Nine ent-kaurene diterpenoids, leukamenin E, glaucocalyxin A, wangzaozi A, epinodisinol, epinodosin, rabdosin B, rabdosinate, lasiokaurin
and oridonin, were separated from Isodon racemosa (Hemsl) Hara and Isodon japonica (Burm.f) Hara var. galaucocalyx (maxin). The protection against DNA damage induced by hydrogen peroxide in human peripheral blood mononuclear cells (PBMCs)
and inhibition of lipid peroxidation in rat liver microsome were studied with comet assay and thiobarbituric acid reactive
substances determination. All nine diterpenoids showed concentration-dependent protection in DNA damage in human PBMCs induced
by 50 μM H2O2. The effects were similar and weaker than quercetin. The inhibitions of lipid peroxidation were obviously difference. Rabdosin
B and oridonin were the strongest inhibitors, followed by lasiokaurin and wangzaozin A. Rabdosinate was the weakest and others
were medium. The structure-activity relationship is discussed. 相似文献
This work was designed to assess the holistic efficacy of concurrent treatment with huperzine A (HA) and ligustrazine phosphate (LP) in the animal model, and to interpret the metabonomics characteristics of the poly-therapy in treating Alzheimer’s disease (AD). Metabolic profiling of the brain was performed using ultra performance liquid chromatography coupled to time-of-flight mass spectroscopy (UPLC-TOF MS). Principal component analysis and partial least squares discriminant analysis were utilized to classify and reveal the differences among the control, model and treatment groups. Ten potential biomarkers have been finally gained. The metabonomic results revealed that the coadministration of HA and LP might slow down the progression of AD through synergistic therapeutic efficacies, exhibiting better effect on amnesia compared with the mono-drug therapy. Importantly, the UPLC-TOF MS-based metabonomic approach would be a useful technique for the diagnosis of AD, the discovery of metabolic biomarkers and the evaluation of the treatment effects.
A beta-cyclodextrin-coated electrode incorporating carbon nanotubes was constructed and applied to the detection of uric acid in the presence of high concentration of ascorbic acid. The major obstacle of the overlapped oxidation potential of ascorbic acid was overcome owing to the distinct ability of the carbon nanotubes-modified electrode to yield a large anodic peak difference ca. 400 mV. The sensitive detection of uric acid has been further improved by the formation of a supramolecular complex between beta-cyclodextrin and uric acid. A linear calibration curve was obtained for 5 x 10(-7) to 5 x 10(-5) M in 0.2 M HAc-NaAc buffer (pH 4.5) with correlation coefficient of 0.998 and detection limit of 0.2 microM. The practical analytical application was illustrated by a selective measurement of uric acid in human urine without any preliminary treatment. 相似文献