A biofunctional polymeric coating for microcantilever molecular recognition

An innovative route to activate silicon microcantilevers (MCs) for label free molecular recognition is presented. The method consists in coating the underivatized MCs with a functional ter-polymer based on N,N-dimethylacrylamide (DMA) bearing N-acryloyloxysuccinimide (NAS) and 3-(trimethoxysilyl)propyl-methacrylate (MAPS), two functional monomers that confer to the polymer the ability to react with nucleophilic species on biomolecules and with glass silanols, respectively. The polymer was deposited onto MCs by dip coating. Polymer coated MCs were tested in both static and dynamic modes of actuation, featuring detection of DNA hybridization as well as protein/protein interaction. In the dynamic experiments, focused on protein detection, the MCs showed an average mass responsivity of 0.4 Hz/pg for the first resonant mode and of 2.5 Hz/pg for the second resonant mode. The results of the static experiments, dedicated to DNA hybridization detection, allowed for direct estimation of the DNA duplex formation energetics, which resulted fully consistent with the nominal expected values. These results, together with easiness and cheapness, high versatility, and excellent stability of the recognition signal, make the presented route a reliable alternative to standard SAM functionalization (for microcantilevers (MCs) and for micro-electro-mechanical systems (MEMS) in general).     

Kolloide Mischlösungen von Kalziumkarbonat und Kalziumphosphat

Ohne Zusammenfassung     

Sulle soluzioni non analitiche dell'equazione funzionalef(x 2)-[f(x)]2=kx e su quelle analitiche dell'equazionef(x α)=λ[f(x)]b

Annali di Matematica Pura ed Applicata (1923 -) -     

Sopra alcune limitazioni per la sollecitazione elastica e sopra la dimostrazione del principio del De Saint Venant

Sunto L'A, studia in questa Memoria la distribuzione delle tensioni interne in un solido elastico convesso quando gli spostamenti, dati in superficie, sono diversi da zero solo in una piccola zona. Nei campi a due dimensioni il risultato è esteso al caso in cui sul contorno siano date le forze; si giunge così ad una dimostrazione del principio del De Saint Venant.