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排序方式: 共有493条查询结果,搜索用时 46 毫秒
31.
Uri Yermiyahu Shlomo Nir Gozal Ben-Hayyim Uzi Kafkafi Gunther F. E. Scherer Thomas B. Kinraide 《Colloids and surfaces. B, Biointerfaces》1999,14(1-4):237-249
The hypotheses that genotypic differences in salinity tolerance may result from (i) differences in global surface charge density or (ii) from differences in global Ca2+ binding were tested. An attempt was made to correlate the differing salinity tolerance of four melon cultivars with surface properties of vesicles extracted from the plasma membrane (PM) of their root cells. Surface characterization involved measurements of electrophoretic mobility and sorption of 45Ca2+ to the vesicles in the presence of varying concentrations of Ca2+, Na+ and Mg2+. Irrespective of salinity tolerance, vesicles from the four cultivars yielded similar ζ potentials under similar conditions, indicating similar global surface charge densities. Sorption studies with vesicles from two cultivars differing in salinity tolerance predicted independently this result of equal surface charge density. The estimated global binding affinities of Ca2+, Na+ and Mg2+ to the PM of both cultivars were the same with binding coefficients of 50, 0.8 and 9 M−1, respectively. Consequently, the hypotheses enumerated above to interpret genotypic differences in salinity toxicity are rejected. However, vesicles from the salt-resistant strain sorbed 19% more Ca2+ per given amount of protein in the membrane, indicating the existence of a larger number of negatively charged surface sites per given amount of protein and a smaller amount of protein per given area of membrane. Genotypic differences in site-specific Ca2+-binding affinity (e.g. at ion channels) remain a viable hypothesis for genotypic differences in salinity tolerance. 相似文献
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33.
Binary mixtures of ethylene glycols HO(CH2CH2O)xH (x = 3–5) with glycerol were investigated by fast atom bombardment mass Spectrometry. The relative intensities in the ethylene glycol partial mass spectra as a function of concentration exhibit a steep slope in the low concentration range. With increasing concentration the curves asymptotically approach the 100% line. This effect increases from triethylene to pentaethylene glycol. Comparison of these results with those of surface tension measurements leads to the conclusion that the high sensitivity of fast atom bombardment mass Spectrometry for ethylene glycols is caused by the surface-active properties of these compounds, which lead to an enrichment of the surface investigated with the substance molecules. 相似文献
34.
35.
One-dimensional NMR spectroscopy has proven to be a powerful technique for screening compound libraries in drug discovery. We report a novel water ligand-observed gradient spectroscopy (WaterLOGSY) pulse sequence, named Aroma WaterLOGSY, that selectively detects aromatic WaterLOGSY signals from compounds or ligands. In the Aroma WaterLOGSY, water magnetization is untouched after water excitation and utilizes the whole period of the remaining pulse sequence to relax back to the +z direction. Due to the phase cycling design, the water magnetization is allowed to relax for the period of two full scans before it gets inverted again. Therefore, the recycle delay can be significantly shortened. Within similar experimental time, Aroma WaterLOGSY shows approximately two times higher sensitivity than the standard scheme. This method also allows the use of non-deuterated reagents, thereby accelerating experimental set-up time for ligand-binding studies. 相似文献
36.
Marco L. Hennrich Shabaz Mohammed A. F. Maarten Altelaar Albert J. R. Heck 《Journal of the American Society for Mass Spectrometry》2010,21(12):1957-1965
Here, we explore a de novo sequencing strategy in which we combine Lys-N protein digestion with differential isotopic dimethyl
labeling to facilitate the (de novo) identification of multiply charged peptides in ESI-MS, both under CID and ETD conditions.
For a large fraction of the Lys-N generated peptides, all primary amines are present at the N-terminal lysine, enabling specific
labeling of the N-terminus. Differential derivatization of only the peptide N-terminus in combination with the simultaneous
fragmentation of the corresponding isotopologues allows the straightforward distinction of N-terminal fragments from C-terminal
and internal fragments. Furthermore, also singly and multiply charged N-terminal fragments can easily be distinguished due
to the mass differences of the isotope labeled fragment pairs. As a proof of concept, we applied this approach to proteins
isolated from an avocado fruit, and were able to partially de novo sequence and correctly align, with green plant homologues,
a previously uncharacterized avocado ascorbate peroxidase. 相似文献
37.
Damm M Holzer M Radspieler G Marsche G Kappe CO 《Journal of chromatography. A》2010,1217(50):7826-7832
An efficient microwave-assisted high-throughput protein hydrolysis protocol was developed utilizing strongly microwave absorbing silicon carbide-based microtiter platforms. The plates are equipped with 20 bore holes having the proper dimensions for holding standard screw-capped HPLC/GC vials. Due to the possibility of heating up to four heating platforms simultaneously (80 vials), parallel microwave-assisted acid hydrolyses can be performed under carefully controlled conditions significantly reducing the overall time required for protein hydrolysis and the subsequent evaporation step required for larger volumes of acid. An extensive optimization of the hydrolysis conditions has demonstrated that 5min irradiation at 160°C with 6N HCl leads to comparable results in terms of total and individual amino acid recovery as the traditional method requiring 24h heating at 110°C. Complete hydrolysis of several proteins and synthetic peptides was performed using 25μg of sample material and 100μL of 6N HCl in a dedicated low-volume HPLC/GC vial. Since the hydrolysis and subsequent analysis can be performed from the same vial, errors caused by sample transfer can be minimized. Control experiments have demonstrated that the observed rate enhancements are the result of a purely thermal/kinetic effect as a consequence of the considerable higher reaction temperatures. 相似文献
38.
Tonietto Serena Nestola Fabrizio Redhammer Gunther J. Silvestri Alberta Molin Gianmario Bruno Marco 《Applied Physics A: Materials Science & Processing》2011,103(1):207-212
The mineral inclusions of two orange glass tesserae from paleo-Christian mosaics were investigated in order to derive the
melting temperature reached during their production (sourced from Padua and Vicenza, Veneto region, Italy). In particular,
clinopyroxene crystals were studied by single-crystal X-ray diffraction and electron microprobe WDS analysis. The crystals
show C2/c symmetry, typical of disordered Ca/Na and Mg/Al distributions indicating high-temperature of formation (>700°C). The cation
site populations were obtained by combining results from the two experimental techniques enabled us to derive the following
stoichiometric formula:
lM2[Ca0.819Na0.172Mn0.006K0.003]M1[Mg0.765Fe3+0.210 Cu0.015Ti0.006Zn0.006]T[Si1.933Al0.037Sn0.024]O6\begin{array}{l}{}^{M2}[\mathrm{Ca}_{0.819}\mathrm{Na}_{0.172}\mathrm{Mn}_{0.006}\mathrm{K}_{0.003}]{}^{M1}[\mathrm{Mg}_{0.765}\mathrm{Fe}^{3+}_{0.210}\\[3pt]\quad{}\mathrm{Cu}_{0.015}\mathrm{Ti}_{0.006}\mathrm{Zn}_{0.006}]{}^{T}[\mathrm{Si}_{1.933}\mathrm{Al}_{0.037}\mathrm{Sn}_{0.024}]\mathrm{O}_{6}\end{array} 相似文献
39.
Kumar R Spuesens T Mechet P Kumar P Raz O Olivier N Fedeli JM Roelkens G Baets R Van Thourhout D Morthier G 《Optics letters》2011,36(13):2450-2452
Using a 7.5 μm diameter disk fabricated with III-V-on-silicon fabrication technology, we demonstrate bias-free all-optical wavelength conversion for non-return-to-zero on-off keyed pseudorandom bit sequence (PRBS) data at the speed of 10 Gbits/s with an extinction ratio of more than 12 dB. The working principle of such a wavelength converter is based on free-carrier-induced refractive index modulation in a pump-probe configuration. We believe it to be the first bias-free on-chip demonstration of all-optical wavelength conversion using PRBS data. All-optical gating measurements in the pump-probe configuration with the same device have revealed that it is possible to achieve wavelength conversion beyond 20 Gbits/s. 相似文献
40.
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