinetic capillary chromatography (MEKC) has been developed as a promising method for the determination of lignans in plant samples. The separation conditions have been optimized with respect to the different parameters including sodium dodecyl sulfate (SDS) and acetonitrile concentration, pH of the background electrolyte, separation voltage, and capillary temperature. The background electrolyte consisting of 40 mM SDS and 35% acetonitrile in 10 mM tetraborate buffer (pH 9.3) was found to be the most suitable electrolyte for this analysis. The applied voltage of 28 kV (positive polarity) and the capillary temperature 25 degrees C gave the best separation of lignans. The interday reproducibility of the peak areas and the migration times was below 2.0%. The results of MEKC analyses were compared with those obtained by capillary electrochromatography (CEC) and reversed-phase high-performance liquid chromatography (RP-HPLC). The possibilities of using this method for the determination of lignans in drug and in serum samples were also tested. 相似文献
107.
Syntheses of Aminolumazines 6-aminolumazien 2 (1-(6-amino-2,4,7-trioxo-1,2,3,4,7,8-hexahydropteridin-8-yl)-1-deoxy-D -ribitol), a degradation product of russupteridine-yellow I ( 1 ) has been prepared from l-deoxy-l-(2,4,7-trioxo-1,2,3,4,7,8-hexahydeopteridin-8-yl)-D -ribitol ( 5 ) via the azo compound 6 and its reduction with Sn/HCOOH. Condensation of 5-amono-6-(D-ribitylamino)uracil ( 8 ) with parabanic acid ( 9 ) has led to ta synthesis of russupteridine-yellow IV ( 4 ); l-deoxy-l-(2,6,8-trioxo-2,4,5,6,7,8-hexahydro-lH-imidazolo[4,5-g]pteridin-4-yl-D -ribitol), albeit in low yield. 相似文献
108.
Henderson MJ Perriman AW Robson-Marsden H White JW 《The journal of physical chemistry. B》2005,109(44):20878-20886
The structure of the interface generated by a spread layer of beta-casein on an aqueous colloidal poly(silicic) acid subphase is described. The results are compared with data for the protein alone spread at the air/water interface and the silicate solution. Films develop at the air-solution interface and a strong pH dependence of the interaction causing this is demonstrated. Reflectometry with X-rays and neutrons was used to probe the interaction as a function of subphase pH and film compression. Film thickness, tau/A, scattering length density, rho/A(-2), water volume fraction, phi(w), and surface coverage, Gamma/mg m(-2), were used to quantify the interfacial structure. Where possible, the X-ray and neutron data sets were co-refined enabling phi(w) to be evaluated without assumption regarding the protein density. At pH 5-7, strong protein-silicate interaction occurred, the interface comprising three regions: a discrete protein upper layer on top of a 15 +/- 2 A layer of silicated material followed by a diffuse layer that extended into the subphase. 相似文献
109.
Kostecka P Havran L Pivonkova H Fojta M 《Bioelectrochemistry (Amsterdam, Netherlands)》2004,63(1-2):245-248
Mercury film electrodes (MFE) have recently been used in nucleic acid electrochemical analysis as alternatives to the classical mercury drop ones. DNA modified with osmium tetroxide, 2,2'-bipyridine (Os,bipy) can be detected with a high sensitivity at mercury electrodes via measurements of a catalytic osmium signal. In this paper we show that mercury film on a glassy carbon electrode can be used in voltammetric analysis of Os,bipy-modified DNA. Application of the MFE as a detection electrode in double-surface electrochemical DNA hybridization assay involving osmium labeling of target DNA is demonstrated. 相似文献
110.
Andrii Mahun Sabina Abbrent Jiri Czernek Jan Rohlicek Hana Mackov Weihua Ning Rafa Konefa Jií Brus Libor Kobera 《Molecules (Basel, Switzerland)》2021,26(19)
Spikelets NMR spectra are very popular as they enable the shortening of experimental time and give the possibility to obtain required NMR parameters for nuclei with ultrawide NMR patterns. Unfortunately, these resulted ssNMR spectra cannot be fitted directly in common software. For this reason, we developed UWNMRSpectralShape (USS) software which transforms spikelets NMR patterns into single continuous lines. Subsequently, these reconstructed spectral envelopes of the (Q)CPMG spikelets patterns can be loaded into common NMR software and automatically fitted, independently of experimental settings. This allows the quadrupole and chemical shift parameters to be accurately determined. Moreover, it makes fitting of spikelets NMR spectra exact, fast and straightforward. 相似文献