全文获取类型
收费全文 | 122篇 |
免费 | 4篇 |
专业分类
化学 | 96篇 |
力学 | 4篇 |
数学 | 4篇 |
物理学 | 22篇 |
出版年
2023年 | 1篇 |
2021年 | 1篇 |
2020年 | 1篇 |
2019年 | 2篇 |
2017年 | 1篇 |
2016年 | 3篇 |
2015年 | 1篇 |
2014年 | 3篇 |
2013年 | 4篇 |
2012年 | 14篇 |
2011年 | 7篇 |
2010年 | 8篇 |
2009年 | 6篇 |
2008年 | 11篇 |
2007年 | 4篇 |
2006年 | 11篇 |
2005年 | 5篇 |
2004年 | 10篇 |
2003年 | 10篇 |
2002年 | 7篇 |
2000年 | 3篇 |
1998年 | 1篇 |
1996年 | 2篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
排序方式: 共有126条查询结果,搜索用时 0 毫秒
31.
32.
Jan C. van Dam Michael R. Eman Johannes Frank Hans C. Lange Gijs W. K. van Dedem Sef J. Heijnen 《Analytica chimica acta》2002,460(2):307-218
The purpose of this study is to selectively and quantitatively analyze several glycolytic intermediates in cells of Saccharomyces cerevisiae using high-performance anion exchange chromatography (HPAEC) coupled to electrospray ionization tandem mass spectrometry for the analysis. A sodium hydroxide gradient is used to separate the glycolytic compounds and after the column sodium hydroxide is reduced by proton exchange with a membrane device prior to introduction to the mass spectrometer. The detection limits for 10 μl samples are down to the 0.4–5 pmol range. This corresponds for the intracellular metabolites to a range of 2–20 nmol per gram biomass dry weight (DW). Standard addition did reveal some influence of the sample matrix on the measured concentrations. Separation and analysis is hardly affected by the high sulfate and phosphate concentrations (1 mM) in the fermentation medium and by the intracellular matrix. Validation of the glucose-6-phosphosphate LC–MS–MS analysis results with enzymatic analysis showed an excellent agreement between the two methods. The suitability of the method was clearly shown by analyzing a series of steady state S. cerevisiae samples from a carbon limited aerobic chemostat culture. 相似文献
33.
34.
G A De Ruiter P Smid H A Schols J H Van Boom F M Rombouts 《Journal of chromatography. A》1992,584(1):69-75
Fungal carbohydrate antigens were analysed by high-performance immunoaffinity chromatography (HPIAC) with immunoglobulin G (IgG) antibodies raised against extracellular polysaccharides of Mucor racemosus. The protein A-IgG complex was covalently bound with dimethyl pimelimidate, which enabled the use of strong acidic buffers to release the tightly bound antigens from the column. Prior to pulsed-amperometric detection, an anion-micromembrane suppressor was used to raise the pH of the effluent to above 12 without dilution. The HPIAC system provides a sophisticated method for the rapid and sensitive detection of antigenic oligomeric carbohydrates in biological samples and is proposed as an alternative to quantitative enzyme-linked immunosorbent assay techniques. 相似文献
35.
Gijs M. ter Huurne Ghislaine Vantomme Bart W. L. van den Bersselaar Bala N. S. Thota Ilja K. Voets Anja R. A. Palmans E. W. Meijer 《Journal of polymer science. Part A, Polymer chemistry》2019,57(3):411-421
The supramolecular folding of amphiphilic heterograft copolymers equipped with dendritic pendants is investigated using a combination of proton nuclear magnetic resonance (1H NMR) spectroscopy, small‐angle X‐ray scattering, and circular dichroism spectroscopy. Hereto, the linear poly(ethylene glycol) pendants normally used to convey water compatibility are partially substituted with branched analogues. For one set of copolymers, second‐generation polyglycerol dendrons are directly attached to the polymer backbone, while for the other a hydrophilic linker is placed in between. The results show that the branching of the hydrophilic pendants affects the local structure of the folded copolymer but does not influence the overall conformation and single‐chain character of the folded copolymers in solution. All copolymers fold into 4–5 nm single‐chain polymeric nanoparticles with a very compact spherical morphology, independent of the dendritic content of the copolymer. Intriguingly, the incorporation of the dendritic pendants affects the formation of a structured interior even at low incorporation ratios. © 2018 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2019 , 57, 411–421 相似文献
36.
Simone Steig Frauke Cornelius Andreas Heise Rutger J. I. Knoop Gijs J. M. Habraken Cor E. Koning Henning Menzel 《Macromolecular Symposia》2007,248(1):199-206
A double-headed initiator was synthesized yielding two functional groups for the initiation of the nickel mediated ring-opening polymerization of γ-benzyl-L -glutamate-N-carboxyanhydride and controlled radical polymerization of vinyl monomers via ATRP or NMP. Well-defined block copolymers combining polypeptides and synthetic polymers were obtained. 相似文献
37.
A two-step sulfurization for efficient solution-phase synthesis of phosphorothioate oligonucleotides
Amar B.T. Ghisaidoobe Howard I. Duynstee Herman S. Overkleeft Gijs A. van der Marel 《Tetrahedron letters》2008,49(19):3129-3132
A solution-phase approach for the synthesis of phosphorothioate oligonucleotides that circumvents the use of chromatographic purifications of protected phosphorothioate intermediates was developed. Implementation of a two-step sulfurization protocol in the phosphoramidite method allows efficient isolation of the intermediate phosphorothioates by extractions. The viability of this approach is demonstrated by the synthesis of a hexameric phosphorothioate oligonucleotide fragment. 相似文献
38.
39.
Leeuwenburgh MA Geurink PP Klein T Kauffman HF van der Marel GA Bischoff R Overkleeft HS 《Organic letters》2006,8(8):1705-1708
[reaction: see text] A novel solid-phase synthesis strategy toward succinylhydroxamate peptides, using an appropriately protected hydroxamate building block, is described. Rapid and efficient access is gained to amine-functionalized peptides, which can be decorated with, for instance, a fluorescent label. In addition, we demonstrate an on-resin synthesis of a biotinylated photoactivatable hydroxamate peptide, which can be used as an activity-based probe for matrix metalloproteinases and ADAMs. 相似文献
40.
Rombouts Y Elass E Biot C Maes E Coddeville B Burguière A Tokarski C Buisine E Trivelli X Kremer L Guérardel Y 《Journal of the American Chemical Society》2010,132(45):16073-16084
Although lipo-oligosaccharides (LOSs) are recognized as major parietal components in many mycobacterial species, their involvement in the host-pathogen interactions have been scarcely documented. In particular, the biological implications arising from the high degree of structural species-specificity of these glycolipids remain largely unknown. Growing recognition of the Mycobacterium marinum-Danio rerio as a specific host-pathogen model devoted to the study of the physiopathology of mycobacterial infections prompted us to elucidate the structure-to-function relationships of the elusive end-product, LOS-IV, of the LOS biosynthetic pathway in M. marinum. Combination of physicochemical and molecular modeling methods established that LOS-IV resulted from the differential transfer on the caryophyllose-containing LOS-III of a family of very unusual N-acylated monosaccharides, naturally present as different diastereoisomers. In agreement with the partial loss of pathogenecity previously reported in a LOS-IV-deficient M. marinum mutant, we demonstrated that this terminal monosaccharide conferred to LOS-IV important biological functions, including macrophage activating properties. 相似文献