Chimeric Antigen Receptor (CAR) T cell immunotherapy is revolutionizing treatment for patients suffering from B-cell lymphoma (BL). However, the current method of CAR T cell production is complicated and expensive, requiring collection of patient blood to enrich the T cell population, ex vivo engineering/activation, and quality assessment before the patient can receive the treatment. Herein we leverage Spleen Selective ORgan Targeted (SORT) Lipid Nanoparticles (LNPs) to produce CAR T cells in situ and bypass the extensive and laborious process currently used. Optimized Spleen SORT LNPs containing 10 % 18 : 1 PA transfected CD3+, CD8+, and CD4+ T cells in wild-type mice. Spleen SORT LNPs delivered Cre recombinase mRNA and CAR encoding mRNA to T cells in reporter mice and in a lymphoreplete B cell lymphoma model (respectively) after intravenous injection without the need for active targeting ligands. Moreover, in situ CAR T cells increased the overall survival of mice with a less aggressive form of B cell lymphoma. In addition, in situ transfected CAR T cells reduced tumor metastasis to the liver by increasing tumor infiltrating lymphocytes. Overall, these results offer a promising alternative method for CAR T cell production with pre-clinical potential to treat hematological malignancies. 相似文献
The anionic solution polymerization of buta‐1,3‐diene was modeled, considering the reactivity of the active sites to be different due to varying geometric configurations. With the first‐order Markov model, expressions for the fraction of active sites and dyad distribution were obtained. The rate constants were determined by fitting to the conversion and dyad experimental data using the nonlinear least squares method. The kinetic model shows that the microstructure and the dyads do not depend on initiator and butadiene concentration but only on rate constants. Without a modifier, the butadiene addition mechanism is entropic; with a modifier, the mechanism changes to entropic‐enthalpic.
The reactions of [Ag(NH=CMe2)2]ClO4 with cis-[PtCl2L2] in a 1:1 molar ratio give cis-[PtCl(NH=CMe2)(PPh3)2]ClO4 (1cis) or cis-[PtCl(NH=CMe2)2(dmso)]ClO4 (2), and in 2:1 molar ratio, they produce [Pt(NH=CMe2)2L2](ClO4)2 [L = PPh3 (3), L2= tbbpy = 4,4'-di-tert-butyl-2,2'-dipyridyl (4)]. Complex 2 reacts with PPh3 (1:2) to give trans-[PtCl(NH=CMe2)(PPh3)2]ClO(4) (1trans). The two-step reaction of cis-[PtCl2(dmso)2], [Au(NH=CMe2)(PPh3)]ClO4, and PPh3 (1:1:1) gives [SP-4-3]-[PtCl(NH=CMe2)(dmso)(PPh3)]ClO4 (5). The reactions of complexes 2 and 4 with PhICl2 give the Pt(IV) derivatives [OC-6-13]-[PtCl3(NH=CMe2)(2)(dmso)]ClO4 (6) and [OC-6-13]-[PtCl2(NH=CMe2)2(dtbbpy)](ClO4)2 (7), respectively. Complexes 1cis and 1trans react with NaH and [AuCl(PPh3)] (1:10:1.2) to give cis- and trans-[PtCl{mu-N(AuPPh3)=CMe2}(PPh3)2]ClO4 (8cis and 8trans), respectively. The crystal structures of 4.0.5Et2O.0.5Me2CO and 6 have been determined; both exhibit pseudosymmetry. 相似文献
Nanocontainers composed of zeolite L crystals were filled with fluorescent dyes and used as the core of a silica “box‐in‐a‐box” construction as in Russian matryoshka dolls, as described by A. Guerrero‐Martínez, L. de Cola, and co‐workers in their Communication on page 1266 ff. The anisotropic cores and isotropic shells of the multicolor emitting systems are separated by a polyelectrolyte layer and can be addressed independently. This approach opens fascinating routes for the construction of multifunctional nanosystems.
Hemibenzopinacolate radicals may be incorporated in a polymer chain and the latter used as a macromolecular initiator. Kinetics of polymerization are strongly dependent on the substitution of the pinacolate. A large family of substituted (CH3)3SiOC(C6H5)2 radicals has been synthesized and studied by electron spin resonance (ESR) and semiempirical molecular orbital calculations. ESR spectra are well resolved and, except for one derivative, allow an unambiguous determination of the hyperfine coupling splitting (HFS) constants. The ground state properties have been calculated within different approximations, namely MNDO, AM1 and PM3 for the geometry optimization and INDO for spin density. Geometries obtained within AM1 and PM3 approximations are satisfactory. The HFS constants evaluated using McConnell type relations and spin densities are in a quite good agreement with the experimental ones. It turns out that the effect of substitution on kinetics of polymerization cannot be attributed to any drastic change in the charge distribution. 相似文献
From electrical resistivity measurements in the range from 240 to 450 K and from optical absorption measurements the energy gap value of CuIn5Se8 has been found to be 1.13 eV. Thermally stimulated current and electrical measurements at high temperature performed in indium annealed samples show deep levels at 0.55 and 0.79 eV, respectively. These defects are expected to be associated with interstitial indium or indium in copper site because of the In‐rich condition. 相似文献