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Cara L Ryan David C Baranowski Babykumari P Chitramuthu Suneil Malik Zhi Li Mingju Cao Sandra Minotti Heather D Durham Denis G Kay Christopher A Shaw Hugh PJ Bennett Andrew Bateman 《BMC neuroscience》2009,10(1):1-22
Background
Progranulin is a secreted high molecular weight growth factor bearing seven and one half copies of the cysteine-rich granulin-epithelin motif. While inappropriate over-expression of the progranulin gene has been associated with many cancers, haploinsufficiency leads to atrophy of the frontotemporal lobes and development of a form of dementia (frontotemporal lobar degeneration with ubiquitin positive inclusions, FTLD-U) associated with the formation of ubiquitinated inclusions. Recent reports indicate that progranulin has neurotrophic effects, which, if confirmed would make progranulin the only neuroprotective growth factor that has been associated genetically with a neurological disease in humans. Preliminary studies indicated high progranulin gene expression in spinal cord motor neurons. However, it is uncertain what the role of Progranulin is in normal or diseased motor neuron function. We have investigated progranulin gene expression and subcellular localization in cultured mouse embryonic motor neurons and examined the effect of progranulin over-expression and knockdown in the NSC-34 immortalized motor neuron cell line upon proliferation and survival.Results
In situ hybridisation and immunohistochemical techniques revealed that the progranulin gene is highly expressed by motor neurons within the mouse spinal cord and in primary cultures of dissociated mouse embryonic spinal cord-dorsal root ganglia. Confocal microscopy coupled to immunocytochemistry together with the use of a progranulin-green fluorescent protein fusion construct revealed progranulin to be located within compartments of the secretory pathway including the Golgi apparatus. Stable transfection of the human progranulin gene into the NSC-34 motor neuron cell line stimulates the appearance of dendritic structures and provides sufficient trophic stimulus to survive serum deprivation for long periods (up to two months). This is mediated at least in part through an anti-apoptotic mechanism. Control cells, while expressing basal levels of progranulin do not survive in serum free conditions. Knockdown of progranulin expression using shRNA technology further reduced cell survival.Conclusion
Neurons are among the most long-lived cells in the body and are subject to low levels of toxic challenges throughout life. We have demonstrated that progranulin is abundantly expressed in motor neurons and is cytoprotective over prolonged periods when over-expressed in a neuronal cell line. This work highlights the importance of progranulin as neuroprotective growth factor and may represent a therapeutic target for neurodegenerative diseases including motor neuron disease. 相似文献32.
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Brock IC Ferguson T Vogel H Bebek C Berkelman K Blucher E Byrd J Cassel DG Cheu E Coffman DM Copie T Crawford G DeSalvo R DeWire JW Ehrlich R Galik RS Gittelman B Gray SW Halling AM Hartill DL Heltsley BK Kandaswamy J Kowalewski R Kreinick DL Kubota Y Lewis JD Mistry NB Mueller J Namjoshi R Nandi S Nordberg E Perticone D Peterson D Pisharody M Riley D Silverman A Stone S Worris M Sadoff AJ Avery P Besson D Garren L Yelton J Bowcock T Kinoshita K Pipkin FM Procario M Wilson R Wolinski J Xiao D 《Physical review D: Particles and fields》1991,43(5):1448-1458
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Haas P Hempstead M Jensen T Kagan H Kass R Behrends S Gentile T Guida JM Guida JA Morrow F Poling R Rosenfeld C Thorndike EH Tipton P Green J Namjoshi R Sannes F Stone R Alam MS Katayama N Kim IJ Sun CR Tanikella V Bortoletto D Chen A Garren L Goldberg M Horwitz N Jawahery A Lubrano P Moneti GC Csorna SE Mestayer MD Panvini RS Word GB Yi X Bean A Bobbink GJ Brock I Engler A Ferguson T Kraemer R Rippich C Sutton R Vogel H Bebek C Berkelman K Blucher E Cassel DG Copie T DeSalvo R DeWire JW 《Physical review letters》1986,56(26):2781-2784
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Haas P Hempstead M Jensen T Johnson DR Kagan H Kass R Baringer P McIlwain RL Miller DH Ng CR Shibata EI Behrends S Guida JM Guida JA Morrow F Poling R Thorndike EH Tipton P Alam MS Katayama N Kim IJ Li WC Lou XC Sun CR Tanikella V Bortoletto D Goldberg M Holmes R Horwitz N Jawahery A Lubrano P Moneti GC Sharma V Shipsey IP Thoma P Csorna SE Letson T Mestayer MD Panvini RS Word GB Bean A Bobbink GJ Brock IC Ferguson T Kraemer RW Vogel H Bebek C Berkelman K Blucher E Cassel DG Copie T DeSalvo R 《Physical review letters》1988,60(16):1614-1617
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Bortoletto D Goldberg M Horwitz N Jain V Mestayer MD Moneti GC Sharma V Shipsey IP Skwarnicki T Thulasidas M Csorna SE Letson T Alexander J Artuso M Bebek C Berkelman K Cassel DG Cheu E Coffman DM Crawford G DeWire JW Drell PS Ehrlich R Galik RS Gittelman B Gray SW Halling AM Hartill DL Heltsley BK Kandaswamy J Katayama N Kreinick DL Lewis JD Mistry NB Mueller J Namjoshi R Nandi S Nordberg E O'Grady C Peterson D Pisharody M Riley D Sapper M Silverman A Stone S Worden H Worris M Sadoff AJ 《Physical review letters》1990,64(18):2117-2120
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