Comparative bioanalytical study of3H-deramciclane in dog plasma, using a gas chromatography-nitrogen-selective detection (GC-NPD), a new GC-radiochemical detection (GC-RD) and a liquid scintillation method

Summary A new, highly sensitive and selective gas chromatography method, using radiochemical detection (GC-DR) was developed for the selective determination of³H-labelled deramciclane and its N-desmethyl metabolite in dog plasma. Inter-day accuracy and precision, as well as system suitability of the GC-RD method was investigated during the method validation. The calibration curve was proved to be linear (r=0.9986) in a wide concentration range (13–1000 ngeqv mL⁻¹) The lower limit of quantitation (LLOQ) was 13.7 ngeqv mL⁻¹, and the limit of the detection (LOD) was 1 ngeqv mL⁻¹. Using this new GC-RD method, plasma levels of³H-labelled deramciclane and its metabolite were determined in dogs, after the administration of a single 10 mg kg⁻¹ oral dose. Pharmacokinetic curves and the calculated pharmacokinetic parameters were compared to those obtained using a previously elaborated gas chromatography-nitrogen selective detection method (GC-NPD) and to those obtained by measuring the plasma level of total radioactivity (liquid scintillation counting, LSC). Pharmacokinetic curves and the calculated pharmacokinetic parameters obtained with the two different gas chromatography detection methods (NPD and RD) showed good correlation. Comparison of these results to those acquired by total radioactivity measurement demonstrated that deramciclane was intensively metabolised. Moreover, the biological half-life (t ₁ ^2/β ) of the unknown metabolites proved to be more than a magnitude longer than the half-life of the parent compound or that of N-desmethyl metabolite. Presented at: Balaton Symposium on High-Performance Separation Methods, Siófok, Hungary, September 3–5, 1997.     

Über die Reduktionsformen einiger nichtenolisierenden aromatischer Ketone und Chinone,welche die Vinylpolymerisation zu initiieren vermögen

It is known that the reduction forms of aromatic carbonyl compounds (ACC) initiate anionic polymerization of vinyl monomers. The ability of the reduction forms to transfer an electron to the interacting molecules is defined also by the electron affinity of the partners. To obtain data about the electron affinity the half-wave potentials of polarographic reduction were measured and compared with the values of the lowest antibonding molecular orbital, according to theHückel's method. A linear correlation between the potentials of polarographic reduction and the energy of the lowest antibonding molecular orbit was found. The results prove the correctness of the previously determined electroaffinity defined by electron transfers between the reduction forms and neutral molecules. From the data of quantum chemical calculation and the polarographic reduction ofACC the changes of the free energy, enthalpy and the equilibrum constant for electron transfer reaction were calculated. The initiation ability of the reduction forms ofACC and the elctron affinity discussed.     

Polarometrische bestimmung von zirkonium(IV) mit tartrazin

A new polarimetric procedure has been developed for the rapid determination of zirconium in the presence of tartrazin as reagent This determination is possible in the presence of uranium.     

Untersuchungen über die Serumproteine mit besonderer Rücksicht auf die Blutgruppen

Ohne Zusammenfassung Die mitgeteilten Untersuchungen haben wir mit Unterstützung der Ungarischen wissenschaftlichen „Széchenyi“-Gesellschaft durchgeführt.