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排序方式: 共有187条查询结果,搜索用时 375 毫秒
11.
Muddaser Shah Waheed Murad Najeeb Ur Rehman Sobia Ahsan Halim Manzoor Ahmed Hazir Rehman Muhammed Zahoor Sidra Mubin Ajmal Khan Mohamed A. Nassan Gaber El-Saber Batiha Ahmed Al-Harrasi 《Molecules (Basel, Switzerland)》2021,26(12)
In the current study, in vitro antimicrobial and antioxidant activities and in vivo anti-inflammatory and analgesic activities of Scutellaria edelbergii Rech. f. (crude extract and subfractions, i.e., n-hexane, ethyl acetate (EtOAc), chloroform, n-butanol (n-BuOH) and aqueous) were explored. Initially, extraction and fractionation of the selected medicinal plant were carried out, followed by phytochemical qualitative tests, which were mostly positive for all the extracts. EtOAc fraction possessed a significant amount of phenolic (79.2 ± 0.30 mg GAE/g) and flavonoid (84.0 ± 0.39 mg QE/g) content. The EtOAc fraction of S. edelbergii exhibited appreciable antibacterial activity against Gram-negative (Escherichia coli and Klebsiella pneumoniae) strains and significant zones of inhibition were observed against Gram-positive bacterial strains (Bacillus subtilis and Staphylococcus aureus). However, it was found inactive against Candida Albicans and Fusarium oxysporum fungal strains. The chloroform fraction was the most effective with an IC50 value of 172 and 74 µg/mL against DPPH (1,1-Diphenyl-2-picryl-hydrazyl) and ABTS assays, in comparison with standard ascorbic acid 59 and 63 µg/mL, respectively. Moreover, the EtOAc fraction displayed significant in vivo anti-inflammatory activity (54%) using carrageenan-induced assay and significant (55%) in vivo analgesic activity using acetic acid-induced writing assay. In addition, nine known compounds, ursolic acid (UA), ovaul (OV), oleanolic acid (OA), β-sitosterol (BS), micromeric acid (MA), taraxasterol acetate (TA), 5,3′,4′-trihydroxy-7-methoxy flavone (FL-1), 5,7,4′-trihydroxy-6,3′-dimiethoxyflavone (FL-2) and 7-methoxy catechin (FL-3), were isolated from methanolic extract of S. edelbergii. These constituents have never been obtained from this source. The structures of all the isolated constituents were elucidated by spectroscopic means. In conclusion, the EtOAc fraction and all other fractions of S. edelbergii, in general, displayed a significant role as antibacterial, free radical scavenger, anti-inflammatory and analgesic agents which may be due to the presence of these constituents and other flavonoids. 相似文献
12.
JPC – Journal of Planar Chromatography – Modern TLC - A mobile phase system comprising of ethyl acetate and propionic acid in 1:1 (v/v) ratio was identified as the most suitable green... 相似文献
13.
Salwinder Singh Dhaliwal Vivek Sharma Arvind Kumar Shukla Vibha Verma Manmeet Kaur Yashbir Singh Shivay Shahida Nisar Ahmed Gaber Marian Brestic Viliam Barek Milan Skalicky Peter Ondrisik Akbar Hossain 《Molecules (Basel, Switzerland)》2022,27(4)
Globally, many developing countries are facing silent epidemics of nutritional deficiencies in human beings and animals. The lack of diversity in diet, i.e., cereal-based crops deficient in mineral nutrients is an additional threat to nutritional quality. The present review accounts for the significance of biofortification as a process to enhance the productivity of crops and also an agricultural solution to address the issues of nutritional security. In this endeavor, different innovative and specific biofortification approaches have been discussed for nutrient enrichment of field crops including cereals, pulses, oilseeds and fodder crops. The agronomic approach increases the micronutrient density in crops with soil and foliar application of fertilizers including amendments. The biofortification through conventional breeding approach includes the selection of efficient genotypes, practicing crossing of plants with desirable nutritional traits without sacrificing agricultural and economic productivity. However, the transgenic/biotechnological approach involves the synthesis of transgenes for micronutrient re-translocation between tissues to enhance their bioavailability. Soil microorganisms enhance nutrient content in the rhizosphere through diverse mechanisms such as synthesis, mobilization, transformations and siderophore production which accumulate more minerals in plants. Different sources of micronutrients viz. mineral solutions, chelates and nanoparticles play a pivotal role in the process of biofortification as it regulates the absorption rates and mechanisms in plants. Apart from the quality parameters, biofortification also improved the crop yield to alleviate hidden hunger thus proving to be a sustainable and cost-effective approach. Thus, this review article conveys a message for researchers about the adequate potential of biofortification to increase crop productivity and nourish the crop with additional nutrient content to provide food security and nutritional quality to humans and livestock. 相似文献
14.
Gorazd Hribar Andrej Žnidaršič Marjan Bele Uroš Maver Simon Caserman Miran Gaberšček Vladka Gaberc-Porekar 《Journal of nanoparticle research》2011,13(7):3019-3032
The authors’ intention was to prepare nanometer-sized zinc-phosphate nanoparticles that would be capable of binding histidine-rich
TNF-α analogs onto their surface via a coordinative bond. Zinc-phosphate nanoparticles with a size of around 60 nm were prepared
by a wet precipitation method and characterized using SEM, EDX, XRD, and DLS. First, BSA was bound as a testing protein, afterward
two TNF-α analogs with decreased activity were bound to the described nanoparticles. The efficiency of binding and the existence
of coordinative bond were confirmed with SDS-PAGE analysis. During binding, particle storage, and release experiments, the
prepared TNF-α analogs retained their biological activity—hence the epitopes necessary for formation of antibodies stayed
intact. The particle size did not change within a period of 2 weeks. No significant agglomeration was observed, the particles
could be quickly dispersed in ultrasound. The present nanoparticles and the general approach of coordinative binding are widely
applicable for natural and engineered histidine-rich proteins. The nanoparticles bearing appropriate TNF-α analogs could also
be potentially used for active immunotherapy to tackle the chronic inflammatory diseases associated with pathogenically elevated
levels of TNF-α. 相似文献
15.
In need for nonperturbing but spectroscopically unique environments for studies of lipid/lipid and lipid/protein interactions, we have investigated the thermodynamic properties of deuterated and undeuterated dipalmitoyl phosphatidylcholines as well as the mixtures of both. The data have been collected using the highly sensitive differential scanning calorimetry. From the results of these measurements we conclude that mixtures of deuterated and undeuterated lipids behave ideally. The transition enthalpies, transition entropies and the cooperative unit are given as a function of the input ratio of the components and the phase diagram is constructed, showing that the deuterated lipids are suitable as nonperturbing probes. 相似文献
16.
A novel series of pyrazolo[1,5-a]pyrimidines 14a–j and pyrazolo[1,5-a]quinazolines 18a, b were synthesized via condensation of 5-amino-1H-pyrazoles 10a, b with 3-(dimethylamino)-1-aryl-prop-2-en-1-ones 11a–e and 2-((dimethylamino)methylene)-5,5-dimethylcyclohexane-1,3-dione (15), respectively, in glacial acetic acid. Finally, treatment of 10a, b with sodium nitrite (NaNO2) afforded pyrazolo[3,4-d]triazines 20a, b. Structures of compounds were confirmed by their spectral data. These compounds were screened for their in vitro cytotoxic activities against human cancer cell lines (HepG-2 and MCF-7) using 3-[4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The results reveal that, the compounds 14b and 14h were the most potent in comparison with doxorubicin. The structure–activity relationship was discussed. 相似文献
17.
Janko Drnovšek Igor Pušnik Jovan Bojkovski Gaber Begeš 《Accreditation and quality assurance》2010,15(2):125-131
The article describes the project of regional cooperation in proficiency testing within CARDS 2006 (assistance programme to
the Western Balkans), which was coordinated by European Committee for Standardization, European Commission and European Free
Trade Association and conducted by the University of Ljubljana, Faculty of Electrical Engineering, Laboratory of Metrology
and Quality. The later is the holder of the national standard for thermodynamic temperature as well as an accredited proficiency
testing provider and also accredited as a calibration, testing and inspection laboratory. It is also responsible for graduate
study programme Quality Engineering. Proficiency testing was considered as a core activity of the entire project, but additionally
as a very appropriate platform for knowledge transfer of EU technical legislation in selected sectors, metrology, testing
and quality assurance in general. The project had a high added value for participating laboratories in countries of Western
Balkans, because it required an active role of all participating laboratories, assured participation of staff from national
metrology institutions, all national accreditation bodies, and was supported by national quality infrastructures, such as
standardization, governmental institutions etc. in the entire Western Balkans region. The activities stimulated progress in
the area of conformity assessment, added knowledge to the implementation of technical directives and development of the technical
part of national legislation and therefore facilitated the process toward the accession to the European Union. The main objective
was therefore to organize proficiency testing in those sectors (construction, consumer protection and environment) that were
vital in the pre-accession process of countries seeking full implementation of EU technical legislation. 相似文献
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