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排序方式: 共有188条查询结果,搜索用时 15 毫秒
101.
We evaluate the prospects for finding evidence of dark matter production at the CERN Large Hadron Collider. We consider weakly interacting massive particles (WIMPs) and superWIMPs and characterize their properties through model-independent parametrizations. The observed relic density then implies lower bounds on dark matter production rates as functions of a few parameters. For WIMPs, the resulting signal is indistinguishable from background. For superWIMPs, however, this analysis implies significant production of metastable charged particles. For natural parameters, these rates may far exceed Drell-Yan cross sections and yield spectacular signals. 相似文献
102.
In a previous report (J. Fluoresc. 16, 153, 2006) we studied the chaotropiclly induced denaturation of Bovine Serum Albumin (BSA) using the fluorescence decay
kinetics at different stages in the denaturation of BSA by guanidinium hydrochloride (GuHCl). In this work, we gain a more
detailed insight into the BSA denaturation process by investigating the thermodynamics of the process. Structural changes
were monitored spectrophotometrically via the intrinsic protein fluorescence from tryptophan residues, and the extrinsic fluorescence
from 1,8-anilinonaphthalene sulphonate (ANS). ANS tends to locate in a variety of binding sites in BSA which are located in
different domains, and these can be selectively populated using different, 1:1 and 1:10 molar ratios of BSA to ANS. The data
from steady-state and time-resolved fluorescence spectroscopy were analyzed using thermodynamic two-state and three-state
models and the lifetime data clearly indicated the presence of an intermediate state during denaturation. A global analysis
using non-linear regression gave a
DGH2O,D0 = 6.7 \textkcal.\textmo\textl - 1 \Delta G_{{H_2}O,D}^0 = 6.7\;{\text{kcal}}.{\text{mo}}{{\text{l}}^{ - 1}} for the complete unfolding of the BSA-ANS complexes, and a
DGH2O,I0 = 0.9 \textkcal.\textmo\textl - 1 \Delta G_{{H_2}O,I}^0 = 0.9\;{\text{kcal}}.{\text{mo}}{{\text{l}}^{ - 1}} for the first step to the intermediate. Therefore, the unfolding energy of the intermediate, which appears mostly at intermediate
GuHCl concentrations (1.0 to 1.5 M), to the denatured state, is 5.8 kcal.mol−1. The lifetime analysis of the BSA-ANS complexes also shows clearly that there are differences in stability of the BSA domains,
with domain III unfolding first at low GuHCl concentrations (<1.5 M). 相似文献
103.
Denisio M. Togashi Alan G. Ryder Gregor Heiss 《Colloids and surfaces. B, Biointerfaces》2009,72(2):219-229
The quantification and analysis of protein adsorption on solid surfaces are of significant importance in many areas of biosensors, biomaterials, and biomedical devices research. The accurate, in situ, measurement of multiple physicochemical properties from the thin protein layers which adsorb on surfaces is critical to understanding biocompatibility, surface chemistry factors, and the performance of implanted medical devices. To implement such studies, new tools and simple protocols based on instrumentation available in typical bioscience laboratories are desirable. In this work, we have developed an approach using confocal fluorescence microscopy to quantify the amount of bovine serum albumin (BSA) adsorbed onto a flat hydrophilic glass surface, under different pH conditions. This approach which can be implemented using most confocal fluorescence microscopes is described in detail and its limitations are discussed. This quantitative method coupled with the Langmuir model allowed for the determination of adsorption parameters at pH 2.0, 4.0, 7.4, and 9.2. The adsorption parameters were validated by comparison with literature values obtained from different techniques for a similar protein–surface system. The Derjaguin–Landau–Verwey–Overbeek (DLVO) theory was then used for a detailed analysis of these parameters, to understand in general terms how pH affected the surface adsorption interactions. 相似文献
104.
Fumihiro Toyokawa Ken‐Ichi Fukukawa Yuji Shibasaki Shinji Ando Mitsuru Ueda 《Journal of polymer science. Part A, Polymer chemistry》2005,43(12):2527-2535
A novel poly(σ‐hydroxyamide) (PHA) based photosensitive polymer that exhibits high transparency at 365 nm wavelength (i‐line) has been developed. Time‐dependent density functional theory (TD‐DFT) calculations using the B3LYP hybrid functional were performed to predict the transparencies of various hydroxyamides in the i‐line region. Based on the calculations, 4,4′‐sulfonylbis(σ‐aminophenol) (SAP) was prepared and polymerized with 4,4′‐oxybis(benzoyl chloride) (OBBC), and the resulting PHA, which is abbreviated as PHA‐S, showed a high transparency comparable to that of PHA derived from 4,4′‐(hexafluoroisopropylidene)bis(σ‐aminophenol). Positive‐type photosensitive PHA was then formulated based on PHA‐S with a crosslinker 1,3,5‐tris[(2‐vinyloxy)ethoxy]benzene (TVEB) and a photoacid generator (5‐propylsulfonyloxyimino‐5H‐thiophen‐2‐ylidene)‐2‐(methylphenyl)acetonitrile (PTMA) (17:3:1 in weight ratio), and demonstrated photosensitivity and contrast of 14 mJ/cm2 and 2.7, respectively, when the resist film was prebaked at 120 °C for 5 min, irradiated by i‐line, post exposure baked at 120 °C for 5 min, developed with an 2.38 wt% TMAH solution for 5 s. A clear positive image featuring 10‐μm line‐and‐space was also printed in a film which was exposed to 50 mJ/cm2 of i‐line by contact‐printing. © 2005 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 43: 2527–2535, 2005 相似文献
105.
106.
Cytotoxic bibenzyls, and germacrane- and pinguisane-type sesquiterpenoids have been isolated from unidentified Indonesian and Tahitian Frullania sp. and Japanese Porella perrottetiana by using a combination of chromatographic methods. The structure activity relationship (SAR) study showed that the presence of a phthalide group in bibenzyls, an alpha-methylene-gamma-lactone in germacrane-type sesquiterpenoids, and beta-hydroxycarbonyl in pinguisane-type sesquiterpenoids play an important role in providing cytotoxic activity against both human promyelocytic leukemia (HL-60) and human pharyngeal squamous carcinoma (KB) cell lines. The structure of each isolated compound was elucidated by using spectroscopic methods and the cytotoxicity was determined by using the WST-8 colorimetric assay. 相似文献
107.
Fumitaka YoshimuraKazutaka Nakamura Fumihiro WakaiMasahiko Hara Mamoru YoshimotoOsamu Odawara Hiroyuki Wada 《Applied Surface Science》2011,257(6):2170-2175
We have successfully prepared a novel nanoparticle solution of Sr2MgSi2O7: Eu2+, Dy3+ with afterglow properties by means of laser ablation in liquid. This process also produced by-products of different kinds, depending on the liquid used. The amount of by-product and the size of the nanoparticles were controlled by the energy density of laser ablation. The amount of by-product was reduced by a decrease in the energy density, which also decreased the particle size of the nanoparticles. The PL spectrum of the nanoparticles was the same as that of the target materials used for laser ablation. The afterglow properties deteriorated with a decrease in particle size. We concluded that an increase in specific surface area caused by a decrease in particle size resulted in the decrease of luminescent intensity. 相似文献
108.
Teruo Ushijima Kyousuke Handa Homare Nishizaki Fumihiro Chiba 《Journal of Computational and Applied Mathematics》2009,232(1):17-45
This paper concerns a determination procedure for conformal mapping of a wing through a finite element computation of potential function associated with the flow of 2-dimensional perfect fluid around the given wing section. Through our numerical procedure a family of mappings is obtained in the forms of finite Laurent series for an initial wing section input. Each member of the family describes a wing section located in a neighboring domain of the input one. Some of them could be expected as modified versions of the original wing section input, although they could not recover completely it.Inputting the shape of wing section has ambiguity in practical cases of wing sections such as the NACA23012 wing section. We would like to postulate that our identification procedure should be employed in the determination process of numerical profiles of the wing section considered, since identified ones are significantly easier in numerical processing than the original input shape. 相似文献
109.
The objective of this research effort was to investigate the quasi-steady flow field produced by explosives in confined facilities.
In this effort we modeled tests in which a high explosive (HE) cylindrical charge was hung in the center of a room and detonated.
The HEs used for the tests were C-4 and AFX 757. While C-4 is just slightly under-oxidized and is typically modeled as an
ideal explosive, AFX 757 includes a significant percentage of aluminum particles, so long-time afterburning and energy release
must be considered. The Lawrence Livermore National Laboratory (LLNL)-produced thermo-chemical equilibrium algorithm, “Cheetah”,
was used to estimate the remaining burnable detonation products. From these remaining species, the afterburning energy was
computed and added to the flow field. Computations of the detonation and afterburn of two HEs in the confined multi-room facility
were performed. The results demonstrate excellent agreement with available experimental data in terms of blast wave time of
arrival, peak shock amplitude, reverberation, and total impulse (and hence, total energy release, via either the detonation
or afterburn processes. 相似文献
110.
Crude extracts of the Tahitian liverworts Mastigophora diclados and Frullania sp., the Indonesian Frullania sp., Dumortiera hirsuta and Marchantia sp., and the Japanese Porella perrottetiana were investigated chemically by using gas chromatography-mass spectrometry (GC-MS). All extracts contained various volatile sesqui- and diterpenoids and a few aromatic compounds. The Tahitian M. diclados and Frullania sp., and the Indonesian Frullania sp. exhibited cytotoxic activity against HL-60 and KB cell lines. The extracts of the Tahitian M. diclados and the Indonesian Marchantia sp. showed radical scavenging activity, whereas the crude extracts of the Tahitian M. diclados and Frullania sp., and the Indonesian Frullania and Marchantia sp. showed antimicrobial activity against Staphylococcus aureus and Bacillus subtilis. 相似文献