全文获取类型
收费全文 | 608篇 |
免费 | 13篇 |
国内免费 | 1篇 |
专业分类
化学 | 402篇 |
晶体学 | 5篇 |
力学 | 1篇 |
数学 | 32篇 |
物理学 | 182篇 |
出版年
2022年 | 3篇 |
2021年 | 5篇 |
2020年 | 5篇 |
2019年 | 10篇 |
2018年 | 4篇 |
2017年 | 6篇 |
2016年 | 16篇 |
2015年 | 11篇 |
2014年 | 25篇 |
2013年 | 28篇 |
2012年 | 29篇 |
2011年 | 36篇 |
2010年 | 21篇 |
2009年 | 35篇 |
2008年 | 36篇 |
2007年 | 25篇 |
2006年 | 33篇 |
2005年 | 37篇 |
2004年 | 27篇 |
2003年 | 27篇 |
2002年 | 23篇 |
2001年 | 10篇 |
2000年 | 17篇 |
1999年 | 9篇 |
1998年 | 6篇 |
1997年 | 4篇 |
1996年 | 8篇 |
1995年 | 6篇 |
1994年 | 4篇 |
1993年 | 8篇 |
1992年 | 9篇 |
1991年 | 8篇 |
1990年 | 5篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 8篇 |
1986年 | 5篇 |
1985年 | 3篇 |
1984年 | 9篇 |
1982年 | 4篇 |
1981年 | 3篇 |
1980年 | 9篇 |
1979年 | 3篇 |
1978年 | 5篇 |
1977年 | 5篇 |
1976年 | 3篇 |
1975年 | 7篇 |
1974年 | 5篇 |
1973年 | 5篇 |
1970年 | 2篇 |
排序方式: 共有622条查询结果,搜索用时 15 毫秒
101.
John?Mann Dezheng?Sun Quan?Ma Jen-Ru?Chen Edwin?Preciado Taisuke?Ohta Bogdan?Diaconescu Koichi?Yamaguchi Tai?Tran Michelle?Wurch KatieMarie?Magnone Tony F.?Heinz Gary L.?Kellogg Roland?Kawakami Ludwig?BartelsEmail author 《The European Physical Journal B - Condensed Matter and Complex Systems》2013,86(5):226
Areas of single-layer MoS2 film can be prepared in a tube furnace without the need for temperature control. The films were characterized by means of Raman spectroscopy, photoluminescence, low-energy electron diffraction and microscopy, and X-ray photoelectron spectroscopy and mapping. Transport measurements show n-doped material with a mobility of 0.26 cm2 V-1 s-1. 相似文献
102.
Zhaoma Shu Iku Tanaka Azumi Ota Daichi Fushihara Naoko Abe Saki Kawaguchi Kosuke Nakamoto Fumiaki Tomoike Seiichi Tada Yoshihiro Ito Yasuaki Kimura Hiroshi Abe 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(20):6683-6687
Development of intracellular delivery methods for antisense DNA and siRNA is important. Previously reported methods using liposomes or receptor‐ligands take several hours or more to deliver oligonucleotides to the cytoplasm due to their retention in endosomes. Oligonucleotides modified with low molecular weight disulfide units at a terminus reach the cytoplasm 10 minutes after administration to cultured cells. This rapid cytoplasmic internalization of disulfide‐modified oligonucleotides suggests the existence of an uptake pathway other than endocytosis. Mechanistic analysis revealed that the modified oligonucleotides are efficiently internalized into the cytoplasm through disulfide exchange reactions with the thiol groups on the cellular surface. This approach solves several critical problems with the currently available methods for enhancing cellular uptake of oligonucleotides and may be an effective approach in the medicinal application of antisense DNA and siRNA. 相似文献
103.
Y. Kawakami S. Iwai N. Yoneyama N. Kobayashi 《Journal of Physics and Chemistry of Solids》2008,69(12):3085-3088
Ultrafast dynamics of the photoinduced insulator (I) to metal (M) transition were investigated using femtosecond mid-infrared pump-probe spectroscopy in two-dimensional organic Mott insulators [bis (ethylenedithio)]-tetrathiafulvalene (BEDT-TTF) salts κ-(BEDT-TTF)2X (κ-(ET)2X, where X denotes anion). In κ-(d-ET)2Cu[N(CN)2]Br, a metallic state was photogenerated using a phonon-mediated mechanism: the effective bandwidth increases through the photoinduced molecular rearrangement. The mechanism differs fundamentally from the previously reported photoinduced filling control in one-dimensional Mott insulators. 相似文献
104.
Dr. Hugh Nakamura Manami Kawakami Dr. Chihiro Tsukano Prof. Dr. Yoshiji Takemoto 《Chemistry (Weinheim an der Bergstrasse, Germany)》2019,25(37):8701-8704
A concise route for construction of the ACDE ring skeleton in calyciphylline A type alkaloids was developed using an intramolecular [5+2] cycloaddition reaction of an oxidopyrylium species bearing a tetrasubstituted olefin. Key to the success of this reaction was the combination of acid and base, which accelerated the construction of this skeleton containing a spiro ring and vicinal quaternary carbon centers. The resultant tricyclic ADE ring compound was converted to an ACDE ring model through C−H oxidation and an aza-Wittig reaction. 相似文献
105.
Simultaneous determination of erlotinib and its isomeric major metabolites in human plasma using isocratic liquid chromatography–tandem mass spectrometry and its clinical application
下载免费PDF全文
![点击此处可从《Biomedical chromatography : BMC》网站下载免费的PDF全文](/ch/ext_images/free.gif)
This study developed a method for the simultaneous determination of erlotinib and its isomeric major metabolites, OSI‐413 and OSI‐420, in human plasma using an isocratic liquid chromatography–tandem mass spectrometry. Plasma specimens deproteinized with acetonitrile were separated using a 3‐µm particle size octadecylsilyl column. The m/z values of the precursor and product ions for the analytes were as follows: erlotinib, 394.2/278.2; and OSI‐413 and OSI‐420, 380.2/278.2. The total run time was 21 min and no peaks interfering with the analytes and internal standard (d6‐erlotinib) in human plasma were observed. The calibration curves of erlotinib, OSI‐413 and OSI‐420 were linear over the concentration ranges of 10–3000, 2–500 and 2–100 ng/mL, respectively. The pretreatment recovery ratios were >86.1%. The intra‐ and inter‐assay precisions and accuracies were <12.7 and 89.0–108.9% for all analytes. This validated method was applied to the determination of plasma samples in lung cancer patients receiving 150 mg of oral erlotinib. The plasma concentration ranges of erlotinib, OSI‐413 and OSI‐420 were 373–2354, 15.7–379 and 2.5–43.6 ng/mL, respectively. In conclusion, the present method can be helpful for evaluating the plasma exposures of erlotinib and its major isomeric metabolites in clinical settings. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
106.
Simple and validated UHPLC method coupled to UV detection for determination of daptomycin in human plasma and urine
下载免费PDF全文
![点击此处可从《Biomedical chromatography : BMC》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Daptomycin, a lipopeptide antibiotic with excellent activity against Gram‐positive bacteria, is excreted primarily by the kidneys. Development of effective chromatographic methodologies for the determination of daptomycin in human specimens is necessary for clinical use. This study developed a simple and validated ultra‐high‐performance liquid chromatography method coupled to ultraviolet detection for determination of daptomycin in human plasma and urine. After the pretreatments involving protein precipitation, the supernatants were separated using a 2.3 µm particle size octadecylsilyl column, and the run time was 1 min. The calibration curves were linear over the concentration ranges of 2–200 mg/L for plasma and 25–300 mg/L for urine. Intra‐ and inter‐assay precision and accuracy values of plasma were within 13.5 and 92–100% and within 10.7 and 100–107%, respectively. Those of urine were within 5.0 and 101–104% and within 3.7 and 100–101%, respectively. The validated method was applied to the determination of plasma and urine samples in patients receiving 4–6 mg/kg of intravenous daptomycin, resulting in sufficient sensitivity for evaluating the plasma exposure and urinary excretion. In conclusion, the present method with acceptable analytical performance can be helpful for evaluating the pharmacokinetic disposition of daptomycin in clinical settings. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
107.
Dr. Norifumi Kawakami Hiroki Kondo Yuki Matsuzawa Kaoru Hayasaka Erika Nasu Kenji Sasahara Dr. Ryoichi Arai Prof. Kenji Miyamoto 《Angewandte Chemie (International ed. in English)》2018,57(38):12400-12404
Protein‐based nanoparticles hold promise for a broad range of applications. Here, we report the production of a uniform anionic hollow protein nanoparticle, designated TIP60, which spontaneously assembles from a designed fusion protein subunit based on the geometric features of polyhedra. We show that TIP60 tolerates mutation and both its interior and exterior surfaces can be chemically modified. Moreover, TIP60 forms larger structures upon the addition of a cationic protein. Therefore, TIP60 can be used as a modifiable nano‐building block for further molecular assembly. 相似文献
108.
Chieko Kogawa Asuka Fujiwara Ryuta Sekiguchi Taku Shoji Jun Kawakami Masaaki Okazaki Shunji Ito 《Tetrahedron》2018,74(49):7018-7029
Azulene analog of tryptanthrin, azuleno[1′,2′:4,5]pyrrolo[2,1-b]quinazoline-6,14-dione, was successfully prepared by the condensation reaction of azuleno[2,1-b]pyrrole-2,3-dione with isatoic anhydride in the presence of sodium hydride or diisopropylethylamine (DIPEA). Its 2-halo derivatives were also obtained in high yields by the condensation reaction with 5-haloisatoic anhydrides in the presence of DIPEA. Reactivity toward electrophilic reagents was revealed by halogenation with N-bromosuccinimide (NBS) or N-iodosuccinimide (NIS) to afford 12-halo derivatives in high yields. Among the halo derivatives, 2-iodo and 12-iodo derivatives were reactive enough to afford phenylethynyl derivatives under Pd-catalyzed Sonogashira cross-coupling conditions. Within the phenylethynyl derivatives, only 12-phenylethynyl derivative was transformed into its 1,1,4,4-tetracyanobutadiene (TCBD) derivative by the reaction with TCNE. Amphoteric redox properties of the novel azulene analogs of tryptanthrin were characterized by spectroscopic and voltammetric analyses. 相似文献
109.
Six new triterpene glycosides stryphnosides A-F (1-6) were isolated from the pericarps of Stryphnodendron fissuratum (Leguminosae). The structures of 1-6 were determined on the basis of extensive spectroscopic analysis, including two-dimensional (2D) NMR data, and the results of hydrolytic cleavage. The sugar moieties of 3-6 are very unique in structure having not only novel sugar sequences but also the terminal α-l-arabinopyranosyl unit with a 1C4 conformation. Stryphnosides C (3)-F (6) are the first representative of naturally occurring glycosides with the 1C4 terminal α-l-arabinopyranosyl group. 相似文献
110.
Hiraka K Kanehisa M Tamai M Asayama S Nagaoka S Oyaizu K Yuasa M Kawakami H 《Colloids and surfaces. B, Biointerfaces》2008,67(1):54-58
We prepared an anticancer drug based on a pH-sensitive liposome retaining Fe-porphyrin as an SOD mimic. The liposomes contained cationic/anionic lipid combinations and were composed of Fe-porphyrin, 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine, dimethylditetradecylammonium bromide, sodium oleate, and Tween-80. The Fe-porphyrin was released from the liposome at low pH, and the cytotoxicity for cancer cells by the liposome depended on the acidic environments of the endosomes in the cells. Furthermore, although the liposome exhibited an excellent anticancer effect on a gastric cancer cell line, the SOD activity of Fe-porphyrin was shown to have a significant influence on the cytotoxicity toward cancer cells. These findings suggest that the pH-sensitive liposome retaining the Fe-porphyrin as an SOD mimic promises to be a novel anticancer drug for endosomal escape. 相似文献