Development of a multiplex system to assess DNA persistence in taphonomic studies

In this study, we have developed a PCR multiplex that can be used to assess DNA degradation and at the same time monitor for inhibition: primers have been designed to amplify human, pig, and rabbit DNA, allowing pig and rabbit to be used as experimental models for taphonomic research, but also enabling studies on human DNA persistence in forensic evidence. Internal amplified controls have been added to monitor for inhibition, allowing the effects of degradation and inhibition to be differentiated. Sequence data for single‐copy nuclear recombination activation gene (RAG‐1) from human, pig, and rabbit were aligned to identify conserved regions and primers were designed that targeted amplicons of 70, 194, 305, and 384 bp. Robust amplification in all three species was possible using as little as 0.3 ng of template DNA. These have been combined with primers that will amplify a bacterial DNA template within the PCR. The multiplex has been evaluated in a series of experiments to gain more knowledge of DNA persistence in soft tissues, which can be important when assessing what material to collect following events such as mass disasters or conflict, when muscle or bone material can be used to aid with the identification of human remains. The experiments used pigs as a model species. When whole pig bodies were exposed to the environment in Northwest England, DNA in muscle tissue persisted for over 24 days in the summer and over 77 days in the winter, with full profiles generated from these samples. In addition to time, accumulated degree days (ADD) were also used as a measure that combines both time and temperature—24 days was in summer equivalent to 295 ADD whereas 77 days in winter was equivalent to 494 ADD.     

On the nonlinear interpretation of a vibrating wire viscometer operated at large amplitude

A non-linear interpretation is proposed for the vibrating wire (VW) viscometer operated with the transient or ringdown method. This new interpretation is motivated by the necessity of having an acceptable signal to noise ratio (SNR) such that a measurement can be performed in 1 s. A large SNR is achieved by a large oscillation amplitude such that the requisite conditions for the linear interpretation are no longer met. We demonstrate the applicability of a new non-linear interpretation with wires of length 40 mm and 50 mm over a viscosity range from 0.3 to 159 mPa s where the maximum amplitude of motion is approximately two thirds of the radius.     

Validation of a bioanalytical method for the quantification of a therapeutic peptide, ramoplanin, in human dried blood spots using LC-MS/MS

A method has been developed and validated for the quantification of ramoplanin, a 2554 Da peptide antibiotic, in human dried blood spots using high‐performance liquid chromatography with tandem mass spectrometric detection. The validation data meet FDA acceptance criteria for bioanalytical assays and cover the quantification of ramoplanin over the range 10–5000 ng/mL. The assay determines ramoplanin at the same lower limit of quantification as conventional liquid sample methods. Dried blood spot analysis provides an approach for quantification of peptide therapeutics and delivers significant benefits for sample collection and handling and also sample cleanup over conventional plasma and serum assays. Copyright © 2010 John Wiley & Sons, Ltd.     

Do forecasts expressed as prediction intervals improve production planning decisions?

A number of studies have shown that providing point forecasts to decision makers can lead to improved production planning decisions. However, point forecasts do not convey information about the level of uncertainty that is associated with forecasts. In theory, the provision of prediction intervals, in addition to point forecasts, should therefore lead to further enhancements in decision quality. To test whether this is the case in practice, participants in an experiment were asked to decide on the production levels that were needed to meet the following week’s demand for a series of products. Either underproduction cost twice as much per unit as overproduction or vice versa. The participants were supplied with either a point forecast, a 50% prediction interval, or a 95% prediction interval for the following week’s demand. The prediction intervals did not improve the quality of the decisions and also reduced the propensity of the decision makers to respond appropriately to the asymmetry in the loss function. A simple heuristic is suggested to allow people to make more effective use of prediction intervals. It is found that applying this heuristic to 85% prediction intervals would lead to nearly optimal decisions.     

Chemistry: progress since 1860—reflections on chemistry and chemistry education triggered by reading Muspratt’s Chemistry

Foundations of Chemistry - This paper was inspired by the author’s fortunate acquisition of a copy of an original copy of “Muspratt’s Chemistry” that was published in 1860....     

Hydroxy Groups Enhance [2]Rotaxane Anion Binding Selectivity

We report the synthesis of two [2]rotaxanes containing an interlocked three dimensional binding cavity formed from a pyridinium bis(amide) axle component containing two phenol donors, and an isophthalamide based macrocycle. In the competitive solvent mixture 1 : 1 CDCl₃ : CD₃OD, one of the receptors exhibits a much higher selectivity preference for chloride than an analogous rotaxane without the hydroxy groups. X-ray crystal structures reveal the chloride anion guest encapsulated within the interlocked binding cavity, though not all of the hydrogen bond donors are utilised. Computational semi-empirical simulations indicate that secondary intermolecular interactions occur between the axle hydroxy hydrogen bond donors and the [2]rotaxane macrocycle components, contributing to a more preorganised binding pocket, which may be responsible for the observed enhanced selectivity.