Mesoscale chiroptics of rhythmic precipitates

Rhythmic precipitates of centrosymmetric phthalic acid were analyzed by a square-wave mechanically modulated circular extinction imaging microscope. Spherulites were bisected into square-millimeter sized heterochiral domains that are a consequence of circular intensity differential scattering of left and right circularly polarized light. The dissymmetry and chiral amplification indicated optically was confirmed in the microtexture established by atomic force and scanning electron microscopies.     

Ferromagnetic micropallets for magnetic capture of single adherent cells

We present a magnetic micropallet array and demonstrate its capacity to recover specific, individual adherent cells from large populations and deliver them for downstream single cell analysis. A ferromagnetic photopolymer was formulated, characterized, and used to fabricate magnetic micropallets, which are microscale pedestals that provide demarcated cell growth surfaces with preservation of biophysical properties including photopatternability, biocompatibility, and optical clarity. Each micropallet holds a single adherent cell in culture, and hundreds of thousands of micropallets comprise a single micropallet array. Any micropallet in the array can be recovered on demand, carrying the adhered cell with it. We used this platform to recover selectively single cells, which were subsequently analyzed using single-cell RT-qPCR.