排序方式: 共有157条查询结果,搜索用时 187 毫秒
91.
Flores G Herraiz M Blanch GP Ruiz del Castillo ML 《Journal of chromatographic science》2007,45(1):33-37
The viability of using polydimethylsiloxane (PDMS) as a retaining material inside a programmed temperature vaporizer injector for the introduction of large-volume samples in gas chromatography is assessed. To that end, materials made up of Volaspher A-2 and coated with different percentages of PDMS (5%, 15%, and 50%) are considered. In addition, adsorbent (Tenax TA) and absorbent (PDMS) materials are comparatively studied in terms of their retention capacity. A relative standard deviation lower than 5.0% is obtained from the injection of PDMS, whereas values up to 49% are provided by Tenax TA. Significantly higher amounts of different volatile compounds are retained by PDMS in comparison with Tenax TA. In conclusion, the use of PDMS as a packing material seems to be viable for large-volume sampling and particularly recommendable for minor compounds occurring in complex matrices. 相似文献
92.
Hydroxyethylcellulose (HEC) and hydroxypropylcellulose (HPC) were studied by means of size exclusion chromatography with dual detection, i.e. employing simultaneously a refractive index (concentration sensitive) and a multiangle light scattering (molecular weight sensitive) detectors. The eluent was water and water solutions containing different concentrations of ionic salts. Molecular weight distributions and averages, coefficients of the scaling law of molecular dimensions and unperturbed dimensions were thus obtained from a single polydisperse sample of each polymer. Measurements were performed at 25 degrees C and the anomalous chromatographic behaviour, due to a combination of ion and size exclusion mechanisms, found when using pure water as eluent is transformed into a size exclusion mechanism by the addition of ionic salts. However, the two polymers behave on a different way in presence of salts. Thus, HEC, which is of low degree of substitution (DS), is close to theta conditions in the aqueous salt solutions (i.e. the q exponent of the scaling law has a value close to 0.5), whereas in the case of HPC the addition of salt improves the quality of the solvent up to a value of q around 0.6. Unperturbed dimensions are also calculated for both celluloses. 相似文献
93.
Dynamic binding capacity (DBC) measurements of cation-exchange resins were performed with two human monoclonal antibodies. DBC showed a pH dependent maximum, which was shifted to lower pH values with increasing buffer concentrations and increasing salting-out effect of the buffer anion according to the Hofmeister series. As this downshift correlates well with zeta potential values, a measurement of the latter allows the determination of the pH value for maximum DBC under a given set of conditions. Thus, the use of zeta potential values can accelerate the purification process development and helps to understand the protein adsorption mechanism. 相似文献
94.
95.
Gema Lizbeth Ramírez-Salinas Marlet Martínez-Archundia Jos Correa-Basurto Jazmín García-Machorro 《Molecules (Basel, Switzerland)》2020,25(23)
The worldwide health emergency of the SARS-CoV-2 pandemic and the absence of a specific treatment for this new coronavirus have led to the use of computational strategies (drug repositioning) to search for treatments. The aim of this work is to identify FDA (Food and Drug Administration)-approved drugs with the potential for binding to the spike structural glycoprotein at the hinge site, receptor binding motif (RBM), and fusion peptide (FP) using molecular docking simulations. Drugs that bind to amino acids are crucial for conformational changes, receptor recognition, and fusion of the viral membrane with the cell membrane. The results revealed some drugs that bind to hinge site amino acids (varenicline, or steroids such as betamethasone while other drugs bind to crucial amino acids in the RBM (naldemedine, atovaquone, cefotetan) or FP (azilsartan, maraviroc, and difluprednate); saquinavir binds both the RBM and the FP. Therefore, these drugs could inhibit spike glycoprotein and prevent viral entry as possible anti-COVID-19 drugs. Several drugs are in clinical studies; by focusing on other pharmacological agents (candesartan, atovaquone, losartan, maviroc and ritonavir) in this work we propose an additional target: the spike glycoprotein. These results can impact the proposed use of treatments that inhibit the first steps of the virus replication cycle. 相似文献
96.
Dr. Tania Neva Ana I. Carbajo-Gordillo Dr. Juan M. Benito Hugo Lana Dr. Gema Marcelo Prof. Carmen Ortiz Mellet Prof. Conchita Tros de Ilarduya Prof. Francisco Mendicuti Prof. José M. García Fernández 《Chemistry (Weinheim an der Bergstrasse, Germany)》2020,26(66):15259-15269
Original molecular vectors that ensure broad flexibility to tune the shape and surface properties of plasmid DNA (pDNA) condensates are reported herein. The prototypic design involves a cyclodextrin (CD) platform bearing a polycationic cluster at the primary face and a doubly linked aromatic module bridging two consecutive monosaccharide units at the secondary face that behaves as a topology-encoding element. Subtle differences at the molecular level then translate into disparate morphologies at the nanoscale, including rods, worms, toroids, globules, ellipsoids, and spheroids. In vitro evaluation of the transfection capabilities revealed marked selectivity differences as a function of nanocomplex morphology. Remarkably high transfection efficiencies were associated with ellipsoidal or spherical shapes with a lamellar internal arrangement of pDNA chains and CD bilayers. Computational studies support that the stability of such supramolecular edifices is directly related to the tendency of the molecular vector to form noncovalent dimers upon DNA templating. Because the stability of the dimers depends on the protonation state of the polycationic clusters, the coaggregates display pH responsiveness, which facilitates endosomal escape and timely DNA release, a key step in successful transfection. The results provide a versatile strategy for the construction of fully synthetic and perfectly monodisperse nonviral gene delivery systems uniquely suited for optimization schemes. 相似文献
97.
98.
99.
Beatriz Ballesteros Dr. Gema de la Torre Dr. Axel Shearer Anita Hausmann M. Ángeles Herranz Dr. Dirk M. Guldi Prof. Tomás Torres Prof. 《Chemistry (Weinheim an der Bergstrasse, Germany)》2010,16(1):114-125
A novel series of double‐decker lanthanide(III) bis(phthalocyaninato)–C60 dyads [LnIII(Pc)(Pc′)]–C60 (M=Sm, Eu, Lu; Pc=phthalocyanine) ( 1 a – c ) have been synthesized from unsymmetrically functionalized heteroleptic sandwich complexes [LnIII(Pc)(Pc′)] (Ln=Sm, Eu, Lu) 3 a – c and fulleropyrrolidine carboxylic acid 2 . The sandwich complexes 3 a – c were obtained by means of a stepwise procedure from unsymmetrically substituted free‐base phthalocyanine 5 , which was first transformed into the monophthalocyaninato intermediate [LnIII(acac)(Pc)] and further reacted with 1,2‐dicyanobenzene in the presence of 1,8‐diazabicyclo[5.4.0]undec‐7‐ene (DBU). 1H NMR spectra of the bis(phthalocyaninato) complexes 3 a – c and dyads 1 a – c were obtained by adding hydrazine hydrate to solutions of the complexes in [D7]DMF, a treatment that converts the free radical double‐deckers into the protonated species, that is, [LnIII(Pc)(Pc′)H] and [LnIII(Pc)(Pc′)H]–C60. The electronic absorption spectra of 3 a – c and 1 a – c in THF exhibit typical transitions of free‐radical sandwich complexes. In the case of dyads 1 a – c , the spectra display the absorption bands of both constituents, but no evidence of ground‐state interactions could be appreciated. When the UV/Vis spectra of 3 a – c and 1 a – c were recorded in DMF, typical features of the reduced forms were observed. Cyclic voltammetry studies for 3 a – c and 1 a – c were performed in THF. The electrochemical behavior of dyads 1 a – c is almost the exact sum of the behavior of the components, namely the double‐decker [LnIII(Pc)(Pc′)] and the C60 fullerene, thus confirming the lack of ground‐state interactions between the electroactive units. Photophysical studies on dyads 1 a – c indicate that only after irradiation at 387 nm, which excites both C60 and [LnIII(Pc)(Pc′)] components, a photoinduced electron transfer from the [LnIII(Pc)(Pc′)] to C60 occurs. 相似文献
100.
Gagliardo M Rodríguez G Dam HH Lutz M Spek AL Havenith RW Coppo P De Cola L Hartl F van Klink GP van Koten G 《Inorganic chemistry》2006,45(5):2143-2155
A palladium-catalyzed Stille coupling reaction was employed as a versatile method for the synthesis of a novel terpyridine-pincer (3, TPBr) bridging ligand, 4'-{4-BrC6H2(CH2NMe2)2-3,5}-2,2':6',2' '-terpyridine. Mononuclear species [PdX(TP)] (X = Br, Cl), [Ru(TPBr)(tpy)](PF6)2, and [Ru(TPBr)2](PF6)2, synthesized by selective metalation of the NCNBr-pincer moiety or complexation of the terpyridine of the bifunctional ligand TPBr, were used as building blocks for the preparation of heterodi- and trimetallic complexes [Ru(TPPdCl)(tpy)](PF6)2 (7) and [Ru(TPPdCl)2](PF6)2 (8). The molecular structures in the solid state of [PdBr(TP)] (4a) and [Ru(TPBr)2](PF6)2 (6) have been determined by single-crystal X-ray analysis. Electrochemical behavior and photophysical properties of the mono- and heterometallic complexes are described. All the above di- and trimetallic Ru complexes exhibit absorption bands attributable to (1)MLCT (Ru --> tpy) transitions. For the heteroleptic complexes, the transitions involving the unsubstituted tpy ligand are at a lower energy than the tpy moiety of the TPBr ligand. The absorption bands observed in the electronic spectra for TPBr and [PdCl(TP)] have been assigned with the aid of TD-DFT calculations. All complexes display weak emission both at room temperature and in a butyronitrile glass at 77 K. The considerable red shift of the emission maxima relative to the signal of the reference compound [Ru(tpy)2]2+ indicates stabilization of the luminescent 3MLCT state. For the mono- and heterometallic complexes, electrochemical and spectroscopic studies (electronic absorption and emission spectra and luminescence lifetimes recorded at room temperature and 77 K in nitrile solvents), together with the information gained from IR spectroelectrochemical studies of the dimetallic complex [Ru(TPPdSCN)(tpy)](PF6)2, are indicative of charge redistribution through the bridging ligand TPBr. The results are in line with a weak coupling between the {Ru(tpy)2} chromophoric unit and the (non)metalated NCN-pincer moiety. 相似文献