排序方式: 共有56条查询结果,搜索用时 15 毫秒
21.
Valeriya M. Trusova Todor Deligeorgiev Galyna Gorbenko 《Journal of fluorescence》2017,27(4):1359-1363
The present study was undertaken to design the novel liposomal drug formulation containing doxorubicin and europium coordination complexes. It was shown that co-encapsulation of the drugs facilitates the partitioning and permeation of lanthanides into the lipid bilayer. The obtained results suggest that new drug platform may have potential application in the design of novel antitumor agents. 相似文献
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Wendell Costa Bila Reysla Maria da Silveira Mariano Valmin Ramos Silva Maria Emília Soares Martins dos Santos Joel Alves Lamounier Eduardo Ferriolli 《Isotopes in environmental and health studies》2017,53(4):327-343
The main aim goal of this review was to gather information about recent publications related to deuterium oxide (D2O), and its use as a scientific tool related to human health. Searches were made in electronic databases Pubmed, Scielo, Lilacs, Medline and Cochrane. Moreover, the following patent databases were consulted: EPO (Espacenet patent search), USPTO (United States Patent and Trademark Office) and Google Patents, which cover researches worldwide related to innovations using D2O. 相似文献
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Olga K. Kutsenko Valeriya M. Trusova Galyna P. Gorbenko Anna S. Lipovaya Ekaterina I. Slobozhanina Lyudmila M. Lukyanenko Todor Deligeorgiev Aleksey Vasilev 《Journal of fluorescence》2013,23(6):1229-1237
The last decade has seen unprecedented upsurge of interest in the structural and toxic properties of particular type of protein aggregates, amyloid fibrils, associated with a number of pathological states. In the present study fluorescence spectroscopy technique has been employed to gain further insight into the membrane-related mechanisms of amyloid toxicity. To this end, erythrocyte model system composed of liposomes and hemoglobin was subjected to the action of oligomeric and fibrillar lysozyme. Acrylamide quenching of lysozyme fluorescence showed that solvent accessibility of Trp62 and Trp108 increases upon the protein fibrillization. Resonance energy transfer measurements suggested the possibility of direct complexation between hemoglobin and aggregated lysozyme. Using the novel squaraine dye SQ-1 it was demonstrated that aggregated lysozyme is capable of inhibiting lipid peroxidation processes. Fluorescent probes pyrene, Prodan and diphenylhexatriene were employed to characterize the membrane-modifying properties of hemoglobin and lysozyme. Both oligomeric and fibrillar forms of lysozyme were found to exert condensing influence on lipid bilayer structure, with the membrane effects of fibrils being less amenable to modulation by hemoglobin. 相似文献
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We report here a simple and efficient preparation of 1H-azepin-5(2H)-ones and their unexpectedly facile isomerization to 1H-azepin-5(4H)-ones under mildly basic reaction conditions. 相似文献
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Ioffe VM Gorbenko GP Tatarets AL Patsenker LD Terpechnig EA 《Journal of fluorescence》2006,16(4):547-554
The applicability of newly synthesized squarylium dye Sq to probing the changes in physical characteristics of lipid bilayer on the formation of protein-lipid complexes has been evaluated. Lipid vesicles composed of zwitterionic phospholipid phosphatidylcholine (PC) and its mixtures with positively charged detergent cetyltrimethylammonium bromide (CTAB), anionic phospholipid cardiolipin (CL), and cholesterol (Chol) were employed as lipid component of model membrane systems while protein constituent was represented by lysozyme (Lz). Fluorescence intensity of Sq was found to decrease on Lz association with lipid bilayer. This effect was observed in all kinds of model systems suggesting that Sq is sensitive to modification of lipid bilayer physical properties on hydrophobic protein-lipid interactions. It was found that Sq spectral response to variations in Chol content depends on relative contributions of electrostatic and hydrophobic components of Lz-membrane binding. 相似文献
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Ioffe VM Gorbenko GP Kinnunen PK Tatarets AL Kolosova OS Patsenker LD Terpetschnig EA 《Journal of fluorescence》2007,17(1):65-72
The applicability of the two newly commercial available squaraine labels Square-670-NHS and Seta-635-NHS to exploring protein-lipid
interactions has been evaluated. The labels were conjugated to lysozyme (Lz) (squaraine-lysozyme conjugates below referred
to as Square-670-Lz and Seta-635-Lz), a structurally well-characterized small globular protein displaying the ability to interact
both, electrostatically and hydrophobically with lipids. The lipid component of the model systems was represented by lipid
vesicles composed of zwitterionic lipids egg yolk phosphatidylcholine (PC) and 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (SOPC), and their mixtures with anionic lipids either beef heart cardiolipin (CL) or 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG), respectively. Fluorescence intensity of Square-670-Lz was found to decrease upon association
with lipid bilayer, while the fluorescence intensity of Seta-635-Lz displayed more complex behavior depending on lipid-to-protein
molar ratio. Covalent coupling of squaraine labels to lysozyme exerts different influence on the properties of dye-protein
conjugate. It was suggested that Square-670-NHS covalent attachment to Lz molecule enhances protein propensity for self-association,
while squaraine label Seta-635-NHS is sensitive to different modes of lysozyme-lipid interactions—within the L:P range 6–11,
when hydrophobic protein-lipid interactions are predominant, an aggregation of membrane-bound protein molecules takes place,
thereby decreasing the fluorescence intensity of Seta-635-Lz. At higher L:P values (from 22 to 148) when electrostatic interactions
are enhanced fluorescence intensity of Seta-635-Lz increases with increasing lipid concentrations. 相似文献
29.
Galyna Volynets Hanna Vyshniakova Georgiana Nitulescu George Mihai Nitulescu Anca Ungurianu Denisa Margina Olena Moshynets Volodymyr Bdzhola Ihor Koleiev Olga Iungin Sergiy Tarnavskiy Sergiy Yarmoluk 《Molecules (Basel, Switzerland)》2021,26(23)
Staphylococcus aureus (S. aureus) is a causative agent of many hospital- and community-acquired infections with the tendency to develop resistance to all known antibiotics. Therefore, the development of novel antistaphylococcal agents is of urgent need. Sortase A is considered a promising molecular target for the development of antistaphylococcal agents. The main aim of this study was to identify novel sortase A inhibitors. In order to find novel antistaphylococcal agents, we performed phenotypic screening of a library containing 15512 compounds against S. aureus ATCC43300. The molecular docking of hits was performed using the DOCK program and 10 compounds were selected for in vitro enzymatic activity inhibition assay. Two inhibitors were identified, N,N-diethyl-N′-(5-nitro-2-(quinazolin-2-yl)phenyl)propane-1,3-diamine (1) and acridin-9-yl-(1H-benzoimidazol-5-yl)-amine (2), which decrease sortase A activity with IC50 values of 160.3 µM and 207.01 µM, respectively. It was found that compounds 1 and 2 possess antibacterial activity toward 29 tested multidrug resistant S. aureus strains with MIC values ranging from 78.12 to 312.5 mg/L. These compounds can be used for further structural optimization and biological research. 相似文献
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Valeriya M. Trusova Galyna P. Gorbenko Todor Deligeorgiev Nikolai Gadjev Aleksey Vasilev 《Journal of fluorescence》2009,19(6):1017-1023
A novel squaraine probe SQ-1 has been found to be appropriate for monitoring the peroxidation processes in membrane systems.
Formation of free radicals was triggered by methemoglobin (metHb) or cytochrome c (cyt c) binding to the model lipid membranes composed of zwitterionic lipid phosphatidylcholine (PC) and anionic lipid cardiolipin
(CL). Protein association with the lipid vesicles was followed by drastic quenching of SQ-1 fluorescence. The observed spectral
changes were suppressed in the presence of free radical scavengers, butylated hydroxytoluene (BHT) and thiourea (TM) suggesting
that SQ-1 decolorization can be attributed to its reactions with lipid radicals. 相似文献