Levels of mineral nutrients in fresh- and frozen bulk hydrated biological specimens: A comparison of EDS data collected in the environmental SEM and a conventional cryo-SEM

Energy-dispersive X-ray microanalysis (EDS) was compared in fresh- and frozen bulk hydrated tissues using the Environmental SEM (ESEM) and conventional cryo-SEM, respectively. Analyses of globoid inclusions of Eucalyptus calophylla seed from two soil types demontsrated that higher levels of cations (K, Ca, Mg, Al, Fe, Mn, Cu, Zn) occurred in seeds from soils containing higher levels of Al, while EDS-detectable levels of S and P were dependent upon the techniques utilised. Cumulative changes in ESEM-EDS-detectable levels of S and P were characterised by collecting cumulative spectra from nutrient standards and compared with those for K. Progressive increases in K occurred and were consistent with an enriching effect. Levels of S and P increased during early analysis (40–60 sec live time) and decreased thereafter. The semi-conductive nature of biological samples, the loss of anions and gain of cations from the net negatively-charged electron interaction volume contributed to an electrochemical bias. These local modifications in fluid chemistry were reversible. Dehydration effects also occurred in stable, “wet‘ samples. These differences indicated that EDS in ESEM may be limited to cations rather than anions, and that changes in fluid electrochemistry and dehydration may affect the level and distribution of elements.     

A comparison of quasi-static uniaxial-strain and hugoniot tests for quartz-phenolic composite

The purpose of this paper is to show that, within experimental uncertainty, the change in volume with stress obtained by quasi-static uniaxial-strain tests matches that obtained by hugoniot experiments over the same pressure range for quartz phenolic. The result of these tests shows that comparing the data by both techniques is meaningful. In addition, the use of the relatively simple and inexpensive quasi-static uniaxial-strain test (strain rates of 10^?4/sec) may provide designers and materials engineers a method for rapid surveying of materials for their hugoniot properties.     

High-strain-rate tests on titanium 6-6-2 utilizing a unique rate-testing machine

This paper presents the design of a unique materials-testing system capable of medium strain rates of from 10^?4 to 10²/s. The design incorporates both closed-loop hydraulic operation with that of open-loop pneumatic operation. A novel design permits accurate specimen alignment and a stiff frame which exceeds 17×10⁶ lb/in. (11.7×10⁴ MPa). The mechanine is able to perform conventional tension/compression tests, fatigue tests and, with slight modification, biaxial-stress-tube tests and triaxial-stress tests. The accurate alignment capability coupled with high frame stiffness and the pneumatic operation enables the testing of brittle materials with rigid grips. Titanium 6-6-2 was tested in both tension and compression at strain rates from 10^?4 to about 10/s at four selected temperatures. The material showed a slight strain-rate sensitivity. Yield stress was shown to increase with strain rate while ductility decreased at each test temperature.     

Application of rainbow schlieren deflectometry for concentration measurements in an axisymmetric helium jet

 The rainbow schlieren deflectometry technique was used to measure oxygen concentrations in a laminar, isothermal helium jet discharged vertically into ambient air. The concentration distributions were inferred from the color schlieren image by taking into consideration the sampling interval and noise in measurements, especially near the jet center. Excellent quantitative agreement was reached between measurements from schlieren and a continuous sampling probe. This work demonstrates the capability of the schlieren technique for providing accurate, spatially-resolved, nonintrusive, full-field of view measurements of species concentration in an isothermal binary system. Because the basic quantity measured is the refractive index, the present schlieren technique can be extended for quantitative measurements of other scalar flow properties related to the refractive index. Received: 21 April 1997 / Accepted: 14 November 1997     

The Cancer Genetics Network: recruitment results and pilot studies

OBJECTIVE: The National Cancer Institute established the Cancer Genetics Network (CGN) to support collaborative investigations into the genetic basis of cancer susceptibility, explore mechanisms to integrate this new knowledge into medical practice, and identify ways of addressing the associated psychosocial, ethical, legal, and public health issues. SUBJECTS AND METHODS: The CGN has developed the complex infrastructure required to support the projects, including the establishment of guidelines and policies, uniform methods, standard questionnaires to be used by all of the centers, and a standard format for submission of data to the Informatics Center. Cancer patients and their family members have been invited to enroll and be included in a pool of potential study participants. The Information Technology Group is responsible for support of the design, implementation, and maintenance of the multicenter Network-wide research protocols. RESULTS: As of January 2004, the CGN contained data on 23,995 probands (participants) and 425,798 family members. As a resource for cancer genetic studies, the CGN has a large number of probands and first-degree relatives with and without cancer and with multiple ethnicities. Different study designs can be used including case-control, case-case, and family studies. CONCLUSIONS: The unique resources of the CGN are available for studies on cancer genetic susceptibility, translational research, and behavioral research. The CGN is now at a point where approved collaborators may have access to enrolled patients and their families for special studies, as well as to the clinical, environmental and family cancer history data banked in the Informatics Center.     

Study of magnetoresistance and conductance of bicrystal grain boundary in La0.67Ba0.33MnO3 thin film

La_0.67Ba_0.33MnO₃ (LBMO) thin film is deposited on a 36.7°C SrTiO₃ bicrystal substrate using laser ablation technique. A microbridge is created across bicrystal grain boundary and its characteristics are compared with a microbridge on the LBMO film having no grain boundary. Presence of grain boundary exhibits substantial magnetoresistance ratio (MRR) in the low field and low temperature region. Bicrystal grain boundary contribution in MRR disappears at temperature T>175 K. At low temperature, I-V characteristic of the microbridge across bicrystal grain boundary is nonlinear. Analysis of temperature dependence of dynamic conductance-voltage characteristics of the bicrystal grain boundary indicates that at low temperatures (T<175 K) carrier transport across the grain boundary in LBMO film is dominated by inelastic tunneling via pairs of manganese atoms and tunneling through disordered oxides. At higher temperatures (T>175 K), magnetic scattering process is dominating. Decrease of bicrystal grain boundary contribution in magnetoresistance with the increase in temperature is due to enhanced spin-flip scattering process.